Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,945	T→C	A334A (GCT→GCC)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,945	0	T	C	100.0%	194.6 / NA	54	A334A (GCT→GCC)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/37); total (17/37)

CGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTG  >  NC_000913/3240810‑3241080

cGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCtctc                                                                                                                                     <  1:1223357/140‑1 (MQ=255)

    tCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCCGCAACCACTATTACCCCGTTTGCGCTGGCTGCGGGTGAAAAGCCGGGGGTTCCGGGCCGGCTGCCCCCCGCGCCGCGGTTGGGCGGGGGGGGCTCTCtgtg                                                                                                                                 >  2:1101462/1‑140 (MQ=255)

                      accaTCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTATGTGTGCCTGTGGCGCATCCgg                                                                                                               <  1:620542/140‑1 (MQ=255)

                        caTCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCg                                                                                                             <  1:1405452/140‑1 (MQ=255)

                            aaTATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGGGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                         <  2:1092659/140‑1 (MQ=255)

                               atGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  1:722799/140‑1 (MQ=255)

                               atGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGCGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  1:1174559/140‑1 (MQ=255)

                               atGGCGGGCGAAGCAATCACTCTTACCGGGTTGACGCTGGTTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCGCGGCGCTGCTGTTGAGCGTGGTGGCCTATCTGTGTGCCTGTGGCGCATCCGGCGTGGCggg                                                                                                      <  2:1027491/140‑1 (MQ=255)

                                tGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggg                                                                                                     <  2:315558/140‑1 (MQ=255)

                                  gcgggcgCAGCAATCACTATTGCCGTGTTGACGCTGGCTGCGGTTAATCCGCGGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggggg                                                                                                   <  2:164403/140‑1 (MQ=255)

                                  gcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCgggggg                                                                                                   <  1:1027598/140‑1 (MQ=255)

                                    gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                 <  1:1044037/140‑1 (MQ=255)

                                    gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                 <  1:157463/140‑1 (MQ=255)

                                       cgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtctc                                                                                              <  1:824728/140‑1 (MQ=255)

                                        gcagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTctct                                                                                             <  1:369028/140‑1 (MQ=255)

                                         cagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctg                                                                                            <  1:397491/140‑1 (MQ=255)

                                                     ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:1423763/140‑1 (MQ=255)

                                                     ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:1439079/140‑1 (MQ=255)

                                                     ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                                <  1:792245/140‑1 (MQ=255)

                                                        ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                             <  1:176204/140‑1 (MQ=255)

                                                         cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                            <  1:1101462/140‑1 (MQ=255)

                                                          gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                           <  1:753461/140‑1 (MQ=255)

                                                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGTCTGCTGCTGGTCCCACCGGcc                                                                          >  2:1431558/1‑140 (MQ=255)

                                                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCCGCTGCCGATCCCACTGGcc                                                                          >  2:421177/1‑140 (MQ=255)

                                                            gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCt                                                                         >  1:347230/1‑140 (MQ=255)

                                                             ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTg                                                                        >  1:365654/1‑140 (MQ=255)

                                                             ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTg                                                                        >  1:274124/1‑140 (MQ=255)

                                                             ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTCCCTCTTATCCCCcctgcctg                                                                        >  2:1055480/1‑140 (MQ=255)

                                                               gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                      >  1:856658/1‑140 (MQ=255)

                                                                       ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                              <  1:1378825/140‑1 (MQ=255)

                                                                        tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                             <  1:1238072/140‑1 (MQ=255)

                                                                          cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGt                                                           <  1:1306783/140‑1 (MQ=255)

                                                                            gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGAt                                                         >  1:1041384/1‑140 (MQ=255)

                                                                                atccGCTGTGTATTCCGGTCGTTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCATCTCTGTGTGCCTGGGGCGCATCCGGGGTGGCGGGGGGGTCTCTGCTGCTGATCCCGCTGGCCTGTAATATGTTCGGTATTTCg                                                     <  2:735897/137‑1 (MQ=255)

                                                                                aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGAGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTCTTGTTCCCACTTTGCTGTAATATTGTCgggtgtttg                                                     >  2:277307/1‑133 (MQ=255)

                                                                                  aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGTCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                   <  1:1091435/140‑1 (MQ=255)

                                                                                  aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                   <  1:220689/140‑1 (MQ=255)

                                                                                       gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGACCCTCCCCCTTAACCCAACGGCCTAGAACATGTTCGGGATTTCGAACGcta                                              >  2:146979/1‑140 (MQ=255)

                                                                                          tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                           <  2:1546144/140‑1 (MQ=255)

                                                                                           aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                          <  1:90197/140‑1 (MQ=255)

                                                                                             tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGGATTTCGAACGATATCGccc                                        >  2:1267874/1‑139 (MQ=255)

                                                                                              ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCCGTAATATGTTCGGGATTTTGAACGATATCGCCAt                                       >  2:1196864/1‑140 (MQ=255)

                                                                                                  tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                   >  1:1236445/1‑140 (MQ=255)

                                                                                                  tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCCGATCCCCCCGGCCCGTAATAAGTTCGGGTTTTCGGACGAGATCCCCCTGCAg                                   >  2:457764/1‑140 (MQ=255)

                                                                                                   cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg                                  <  1:919552/140‑1 (MQ=255)

                                                                                                       cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                              <  1:1431558/140‑1 (MQ=255)

                                                                                                                  gctgctGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt                   <  1:688607/140‑1 (MQ=255)

                                                                                                                      ctgTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTat               <  1:421177/140‑1 (MQ=255)

                                                                                                                         ttGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat            <  2:274124/140‑1 (MQ=255)

                                                                                                                         ttGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat            <  2:365654/140‑1 (MQ=255)

                                                                                                                              cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  1:1196864/140‑1 (MQ=255)

                                                                                                                              cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  2:1236445/140‑1 (MQ=255)

                                                                                                                              cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg       <  2:856658/140‑1 (MQ=255)

                                                                                                                                tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTa     <  2:970339/140‑1 (MQ=255)

                                                                                                                                tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGGCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGTGGGTGCCGGCGGCTTTATCATCGGCgga     >  2:298597/1‑138 (MQ=255)

                                                                                                                                tggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCCGCTGCTTATCCCACTGGCCTGTTAAATGTTTGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGCCCTCGGCTTTCTTATCCGCGTa     >  2:587984/1‑140 (MQ=255)

                                                                                                                                   tggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTTTTCCTGCACATACCTCATGCCAGTAATAAGATAAGCATTTTGAACAAAATTGCCAACCAAGAGGGTGCCGTCGGCTTTATACTCGGCGtcctc  >  2:982708/1‑136 (MQ=255)

CGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTG  >  NC_000913/3240810‑3241080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A