Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 143 68.4 2327250 99.3% 2310959 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,976,831 T→C V78A (GTG→GCG)  galR → DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,8310TC100.0% 185.9 / NA 51V78A (GTG→GCG) galRDNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCC  >  NC_000913/2976698‑2976967
                                                                                                                                     |                                                                                                                                        
ctggctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGc                                                                                                                                    <  1:354342/140‑1 (MQ=255)
  ggctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCgg                                                                                                                                  <  2:723133/140‑1 (MQ=255)
   gctgTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 <  2:418145/140‑1 (MQ=255)
             gTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTg                                                                                                                       <  1:328204/140‑1 (MQ=255)
             gTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTg                                                                                                                       <  1:429612/140‑1 (MQ=255)
              tGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      <  1:746720/140‑1 (MQ=255)
                 aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 >  2:31709/1‑126 (MQ=255)
                 aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGt                                                                                                                                 <  1:31709/126‑1 (MQ=255)
                 aaTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCtt                                                                                                                   >  1:398802/1‑140 (MQ=255)
                  aTGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGTAACGGTCGGTCTGGTCGGTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTa                                                                                                                  >  2:1039272/1‑140 (MQ=255)
                        tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  2:639493/1‑140 (MQ=255)
                        tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  1:840092/1‑140 (MQ=255)
                        tctcTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCacac                                                                                                            >  1:533407/1‑140 (MQ=255)
                             tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      >  1:443741/1‑125 (MQ=255)
                             tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTgg                                                                                                                      <  2:443741/125‑1 (MQ=255)
                             tAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTa                                                                                                       >  1:246182/1‑140 (MQ=255)
                               gCTATCACCCGAACGCCAACGCCCGTGTGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAt                                                                                                     >  2:47496/1‑140 (MQ=255)
                                    cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  1:494007/1‑140 (MQ=255)
                                    cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  1:654436/1‑140 (MQ=255)
                                    cACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAAtttttt                                                                                                >  2:1011634/1‑140 (MQ=255)
                                     aCCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTa                                                                                               <  2:654436/140‑1 (MQ=255)
                                     aCCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTa                                                                                               <  2:676138/140‑1 (MQ=255)
                                      cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTGGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  1:171134/1‑140 (MQ=255)
                                      cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  1:916536/1‑140 (MQ=255)
                                      cccGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTat                                                                                              >  2:1129353/1‑140 (MQ=255)
                                        cGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattg                                                                                            <  1:639493/140‑1 (MQ=255)
                                         gAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattga                                                                                           <  1:635495/140‑1 (MQ=255)
                                          aacgccaacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTattgat                                                                                          >  1:1089845/1‑140 (MQ=255)
                                              ccaacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGc                                                                                      <  2:1089845/140‑1 (MQ=255)
                                               caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     <  1:1011634/140‑1 (MQ=255)
                                               caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     <  2:840092/140‑1 (MQ=255)
                                               caacgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCa                                                                                     >  1:72388/1‑140 (MQ=255)
                                                 acgccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTGGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAAc                                                                                   <  2:171134/140‑1 (MQ=255)
                                                   gccCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACgg                                                                                 <  2:494007/140‑1 (MQ=255)
                                                               gcgcAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGa                                                                     >  1:282611/1‑140 (MQ=255)
                                                                       gACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAag                                                             <  2:72388/140‑1 (MQ=255)
                                                                         ccACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAagag                                                           <  2:398802/140‑1 (MQ=255)
                                                                         ccACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAagag                                                           <  2:533407/140‑1 (MQ=255)
                                                                               aaaCGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAg                                                     >  2:772017/1‑140 (MQ=255)
                                                                                    gtcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCAt                                                >  2:947321/1‑140 (MQ=255)
                                                                                    gtcggtcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCAt                                                >  2:602600/1‑140 (MQ=255)
                                                                                         tcTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGc                                           >  2:864697/1‑140 (MQ=255)
                                                                                                   ggTGATGTTTCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATAGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCg                                 >  2:630213/1‑140 (MQ=255)
                                                                                                            tCCGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCtg                        >  2:712939/1‑140 (MQ=255)
                                                                                                             ccGATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCtgt                       <  1:1014396/140‑1 (MQ=255)
                                                                                                               gATCCGTTTTTCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGtgc                     <  1:772017/140‑1 (MQ=255)
                                                                                                                       tttCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATCGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTgg             >  2:141706/1‑140 (MQ=255)
                                                                                                                       tttCGGTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATCGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTgg             <  1:141706/140‑1 (MQ=255)
                                                                                                                            gTGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGgtcgtc        <  2:916536/140‑1 (MQ=255)
                                                                                                                             tGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTcc       >  1:85451/1‑140 (MQ=255)
                                                                                                                             tGCAATGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTcc       >  1:113801/1‑140 (MQ=255)
                                                                                                                                  tGGCGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGcc  <  2:85451/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CTGGCTGTGCATAGTGCAATGGAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAAACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCGGTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAACGAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCC  >  NC_000913/2976698‑2976967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A