Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	170	48.1	1621790	99.2%	1608815	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,010,569	T→C	V181A (GTG→GCG)	hyuA →	phenylhydantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,010,569	0	T	C	100.0%	155.6 / NA	44	V181A (GTG→GCG)	hyuA	phenylhydantoinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/22); total (22/22)

GAAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTG  >  NC_000913/3010439‑3010705

gaAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGa                                                                                                                                 <  2:91706/140‑1 (MQ=255)

  aaTCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGaaa                                                                                                                               >  2:221821/1‑140 (MQ=255)

  aaTCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGaaa                                                                                                                               >  1:459053/1‑140 (MQ=255)

    tCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAat                                                                                                                             >  1:261563/1‑140 (MQ=255)

         atgatAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGc                                                                                                                        >  2:773783/1‑140 (MQ=255)

         atgatAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGc                                                                                                                        >  2:727603/1‑140 (MQ=255)

            atAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGc                                                                                                                     <  1:681251/140‑1 (MQ=255)

              aGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTa                                                                                                                   <  2:296697/140‑1 (MQ=255)

                              aGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAgcgggc                                                                                                   >  1:405744/1‑140 (MQ=255)

                               gCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTCACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAgcgggcg                                                                                                  <  2:66044/140‑1 (MQ=255)

                                   ttttAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAAt                                                                                              >  1:249985/1‑140 (MQ=255)

                                   ttttAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAAt                                                                                              >  1:594746/1‑140 (MQ=255)

                                             tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcgccgc                                                                                    >  1:446206/1‑140 (MQ=255)

                                             tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcgccgc                                                                                    <  2:405744/140‑1 (MQ=255)

                                             tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcg                                                                                        >  1:797269/1‑136 (MQ=255)

                                             tATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATcg                                                                                        <  2:797269/136‑1 (MQ=255)

                                                     cTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTaa                                                                            >  2:319120/1‑140 (MQ=255)

                                                       aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  2:414175/1‑140 (MQ=255)

                                                       aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  1:191074/1‑140 (MQ=255)

                                                       aTCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCCATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAcc                                                                          >  1:95666/1‑140 (MQ=255)

                                                          aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:727603/140‑1 (MQ=255)

                                                          aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:809656/140‑1 (MQ=255)

                                                          aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  1:85944/140‑1 (MQ=255)

                                                          aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  2:446206/140‑1 (MQ=255)

                                                          aaTACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCCATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcg                                                                       <  2:95666/140‑1 (MQ=255)

                                                               aaaCTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAAccgcgacgcg                                                                  >  2:746147/1‑140 (MQ=255)

                                                                 aCTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCt                                                                >  2:236848/1‑140 (MQ=255)

                                                                  cTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTa                                                               >  1:223999/1‑140 (MQ=255)

                                                                  cTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTa                                                               >  2:658015/1‑140 (MQ=255)

                                                                   tCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTAt                                                              <  1:297113/140‑1 (MQ=255)

                                                                    cAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATc                                                             <  1:39010/140‑1 (MQ=255)

                                                                      aCGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCAt                                                           >  2:502614/1‑140 (MQ=255)

                                                                            aCGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCCATCATGCCTTg                                                     >  1:190971/1‑140 (MQ=255)

                                                                              gAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAg                                                   <  1:66381/140‑1 (MQ=255)

                                                                              gAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAg                                                   <  2:594746/140‑1 (MQ=255)

                                                                                ggTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTc                                                 <  1:221821/140‑1 (MQ=255)

                                                                                               aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCGCCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  2:459053/140‑1 (MQ=255)

                                                                                               aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  2:261563/140‑1 (MQ=255)

                                                                                               aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  <  1:319120/140‑1 (MQ=255)

                                                                                               aCGCCGCTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCg                                  >  2:124419/1‑140 (MQ=255)

                                                                                                     cTTGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCgg                            <  1:785328/140‑1 (MQ=255)

                                                                                                      ttGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGa                           >  2:726173/1‑140 (MQ=255)

                                                                                                      ttGCATGAATCCGGCGCGCTGACCACCGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGa                           <  1:718112/140‑1 (MQ=255)

                                                                                                                               ccGCGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTg  <  1:122879/140‑1 (MQ=255)

GAAATCCCGATGATAGTCGAGGAAGGACTGAGCAGTTTTAAACTCTATTTAACCTATCAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGCTTGCATGAATCCGGCGCGCTGACCACCGTGCACCCGGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGAGTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTG  >  NC_000913/3010439‑3010705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A