Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	161	50.8	1735388	98.6%	1711092	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,278	A→G	E131E (GAA→GAG)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,278	0	A	G	100.0%	133.9 / NA	39	E131E (GAA→GAG)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/23); total (16/23)

CCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGC  >  NC_000913/1098141‑1098402

ccACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAgta                                                                                                                            >  1:41645/1‑140 (MQ=255)

 cACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAgtat                                                                                                                           >  2:717450/1‑140 (MQ=255)

   cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         <  2:528430/140‑1 (MQ=255)

   cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         <  1:246303/140‑1 (MQ=255)

   cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         >  1:254490/1‑140 (MQ=255)

   cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGAGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTatca                                                                                                                         >  2:232808/1‑140 (MQ=255)

         cGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGa                                                                                                                   <  2:179593/140‑1 (MQ=255)

          gCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGaa                                                                                                                  <  1:752429/140‑1 (MQ=255)

              ggAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGAtt                                                                                                              >  2:646700/1‑140 (MQ=255)

                aaGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGAtttt                                                                                                            <  2:595015/140‑1 (MQ=255)

                    tACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCa                                                                                                        <  2:41645/140‑1 (MQ=255)

                         gTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGc                                                                                                   >  2:130138/1‑140 (MQ=255)

                              ggCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCAtttt                                                                                              >  1:271602/1‑140 (MQ=255)

                              ggCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCAtttt                                                                                              >  2:112126/1‑140 (MQ=255)

                                 gAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTggg                                                                                           <  2:271602/140‑1 (MQ=255)

                                 gAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTggg                                                                                           <  2:254490/140‑1 (MQ=255)

                                  aGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgc                                                                                          <  1:130138/140‑1 (MQ=255)

                                   gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         >  1:819231/1‑140 (MQ=255)

                                   gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         <  1:717450/140‑1 (MQ=255)

                                   gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcg                                                                                         >  2:480537/1‑140 (MQ=255)

                                    cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTAc                                                                                                          <  2:540656/122‑1 (MQ=255)

                                    cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTAc                                                                                                          >  1:540656/1‑122 (MQ=255)

                                    cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcgc                                                                                        >  1:533920/1‑140 (MQ=255)

                                    cAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGgcgc                                                                                        >  2:623090/1‑140 (MQ=255)

                                             gAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACt                                                                               <  1:623090/140‑1 (MQ=255)

                                                  tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 >  2:141454/1‑133 (MQ=255)

                                                  tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 <  1:19455/133‑1 (MQ=255)

                                                  tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 >  2:19455/1‑133 (MQ=255)

                                                  tGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTa                                                                                 <  1:141454/133‑1 (MQ=255)

                                                            cagGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGc                                                                <  2:533920/140‑1 (MQ=255)

                                                             agGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCt                                                               <  1:696060/140‑1 (MQ=255)

                                                                          gTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCaaa                                                  >  2:833435/1‑140 (MQ=255)

                                                                          gTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCaaa                                                  >  2:101844/1‑140 (MQ=255)

                                                                              cGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTg                                              <  1:232808/140‑1 (MQ=255)

                                                                                                cGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTg                            <  1:846709/140‑1 (MQ=255)

                                                                                                         cAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCg                   <  1:833435/140‑1 (MQ=255)

                                                                                                                gTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGt            <  2:819231/140‑1 (MQ=255)

                                                                                                                gTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCCCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGt            <  1:197618/140‑1 (MQ=255)

                                                                                                                          gCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  <  1:101844/140‑1 (MQ=255)

                                                                                                                                  tGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  >  2:485763/1‑132 (MQ=255)

                                                                                                                                  tGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGc  <  1:485763/132‑1 (MQ=255)

CCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGC  >  NC_000913/1098141‑1098402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A