Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F46 I0 R1
|
166 |
60.6 |
2059618 |
97.1% |
1999889 |
148.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,633,743 |
C→T |
38.1% |
M1M (ATG→ATA) † |
yjjX ← |
non‑canonical purine NTP phosphatase, ITPase/XTPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,633,743 | 0 | C | T | 38.1%
| 35.2
/ 47.8
| 48 | M1M (ATG→ATA) † | yjjX | non‑canonical purine NTP phosphatase, ITPase/XTPase |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base T (10/8); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 7.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 |
CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC > NC_000913/4633598‑4633882
|
ccTCCCTGCCCACCGGCTTTTCCGGTCCACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa < 2:844922/149‑1 (MQ=255)
tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATCGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt < 1:107278/149‑1 (MQ=255)
aaaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTa > 2:830031/1‑149 (MQ=255)
aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTAc < 2:512922/149‑1 (MQ=255)
gagCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCGTGCTGAATGGCCTGCATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGGGTATAATCAGCATTACTc > 1:216635/3‑149 (MQ=255)
ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCTAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa < 1:300343/149‑1 (MQ=255)
ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa > 1:528806/1‑149 (MQ=255)
cGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTAca < 1:346965/149‑1 (MQ=255)
cGCTCTCGACGGCAACGGATGCAATAAGGCAGGATCCTTCGCCGAAGATCTCGTGAAAAGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGCCAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAca > 1:637735/1‑149 (MQ=255)
ggCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCCGCATTACTCAGAAAATTAACGTTACAGCAGTATACg > 2:870719/1‑149 (MQ=255)
gCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACgg > 1:382042/1‑149 (MQ=255)
cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt < 2:959324/134‑1 (MQ=255)
cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt > 1:959324/1‑134 (MQ=255)
aTGCAATATTGAAGGAAGATTCGCCGTAGATATGGTGAATTGCCTGATGATTGGGCTGAATTTTAGCGTGATTGGTGGTCGCAGATACAACTTGGTGAATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaaaa < 2:944157/149‑1 (MQ=255)
tGCAATATGGCAGGATCCTTCTCCGAAGATCTCGAGAAATGCCTGCAGAATGGCCTGAATTTTAGCGTTATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg < 1:380515/149‑1 (MQ=255)
gCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGc < 2:616641/149‑1 (MQ=255)
aaTATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCAt > 1:517925/1‑149 (MQ=255)
tatGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGt > 1:73781/1‑149 (MQ=255)
aTCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATTGCCTGAATTTTAGCGGGTTTGGTGGGCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCATTATACGGAAAAAAAGCATGTTACAGGtat < 2:456848/149‑1 (MQ=255)
ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACTGAAAAAAAGCATGTTACAGGTATAc < 1:800674/149‑1 (MQ=255)
cTTCGCCGAAGATCTCGTGAAATTCCTGCAGAATGGCCTGAATAATAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGTTATAcc > 1:682706/1‑149 (MQ=255)
ttCGCCGAAGATCTCGTGAAATGCCTGCAGAATGTCCTGAAATTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATCTTACAGGAATAcat > 1:1005338/1‑147 (MQ=255)
tCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTa > 1:924622/1‑149 (MQ=255)
cGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGGTGTAGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAg < 2:246925/149‑1 (MQ=255)
cGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTcc > 2:575446/1‑149 (MQ=255)
aGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGcc < 2:447466/149‑1 (MQ=255)
aTCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCAc > 1:3018/1‑149 (MQ=255)
tctcGTGAAATGCCTGCAGAATGGCATGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAGAAAAAGCATGTTACAGGTATACCTAGTCCGCCACg < 1:860212/149‑1 (MQ=255)
tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt > 1:794410/1‑149 (MQ=255)
tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt < 1:455040/149‑1 (MQ=255)
gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa < 1:878237/149‑1 (MQ=255)
gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa < 1:656158/149‑1 (MQ=255)
gAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTAGCACATAAAACTTTGTTCATATTCAGCATTACTGAGAAACTTAACTGTACAGGAGTATACGGATAAAAAGCATGTTACAGGTATACCTAGTACGCCTCGGTGaaa < 2:254931/149‑1 (MQ=255)
cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg > 1:141348/1‑149 (MQ=255)
cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg > 1:236988/1‑149 (MQ=255)
cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAATAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACGGTGAAAAGCactt > 1:586356/1‑146 (MQ=255)
aGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAAc < 1:830031/149‑1 (MQ=255)
gAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTACCGTTACAGCAGTATACGGAAACACAGCATGTTACAGCTATACCTAGTCCGCCACGGGGAACCGCAGTGGACCg > 1:334506/1‑149 (MQ=255)
tGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCTTTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACTGAGAAACGCAGTGGACCGCCGAGCGa > 2:260110/1‑149 (MQ=255)
taaTTTTCGCTGGATTGTTGGGTTAACAGAGAAATTGGTGTATCATGAGCTTATCTCAGAAAATTAACGTTACAGAAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCAGCCACGTTGAAACGCAGTGTAACGCCGAGCGAc < 2:965112/148‑1 (MQ=255)
tAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc > 1:564756/1‑149 (MQ=255)
tAGCGGGATAGGTGGTCGCACAGAAAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc < 1:414317/149‑1 (MQ=255)
gCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCAATACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGATACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAg < 2:528806/149‑1 (MQ=255)
gggATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATCCAggg < 1:870719/149‑1 (MQ=255)
ttGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAg > 2:956506/1‑149 (MQ=255)
ggtggtCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTc < 1:359482/149‑1 (MQ=255)
aGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGATACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGc > 2:207487/1‑149 (MQ=255)
aaCTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTCAAACGCAGTGTAACGCAGAGCGACGTATTCAGCGCCAGTCTGACAGCCCGCTGAc > 1:11482/1‑149 (MQ=255)
|
CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC > NC_000913/4633598‑4633882
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
