breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 8,588 A→G K117K (AAA→AAG talB → transaldolase B
RA 10,933 C→T D142N (GAT→AAT) 
S35L (TCG→TTG) 
yaaW ←
mbiA →
putative enzyme‑specific chaperone YaaW
uncharacterized protein MbiA
RA 19,701 C→T intergenic (+81/+110) nhaR → / ← insB‑1 DNA‑binding transcriptional activator NhaR/IS1 protein InsB
RA 20,770 (A)7→8 intergenic (‑262/+45) insA‑1 ← / ← rpsT IS1 protein InsA/30S ribosomal subunit protein S20
RA 113,502 G→A R20H (CGC→CAC)  guaC → GMP reductase
RA 118,912 A→G A60A (GCA→GCG ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
RA 120,164 A→G intergenic (+29/+14) ampE → / ← aroP protein AmpE/aromatic amino acid:H(+) symporter AroP
RA 130,624 G→A P213S (CCC→TCC)  yacH ← DUF3300 domain‑containing protein YacH
RA 136,457 (T)8→7 coding (8/867 nt) speE ← spermidine synthase
RA 140,964 A→G W88R (TGG→CGG)  gcd ← quinoprotein glucose dehydrogenase
RA 195,129 A→G N76S (AAC→AGC)  ispU → undecaprenyl diphosphate synthase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 262,512 G→A G337D (GGC→GAC)  proA → glutamate‑5‑semialdehyde dehydrogenase
RA 272,726 A→G intergenic (+72/‑121) eyeA → / → ykfC CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC
RA 338,109 C→T G444G (GGC→GGT yahF → putative acyl‑CoA synthetase YahF
RA 363,997 C→T W770* (TGG→TAG)  lacZ ← beta‑galactosidase
RA 372,113 T→C intergenic (+8/‑2) mhpC → / → mhpD 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase/2‑hydroxypentadienoate hydratase
RA 373,630 T→C V237A (GTA→GCA)  mhpF → acetaldehyde dehydrogenase (acylating)
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 383,661 (T)7→8 coding (275/1197 nt) yaiP ← putative glucosyltransferase
RA 388,324 C→T A177V (GCG→GTG)  tauD → taurine dioxygenase
RA 523,138 T→C intergenic (+308/‑123) ybbP → / → rhsD putative ABC transporter membrane subunit YbbP/protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 542,474 A→G N196S (AAC→AGC)  glxK → glycerate 2‑kinase 2
RA 543,951 A→G D32D (GAT→GAC allE ← (S)‑ureidoglycine aminohydrolase
RA 602,098 C→T P47L (CCG→CTG)  pheP → phenylalanine:H(+) symporter PheP
RA 634,668 A→G P361P (CCA→CCG ybdL → methionine‑oxo‑acid transaminase, PLP‑dependent
RA 718,276 C→T G729R (GGG→AGG)  speF ← ornithine decarboxylase, degradative
RA 783,505 G→A A114T (GCG→ACG)  pnuC → nicotinamide riboside transporter
RA 805,840 G→A intergenic (+76/+158) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,853 Δ1 bp intergenic (+89/+145) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,855 T→C intergenic (+91/+143) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,863 A→G intergenic (+99/+135) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 851,235 A→G S455P (TCC→CCC)  opgE ← phosphoethanolamine transferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 945,259 A→G V100A (GTC→GCC)  ycaC ← putative hydrolase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,018,976 (A)8→7 intergenic (+39/+37) matP → / ← ompA macrodomain Ter protein/outer membrane porin A
RA 1,058,514 A→G Y144C (TAC→TGC)  torC → cytochrome c menaquinol dehydrogenase TorC
RA 1,060,660 G→A G469S (GGT→AGT)  torA → trimethylamine N‑oxide reductase 1
RA 1,068,636 T→G T402P (ACG→CCG)  rutG ← pyrimidine:H(+) symporter
RA 1,108,491 T→C T151A (ACG→GCG)  opgC ← protein required for succinyl modification of osmoregulated periplasmic glucans
RA 1,124,696 C→T V211I (GTC→ATC)  mdtH ← multidrug efflux pump MdtH
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,224,789 A→G G191G (GGT→GGC minD ← Z‑ring positioning protein MinD
RA 1,239,202 A→G A108A (GCA→GCG dadX → alanine racemase 2
RA 1,282,055 A→G Y731C (TAC→TGC)  narG → nitrate reductase A subunit alpha
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,346,121 C→T G208S (GGC→AGC)  pdeR ← cyclic di‑GMP phosphodiesterase PdeR
RA 1,365,592 C→T P15S (CCG→TCG)  puuE → 4‑aminobutyrate aminotransferase PuuE
RA 1,399,985 (C)7→8 coding (252/516 nt) ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,421,650 (C)7→8 coding (532/858 nt) ydaU → Rac prophage; DUF1376 domain‑containing protein YdaU
RA 1,423,893 (A)6→5 coding (112/1458 nt) trkG → Rac prophage; K(+) transporter TrkG
RA 1,476,379 A→G G412G (GGA→GGG ydbD → DUF2773 domain‑containing protein YdbD
RA 1,478,403 A→G T60A (ACG→GCG)  ynbB → putative CDP‑diglyceride synthase
