Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,256	(C)_8→9	pseudogene (3831/5422 nt)	yneO ←	AIDA‑I family autotransporter YneO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,248	1	.	C	95.0%	283.4 / 6.5	81	pseudogene (3839/5422 nt)	yneO	AIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (41/35); total (45/36)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-02

AGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTGG  >  NC_000913/1594111‑1594380

aGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑cc                                                                                                                                     >  1:486369/1‑140 (MQ=255)

  cGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑ccc‑c                                                                                                                                  <  2:709273/140‑1 (MQ=255)

          aGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑ccc‑ccccccacg                                                                                                                          <  1:180959/140‑5 (MQ=255)

            tGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGat                                                                                                                         <  1:307166/140‑1 (MQ=255)

              ccccccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGatat                                                                                                                       <  1:1024714/140‑1 (MQ=255)

              ccccccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGatat                                                                                                                       <  2:151382/140‑1 (MQ=255)

                 cccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcg                                                                                                                    <  2:678840/140‑1 (MQ=255)

                  ccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgt                                                                                                                   <  1:336471/140‑1 (MQ=255)

                    ccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgtcg                                                                                                                 <  1:1083281/140‑1 (MQ=255)

                    ccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgtcg                                                                                                                 >  1:116106/1‑140 (MQ=255)

                    ccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgtcg                                                                                                                 <  2:705032/140‑1 (MQ=255)

                      aGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTa                                                                                                               >  1:731575/1‑140 (MQ=255)

                            aaCGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgtcg                                                                                                                 <  2:529852/132‑1 (MQ=255)

                            aaCGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCcgtcg                                                                                                                 >  1:529852/1‑132 (MQ=255)

                             aCGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGAt                                                                                                        >  2:159350/1‑140 (MQ=255)

                             aCGTTCATATCACCTACAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGAt                                                                                                        >  1:631937/1‑140 (MQ=255)

                                  cATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATAcgcc                                                                                                   >  2:1059771/1‑140 (MQ=255)

                                   atatCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATAcgccg                                                                                                  >  2:515035/1‑140 (MQ=255)

                                        aCCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                             <  2:86950/140‑1 (MQ=255)

                                                 ggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTa                                                                                    >  2:317268/1‑140 (MQ=255)

                                                  gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                   >  2:1060919/1‑140 (MQ=255)

                                                  gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                   >  2:1275588/1‑140 (MQ=255)

                                                  gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                   >  2:508888/1‑140 (MQ=255)

                                                         ttCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAg                                                                            <  1:369535/140‑1 (MQ=255)

                                                            ccGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGAt                                                                         >  2:1118131/1‑140 (MQ=255)

                                                            ccGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGAt                                                                         >  2:84457/1‑140 (MQ=255)

                                                              gACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATcc                                                                       <  1:804054/140‑1 (MQ=255)

                                                                   cacaCCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGt                                                                   >  2:322605/1‑140 (MQ=255)

                                                                      acCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTAccc                                                               >  1:1202954/1‑140 (MQ=255)

                                                                           tGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGttt                                                          >  2:616657/1‑140 (MQ=255)

                                                                               aTGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTc                                                      <  1:698970/140‑1 (MQ=255)

                                                                                     atTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTG‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATcc                                                >  2:1043706/1‑139 (MQ=255)

                                                                                     atTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCa                                                >  2:1032919/1‑140 (MQ=255)

                                                                                      ttAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCac                                               <  1:324507/140‑1 (MQ=255)

                                                                                       tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCac                                               <  1:322605/140‑1 (MQ=255)

                                                                                       tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTTGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                              >  1:1219883/1‑140 (MQ=255)

                                                                                       tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCATATCacc                                              >  2:189162/1‑140 (MQ=255)

                                                                                       tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                              >  1:652409/1‑140 (MQ=255)

                                                                                       tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccg                                             >  1:786378/1‑140 (MQ=255)

                                                                                          tgtgTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                           <  2:83744/140‑1 (MQ=255)

