Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F66 I0 R2 122 57.2 2468826 97.5% 2407105 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,743 C→T 100% M1M (ATG→ATA) † yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,7430CT100.0% 182.9 / ‑6.7 55M1M (ATG→ATA) †yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (21/32);  minor base A (0/1);  total (22/33)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGT  >  NC_000913/4633609‑4633866
                                                                                                                                      |                                                                                                                           
aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAAt                                                                                                                        <  1:136482/140‑1 (MQ=255)
aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAAt                                                                                                                        <  2:578768/140‑1 (MQ=255)
     cTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTACGCCGAAGATCTCGTGAAATGCCTGCAGTATGGCCTGTATTTTAGCGGGATTGGTGGTCGCACAGACAACTGGGTGTATAATCAGCa                                                                                                                   >  2:478621/1‑140 (MQ=255)
           cGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTc                                                                                                             >  2:666977/1‑140 (MQ=255)
           cGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTc                                                                                                             >  2:665568/1‑140 (MQ=255)
             gTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAg                                                                                                           >  2:851404/1‑140 (MQ=255)
                  ccGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                                                                                                      >  1:533324/1‑140 (MQ=255)
                     ctctCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGTAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                   <  2:422307/140‑1 (MQ=255)
                     ctctCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                   <  2:956503/140‑1 (MQ=255)
                     ctctCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                   <  1:665486/140‑1 (MQ=255)
                     ctctCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                   <  1:470675/140‑1 (MQ=255)
                     ctctCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                   >  2:843600/1‑140 (MQ=255)
                        tcGACGGCACCGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGAGTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGt                                                                                                >  2:132742/1‑140 (MQ=255)
                          gACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTa                                                                                              <  1:132742/140‑1 (MQ=255)
                          gACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTa                                                                                              <  1:737634/140‑1 (MQ=255)
                                  cGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta                                                                                      >  1:479642/1‑140 (MQ=255)
                                    gttGCAATATGGCAGGATCCTTCGCCGAAGATATCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGtata                                                                                    <  2:332198/138‑1 (MQ=255)
                                            atGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCCGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaa                                                                            <  2:592686/140‑1 (MQ=255)
                                            atGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaa                                                                            <  2:1065771/140‑1 (MQ=255)
                                            atGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAAATAACGTTACAGCAGTATACGGaaaaa                                                                            <  2:1199585/140‑1 (MQ=255)
                                               gCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg                                                                         <  2:87528/140‑1 (MQ=255)
                                               gCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTGGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg                                                                         <  2:311336/140‑1 (MQ=255)
                                                 aGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCa                                                                       <  2:411728/140‑1 (MQ=255)
                                                   gATCCTTCGCCGAAGATCTCGTTAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATg                                                                     >  1:217739/1‑140 (MQ=255)
                                                   gATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATg                                                                     >  2:442816/1‑140 (MQ=255)
                                                   gATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATg                                                                     >  1:949460/1‑140 (MQ=255)
                                                   gATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATg                                                                     >  1:951854/1‑140 (MQ=255)
                                                      ccTTCGCCGAAGATCTCGTGATATGACTGAAGTATTGAATGAATTTTAGCTGGATTGGTGGTCGCACAGACAACTTGGTGTTTAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTa                                                                  <  2:739062/140‑1 (MQ=255)
                                                      ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTa                                                                  <  1:865467/140‑1 (MQ=255)
                                                      ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTa                                                                  <  1:864461/140‑1 (MQ=255)
                                                      ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTa                                                                  <  2:738627/140‑1 (MQ=255)
                                                          cGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAgg                                                              <  2:1052878/140‑1 (MQ=255)
                                                           gCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGt                                                             <  1:340349/140‑1 (MQ=255)
                                                             cGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGtat                                                           >  2:715322/1‑140 (MQ=255)
                                                                aGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTAGAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAcc                                                        >  1:456029/1‑140 (MQ=255)
                                                                        tGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGc                                                <  1:440451/140‑1 (MQ=255)
                                                                          aaaTGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCa                                              <  1:830229/140‑1 (MQ=255)
                                                                          aaaTGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCa                                              <  1:90267/140‑1 (MQ=255)
                                                                                  gCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGaaa                                      >  1:772190/1‑140 (MQ=255)
                                                                                  gCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGaaa                                      >  1:1075280/1‑140 (MQ=255)
                                                                                    tgAATGGCCTGTATTTTATCGGGATTGGTGGTCTCAGAGACAACTTTGTTTATAATCAGCTTTAGTCAGATTATTAACGTTACAGCTGTATACGTAAAAAAAGCATGTTACAGGTTTTCCTAGTCCGCCACGGTGAAACg                                    <  1:872942/139‑1 (MQ=255)
                                                                                    aGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTTTACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACg                                    <  1:164665/140‑1 (MQ=255)
                                                                                      aaTGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCa                                  <  2:237020/140‑1 (MQ=255)
                                                                                        tGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGt                                >  2:589248/1‑140 (MQ=255)
                                                                                           ccagAATTTTAGCGGCATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCAGCCACGGTGAAACGCAGTGGa                             >  2:772722/4‑140 (MQ=255)
                                                                                                   ttAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAg                     <  2:217739/140‑1 (MQ=255)
                                                                                                    tAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGc                    >  1:531540/1‑140 (MQ=255)
                                                                                                      gCGGGATTGCTGGTCGCACAGACAACTTGGCGAAAAATCAGCATTACTCCGACAATACACGATACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGAGAAACGCAGTGGAACGCCGAGCGa                  <  2:189697/140‑1 (MQ=255)
                                                                                                      gCGGCATAGGTGCTCGCCCAGACAAATTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGa                  <  2:149562/140‑1 (MQ=255)
                                                                                                        gggATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACg                <  1:1095776/140‑1 (MQ=255)
                                                                                                         ggATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAACCATGTTACCGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGAcat               >  2:144275/1‑138 (MQ=255)
                                                                                                             tggtggTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc           <  2:768775/140‑1 (MQ=255)
                                                                                                                  gtCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGc      <  2:679983/140‑1 (MQ=255)
                                                                                                                      cacaGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGt  >  1:417347/1‑140 (MQ=255)
                                                                                                                      cacaGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGt  >  1:419735/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                           
AAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGT  >  NC_000913/4633609‑4633866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A