RA 1,487,156 A→G intergenic (+193/‑79) hrpA → / → ydcF ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,555,425 T→C D82G (GAC→GGC)  maeA ← malate dehydrogenase, NAD‑requiring
RA 1,575,914 T→C Y569C (TAC→TGC)  yddB ← putative TonB‑dependent receptor
RA 1,583,276 T→C K138E (AAA→GAA)  ydeO ← DNA‑binding transcriptional dual regulator YdeO
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,625,704 C→T M559I (ATG→ATA dcp ← peptidyl‑dipeptidase Dcp
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,701,234 G→A G93D (GGC→GAC)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,712,518 (C)6→7 intergenic (+360/‑251) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,810,080 T→C intergenic (+32/‑131) hxpB → / → ydjM hexitol phosphatase B/putative inner membrane protein regulated by LexA
RA 1,827,021 G→A D413D (GAC→GAT astB ← N‑succinylarginine dihydrolase
RA 1,835,096 T→C W97R (TGG→CGG)  ydjZ → DedA family protein YdjZ
RA 1,835,696 T→C V61A (GTA→GCA)  ynjA → AhpD‑like domain‑containing protein YnjA
RA 1,860,490 T→C K281K (AAA→AAG ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,880,907 (A)5→4 coding (903/924 nt) dmlR ← DNA‑binding transcriptional regulator DmlR
RA 1,882,947 G→A A346T (GCC→ACC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,997,792 T→C N8S (AAC→AGC)  tcyN ← cystine ABC transporter ATP binding subunit
RA 2,106,559 A→G V223A (GTT→GCT)  wbbH ← putative O‑antigen polymerase
RA 2,125,891 A→G V111A (GTA→GCA)  gmm ← GDP‑mannose mannosyl hydrolase
RA 2,143,684 (G)6→7 coding (419/3318 nt) dgcE → putative diguanylate cyclase DgcE
RA 2,156,246 G→A V328V (GTG→GTA mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,020 A→G L62P (CTG→CCG)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
RA 2,214,339 (C)6→5 coding (306/1686 nt) btsS ← high‑affinity pyruvate receptor
RA 2,220,451 +C coding (1539/2298 nt) bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,231,366 A→G S116S (TCA→TCG yohK → PF04712 family membrane protein YohK
RA 2,253,386 T→C T254A (ACC→GCC)  nupX ← putative nucleoside transporter
RA 2,288,561 (C)7→8 pseudogene (354/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,308,123 A→T F165I (TTT→ATT)  yojI ← ABC transporter family protein/microcin J25 efflux protein
RA 2,319,449 T→C N143S (AAC→AGC)  rcsC ← sensory histidine kinase RcsC
RA 2,383,475 T→C F255L (TTC→CTC)  elaD → protease ElaD
RA 2,431,944 T→C L333L (CTA→CTG folC ← bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,520,607 A→G T22T (ACT→ACC gltX ← glutamate‑‑tRNA ligase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,605,253 G→A C148Y (TGC→TAC)  hyfD → hydrogenase 4 component D
RA 2,644,744 G→A H41Y (CAC→TAC)  ndk ← nucleoside diphosphate kinase
RA 2,672,181 T→A P45P (CCT→CCA hcaD → putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit
RA 2,732,474 T→C H567R (CAC→CGC)  clpB ← ClpB chaperone
JC 2,734,543 (C)8→11 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,734,931 T→C N35S (AAC→AGC)  pgeF ← polyphenol oxidase YfiH
RA 2,744,884 A→G V152V (GTT→GTC trmD ← tRNA m(1)G37 methyltransferase
RA 2,775,592 T→C pseudogene (48/272 nt) ypjI → putative UPF0401 protein YpjI
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,813,669 G→A A352T (GCC→ACC)  emrB → multidrug efflux pump membrane subunit EmrB
RA 2,825,534 A→G L15L (TTG→CTG)  mltB ← membrane‑bound lytic murein transglycosylase B
RA 2,838,677 T→C E196E (GAA→GAG ascG ← DNA‑binding transcriptional repressor AscG
RA 2,842,888 T→C E52E (GAA→GAG hycI ← hydrogenase 3 maturation protease
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,900,306 (A)9→8 intergenic (‑33/‑286) ygcW ← / → yqcE putative deoxygluconate dehydrogenase/putative transport protein YqcE
RA 2,910,815 T→C T93A (ACA→GCA)  mazF ← endoribonuclease toxin MazF
RA 2,936,150 T→C G189G (GGT→GGC fucI → L‑fucose isomerase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,974,125 T→C E630E (GAA→GAG aas ← acyltransferase
RA 2,983,421 T→C Q235R (CAG→CGG)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,002,670 G→A L19L (CTG→CTA xdhB → putative xanthine dehydrogenase FAD‑binding subunit XdhB
RA 3,009,586 G→A A275T (GCG→ACG)  ygeY → putative peptidase YgeY
RA 3,010,569 T→C V181A (GTG→GCG)  hyuA → phenylhydantoinase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,124,790 C→T A224T (GCA→ACA)  glcF ← glycolate dehydrogenase, putative iron‑sulfur subunit
RA 3,127,983 T→C L13L (TTA→TTG glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,157,434 A→G D271G (GAC→GGC)  dkgA → methylglyoxal reductase DkgA