                                                                                          tgtgTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                           <  2:1100374/140‑1 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTTGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                         <  2:1219883/140‑1 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                         >  2:84890/1‑140 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                         >  2:104768/1‑140 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                         >  2:1262311/1‑140 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                         <  2:652409/140‑1 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                           <  2:223974/138‑1 (MQ=255)

                                                                                            tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                           >  1:223974/1‑138 (MQ=255)

                                                                                              ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCCACCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                       >  1:297490/1‑140 (MQ=255)

                                                                                              ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccgc                                      >  2:1210592/1‑140 (MQ=255)

                                                                                              ttGACTTGCC‑‑ATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccgct                                     >  2:1276792/1‑140 (MQ=255)

                                                                                                 aCTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTg                                    >  2:887618/1‑140 (MQ=255)

                                                                                                    tGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATa                                 <  1:84457/140‑1 (MQ=255)

                                                                                                      ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACt                               >  2:1106718/1‑140 (MQ=255)

                                                                                                      ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACt                               >  2:1086105/1‑140 (MQ=255)

                                                                                                      ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACt                               >  1:817710/1‑140 (MQ=255)

                                                                                                         tatTAATGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACtgtg                            <  1:159350/140‑1 (MQ=255)

                                                                                                              aTGGCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCa                       <  1:1032919/140‑1 (MQ=255)

                                                                                                                ggCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                     >  2:144975/1‑140 (MQ=255)

                                                                                                                ggCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                     >  1:609279/1‑140 (MQ=255)

                                                                                                                ggCAGAGTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                     >  2:579556/1‑140 (MQ=255)

                                                                                                                   agagTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                  <  1:1060919/140‑1 (MQ=255)

                                                                                                                   agagTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                  <  1:1118131/140‑1 (MQ=255)

                                                                                                                   agagTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                  <  1:887618/140‑1 (MQ=255)

                                                                                                                   agagTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                  <  1:719317/140‑1 (MQ=255)

                                                                                                                   agagTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGcc                  <  1:84890/140‑1 (MQ=255)

                                                                                                                     agTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                >  2:907822/1‑140 (MQ=255)

                                                                                                                     agTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                >  1:707056/1‑140 (MQ=255)

                                                                                                                     agTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGAGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                >  1:912768/1‑140 (MQ=255)

                                                                                                                     agTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCATACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                >  2:617417/1‑140 (MQ=255)

                                                                                                                     agTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGGCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGAACCCGGGACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                >  2:180434/1‑140 (MQ=255)

                                                                                                                      gTCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAg               >  2:1121828/1‑140 (MQ=255)

                                                                                                                       tCGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGc              >  1:873604/1‑140 (MQ=255)

                                                                                                                        cGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt             <  1:317268/140‑1 (MQ=255)

                                                                                                                        cGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt             <  2:817710/140‑1 (MQ=255)

                                                                                                                        cGGAACCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt             <  1:1276792/140‑1 (MQ=255)

                                                                                                                          gAACCGAAAGCAATTTCCCCC‑CCCCCACGTTATCCGTCGTACCCTGAAACGCCGCGAAATCGTTGTTAAGGGTCAGGATCCCGGTACCCAGTTTTGTCCCATCCCCACTACCGCTGATACTGTGCGTCTTCGCCCAGCTa            >  2:29455/1‑140 (MQ=255)

                                                                                                                          gAACCGAAAGCAATTT‑‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg          <  1:1210592/140‑1 (MQ=255)

                                                                                                                           aaCCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCATATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg          <  1:189162/140‑1 (MQ=255)

                                                                                                                           aaCCGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg          <  1:1262311/140‑1 (MQ=255)

                                                                                                                              cGAAAGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc       <  2:496421/140‑1 (MQ=255)

                                                                                                                                aaaGCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg     >  2:701999/1‑140 (MQ=255)

                                                                                                                                   gCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTgg  <  2:486369/140‑1 (MQ=255)

                                                                                                                                   gCAATTTC‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTgg  <  1:515035/140‑1 (MQ=255)

AGCGACACGCAGTGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑‑CCC‑CCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTGG  >  NC_000913/1594111‑1594380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A