Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 182 57.1 1926220 99.1% 1908884 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,455,581 Δ1 bp coding (29/999 nt) fbp ← fructose‑1,6‑bisphosphatase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,455,5780T.100.0% 236.9 / NA 56coding (32/999 nt)fbpfructose‑1,6‑bisphosphatase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (29/27);  total (29/27)

GTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCTTTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGA  >  NC_000913/4455442‑4455709
                                                                                                                                        |                                                                                                                                   
gTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  <  1:809077/140‑2 (MQ=255)
   ctcAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttcgac                                                                                                                               <  1:54321/140‑5 (MQ=255)
     cAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACaa                                                                                                                            <  2:363820/140‑1 (MQ=255)
             cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  <  1:332725/127‑2 (MQ=255)
             cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  >  2:332725/1‑126 (MQ=255)
             cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATaaa                                                                                                                        <  1:514885/136‑1 (MQ=255)
             cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATaaa                                                                                                                        >  2:514885/1‑136 (MQ=255)
                     cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCt                                                                                                                 <  1:860293/135‑1 (MQ=255)
                     cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCt                                                                                                                 >  2:860293/1‑135 (MQ=255)
                     cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGt                                                                                                            <  2:807318/140‑1 (MQ=255)
                                  aGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACtt                                                                                               >  1:751/1‑140 (MQ=255)
                                  aGTCCTGCTTTGTTGATATCGAGATGGATAATCTTGACGCCAAGTTTTATTGCCGACAGCAAAGGAGTGAGCTCACCGGAAACAACAAAAACCTCGCGATGC‑TTTAGACAATAAATTCACCTAAAGTTTTCATAAAACtt                                                                                               >  2:700921/1‑140 (MQ=255)
                                    tCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTc                                                                                             >  2:8727/1‑140 (MQ=255)
                                         cTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGAAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        >  1:57612/1‑140 (MQ=255)
                                              ttgATATCGCGGTGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAGGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            >  2:736925/1‑131 (MQ=255)
                                              ttgATATCGCGGTGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAGGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            <  1:736925/131‑1 (MQ=255)
                                              ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            >  1:959329/1‑131 (MQ=255)
                                              ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            <  2:959329/131‑1 (MQ=255)
                                              ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   <  2:829576/140‑1 (MQ=255)
                                              ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   >  2:307897/1‑140 (MQ=255)
                                              ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   >  2:260617/1‑140 (MQ=255)
                                                gATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGttt                                                                                 <  1:835871/140‑1 (MQ=255)
                                                 atatCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                <  1:307897/140‑1 (MQ=255)
                                                   atCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCaa                                                                                      >  2:416117/1‑132 (MQ=255)
                                                   atCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCaa                                                                                      <  1:416117/132‑1 (MQ=255)
                                                    tCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                >  1:175654/1‑137 (MQ=255)
                                                    tCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                <  2:175654/137‑1 (MQ=255)
                                                     cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        <  2:239526/128‑1 (MQ=255)
                                                     cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        >  1:239526/1‑128 (MQ=255)
                                                     cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGa                                                                            >  1:567073/1‑140 (MQ=255)
                                                        gATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTa                                                                         >  2:675262/1‑140 (MQ=255)
                                                        gATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTa                                                                         >  2:389756/1‑140 (MQ=255)
                                                           ggATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAg                                                                      >  1:274839/1‑140 (MQ=255)
                                                              tAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGa                                                                   <  2:325691/140‑1 (MQ=255)
                                                                       cgcCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGcaacaa                                                          >  2:515223/1‑140 (MQ=255)
                                                                         cccAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATc                                                        >  1:520691/1‑140 (MQ=255)
                                                                                  aTTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAaat                                               <  1:818583/140‑1 (MQ=255)
                                                                                       cGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAAtct                                          <  2:274839/140‑1 (MQ=255)
                                                                                        gACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACa                                                  >  2:542655/1‑131 (MQ=255)
                                                                                        gACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACa                                                  <  1:542655/131‑1 (MQ=255)
                                                                                            gCAAAGCAGTGAGCTCACCGGTAGCACGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATg                                     <  1:675262/140‑1 (MQ=255)
                                                                                                   aGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCt                              <  1:8727/140‑1 (MQ=255)
                                                                                                     tGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCa                            >  1:776899/1‑140 (MQ=255)
                                                                                                            aCCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAAt                     >  2:491509/1‑140 (MQ=255)
                                                                                                                      tGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCa           >  1:516723/1‑140 (MQ=255)
                                                                                                                      tGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCa           >  2:372011/1‑140 (MQ=255)
                                                                                                                        agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  2:951623/1‑140 (MQ=255)
                                                                                                                        agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  1:363090/1‑140 (MQ=255)
                                                                                                                        agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  2:591116/1‑140 (MQ=255)
                                                                                                                          aaaaCTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAAtt       <  1:951623/140‑1 (MQ=255)
                                                                                                                             aCTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACg    <  2:634947/140‑1 (MQ=255)
                                                                                                                              cTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACgg   <  2:76561/140‑1 (MQ=255)
                                                                                                                               tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  1:205492/140‑1 (MQ=255)
                                                                                                                               tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:776899/140‑1 (MQ=255)
                                                                                                                               tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:567073/140‑1 (MQ=255)
                                                                                                                               tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:363090/140‑1 (MQ=255)
                                                                                                                               tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  >  2:205492/1‑140 (MQ=255)
                                                                                                                                 gTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCgcgc                <  1:376941/124‑1 (MQ=255)
                                                                                                                                 gTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCgcgc                >  2:376941/1‑124 (MQ=255)
                                                                                                                                  tgctgc‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  1:449948/137‑1 (MQ=255)
                                                                                                                                  tgctgc‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  >  2:449948/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCTTTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGA  >  NC_000913/4455442‑4455709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A