RA 3,162,008 T→C Y221C (TAC→TGC)  ftsP ← cell division protein required during stress conditions
RA 3,176,507 A→G G109G (GGT→GGC cpdA ← cAMP phosphodiesterase
RA 3,179,396 A→G T428A (ACG→GCG)  tolC → outer membrane channel TolC
RA 3,221,241 T→C T3A (ACC→GCC)  ygjH ← putative tRNA‑binding protein YgjH
RA 3,243,983 T→C E253E (GAA→GAG uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,269,956 T→C intergenic (+354/+260) yhaC → / ← rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
RA 3,270,143 C→T intergenic (+541/+73) yhaC → / ← rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
RA 3,270,488 (C)7→8 noncoding (105/377 nt) rnpB ← RNase P catalytic RNA component
RA 3,279,087 T→C G58G (GGT→GGC kbaZ → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,370,634 T→C T135A (ACG→GCG)  nanE ← putative N‑acetylmannosamine‑6‑phosphate epimerase
RA 3,375,112 G→A A82T (GCG→ACG)  dcuD → putative transporter DcuD
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,389,224 T→C T38T (ACA→ACG aaeX ← DUF1656 domain‑containing protein AaeX
RA 3,416,904 T→C G624G (GGT→GGC acrF → multidrug efflux pump RND permease AcrF
RA 3,416,925 A→G K631K (AAA→AAG acrF → multidrug efflux pump RND permease AcrF
RA 3,445,283 T→C A105A (GCA→GCG rplR ← 50S ribosomal subunit protein L18
RA 3,454,040 A→G G453G (GGT→GGC gspA ← Type II secretion system protein GspA
RA 3,511,746 (A)8→7 coding (599/906 nt) yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,544,618 G→A A76V (GCC→GTC)  bioH ← pimeloyl‑acyl carrier protein methyl ester esterase
RA 3,559,692 A→G E475E (GAA→GAG rtcR → DNA‑binding transcriptional activator RtcR
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,627,725 T→C N605S (AAC→AGC)  rbbA ← ribosome‑associated ATPase
RA 3,707,077 G→A T210I (ACC→ATC)  dppA ← dipeptide ABC transporter periplasmic binding protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,826 T→C S23P (TCA→CCA)  dinD → DNA damage‑inducible protein D
RA 3,836,534 (C)6→7 intergenic (+218/‑419) selC → / → setC tRNA‑Sec/putative arabinose exporter
RA 3,848,200 A→C L142R (CTG→CGG)  uhpC ← inner membrane protein sensing glucose‑6‑phosphate
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,926,414 A→G G14G (GGT→GGC mioC ← flavoprotein MioC
RA 3,927,168 A→G Y5C (TAC→TGC)  asnA → asparagine synthetase A
RA 4,016,607 T→C G59G (GGT→GGC udp → uridine phosphorylase
RA 4,066,018 A→C F393V (TTC→GTC)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
RA 4,219,075 A→G L160L (CTA→CTG aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,227,425 G→A V1200I (GTT→ATT)  metH → cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,403,047 A→G N337S (AAC→AGC)  hflK → regulator of FtsH protease
RA 4,436,234 A→G V111A (GTC→GCC)  cpdB ← 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,522,512 A→G V528A (GTT→GCT)  yjhG ← KpLE2 phage‑like element; D‑xylonate dehydratase
RA 4,548,234 A→G I165V (ATC→GTC)  fimF → type 1 fimbriae minor subunit FimF
RA 4,565,402 (T)6→5 coding (449/1152 nt) yjiM ← putative dehydratase subunit
RA 4,578,409 A→G L310P (CTA→CCA)  mcrB ← 5‑methylcytosine‑specific restriction enzyme subunit McrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3269984 3270113 130 11 [8] [9] 13 yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
* * ÷ NC_000913 3423783–3424234 3424525–3424239 6–743 11 [10] [8] 14 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3942150–3943392 3945443–3944036 645–3294 11 [10] [7] 11 [rrsC]–[rrlC] [rrsC],gltU,[rrlC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =48 (1.000)24 (0.570) 20/244 0.9 36.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 41 (0.970)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780548 (1.000)15 (0.350) 12/244 2.0 26.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 41 (0.970)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =78 (1.630)74 (1.560) 49/274 0.0 64.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)50 (1.050) 35/276 0.3 92.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =34 (0.710)17 (0.380) 16/258 1.5 34.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 34 (0.760)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269034 (0.710)21 (0.470) 17/258 1.4 39.0% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 34 (0.760)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit