Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,878	T→C	N45S (AAC→AGC)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,878	0	T	C	100.0%	275.2 / NA	73	N45S (AAC→AGC)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/45); total (28/45)

GTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTC  >  NC_000913/2821746‑2822009

gTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATccc                                                                                                                              <  1:510212/140‑1 (MQ=255)

gTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATccc                                                                                                                              >  2:794734/1‑140 (MQ=255)

  aTGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCgg                                                                                                                            >  2:771387/1‑140 (MQ=255)

  aTGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCgg                                                                                                                            <  1:1391753/140‑1 (MQ=255)

    ggTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCg                                                                                                                          >  2:1185791/1‑140 (MQ=255)

        aCGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTgg                                                                                                                      >  1:931614/1‑140 (MQ=255)

          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  2:1282670/1‑140 (MQ=255)

          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  1:983065/1‑140 (MQ=255)

          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCCTGTCGACCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTa                                                                                                                    >  2:1173670/1‑140 (MQ=255)

           cgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTaa                                                                                                                   >  1:1437349/1‑140 (MQ=255)

               gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAaacaa                                                                                                               >  2:1424039/1‑140 (MQ=255)

                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCCCGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGt                                                                                                         <  2:1227568/140‑1 (MQ=255)

                         cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1086487/140‑1 (MQ=255)

                         cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1374714/140‑1 (MQ=255)

                         cGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTggg                                                                                                     <  1:1381409/140‑1 (MQ=255)

                          gTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGt                                                                                                    <  2:66154/140‑1 (MQ=255)

                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGt                                                                                                 <  2:345956/140‑1 (MQ=255)

                               ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTa                                                                                               <  1:896861/140‑1 (MQ=255)

                               ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTCGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTa                                                                                               <  1:775254/140‑1 (MQ=255)

                                 aGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATg                                                                                             <  2:129075/140‑1 (MQ=255)

                                 aGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATg                                                                                             <  2:399197/140‑1 (MQ=255)

                                     cGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGt                                                                                         >  1:148056/1‑140 (MQ=255)

                                      gTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTa                                                                                        >  2:117150/1‑140 (MQ=255)

                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTAcc                                                                                      >  2:1230523/1‑140 (MQ=255)

                                            tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAggg                                                                                  >  2:936499/1‑140 (MQ=255)

                                            tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAggg                                                                                  <  1:718255/140‑1 (MQ=255)

                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAg                                                                                <  1:1176263/140‑1 (MQ=255)

                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAg                                                                                <  1:443227/140‑1 (MQ=255)

                                                 caccCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAgctg                                                                             <  1:143686/140‑1 (MQ=255)

                                                   cccGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAgctgct                                                                           <  2:93020/140‑1 (MQ=255)

                                                    ccGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGctgctg                                                                          >  1:1408441/1‑140 (MQ=255)

                                                        acgcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCaa                                                                      <  1:1282670/140‑1 (MQ=255)

                                                        acgcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCaa                                                                      <  1:1173670/140‑1 (MQ=255)

                                                          gcacgccGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  2:634565/140‑1 (MQ=255)

                                                          gcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  1:216389/140‑1 (MQ=255)

                                                          gcacgcaGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACt                                                                    <  1:794734/140‑1 (MQ=255)

                                                               caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:706444/140‑1 (MQ=255)

                                                               caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:750691/140‑1 (MQ=255)

                                                               caGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTg                                                               <  1:1185791/140‑1 (MQ=255)

                                                                aGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGa                                                              <  2:411394/140‑1 (MQ=255)

                                                                  cGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATg                                                            >  1:1166335/1‑140 (MQ=255)

                                                                      gggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTccc                                                        <  1:115387/140‑1 (MQ=255)

                                                                       ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:1184920/140‑1 (MQ=255)

                                                                       ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:676485/140‑1 (MQ=255)

                                                                       ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:117150/140‑1 (MQ=255)

                                                                       ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  1:637859/140‑1 (MQ=255)

                                                                       ggAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCt                                                       <  2:895654/140‑1 (MQ=255)

                                                                        gAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCtt                                                      >  1:857465/1‑140 (MQ=255)

                                                                            tGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTa                                                  >  2:628683/1‑140 (MQ=255)

                                                                              gTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATg                                                >  2:968706/1‑140 (MQ=255)

                                                                                  cgcgGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACAACCTGATGTCCCTTACTATGGaaa                                            <  2:1149452/140‑1 (MQ=255)

                                                                                   gcgGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGaaaa                                           >  2:1078594/1‑140 (MQ=255)

                                                                                      ggAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGt                                        >  2:588791/1‑140 (MQ=255)

                                                                                      ggAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGt                                        >  1:544866/1‑140 (MQ=255)

                                                                                           aatTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCgaga                                   <  1:710722/140‑1 (MQ=255)

                                                                                             ttACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGaaa                                 <  2:51865/140‑1 (MQ=255)

                                                                                              tACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGG‑ACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACg                               <  2:931614/140‑1 (MQ=255)

                                                                                                 gCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGCACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGcc                             >  2:385236/1‑140 (MQ=255)

                                                                                                     gTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGAc                         >  2:101462/1‑140 (MQ=255)

                                                                                                     gTCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGAc                         >  1:1220444/1‑140 (MQ=255)

                                                                                                      tCGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACg                        <  1:936499/140‑1 (MQ=255)

                                                                                                       cGAGCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACgg                       >  1:1388457/1‑140 (MQ=255)

                                                                                                          gCCCAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtc                    >  1:682670/1‑140 (MQ=255)

                                                                                                           cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  2:983065/140‑1 (MQ=255)

                                                                                                           cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  1:15450/140‑1 (MQ=255)

                                                                                                           cccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtct                   <  2:148056/140‑1 (MQ=255)

                                                                                                            ccAAGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGAtctc                  <  1:667133/140‑1 (MQ=255)

                                                                                                               aGGAACACATCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGc               <  2:1437349/140‑1 (MQ=255)

                                                                                                                     acaTCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGct         >  1:829396/1‑140 (MQ=255)

                                                                                                                      caTCCTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGctc        <  1:1424039/140‑1 (MQ=255)

                                                                                                                          cTTGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGCACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGc    >  2:141064/1‑140 (MQ=255)

                                                                                                                           ttGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCt   <  1:588791/140‑1 (MQ=255)

                                                                                                                           ttGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCt   <  2:857465/140‑1 (MQ=255)

                                                                                                                            tGAACTGGCTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTc  >  1:1517301/1‑140 (MQ=255)

GTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTC  >  NC_000913/2821746‑2822009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A