breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 10,659 C→T 6.2% S233N (AGC→AAC)  yaaW ← UPF0174 family protein
RA 59,625 G→A 7.3% intergenic (+346/+62) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 59,626 A→G 7.7% intergenic (+347/+61) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 59,627 C→T 7.4% intergenic (+348/+60) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 59,628 T→C 7.6% intergenic (+349/+59) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 78,576 G→A 6.8% G319D (GGC→GAC)  setA → broad specificity sugar efflux system
RA 111,016 T→C 5.4% intergenic (+32/‑28) secA → / → mutT preprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 125,817 A→G 11.0% K41K (AAA→AAG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,060 A→G 5.2% E122E (GAA→GAG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,093 T→C 11.1% A133A (GCT→GCC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,126 G→A 11.1% K144K (AAG→AAA aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 163,417 A→T 10.7% K438I (AAA→ATA) ‡ hrpB → putative ATP‑dependent helicase
RA 163,418 A→T 9.6% K438N (AAA→AAT) ‡ hrpB → putative ATP‑dependent helicase
RA 163,419 A→T 9.9% I439F (ATT→TTT)  hrpB → putative ATP‑dependent helicase
RA 201,032 G→A 6.2% G21D (GGC→GAC)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
RA 256,915 C→T 5.1% T130M (ACG→ATG)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,926 A→G 5.5% I134V (ATT→GTT)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 464,354 C→A 8.1% intergenic (+46/‑48) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,356 A→T 9.3% intergenic (+48/‑46) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,358 T→G 11.8% intergenic (+50/‑44) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 475,971 C→G 10.0% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 9.8% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 525,588 A→G 5.1% K776K (AAA→AAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 543,748 G→C 6.9% T100R (ACA→AGA)  allE ← S‑ureidoglycine aminohydrolase
RA 659,204 C→T 7.1% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 7.3% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 8.9% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 7.1% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 676,686 T→A 7.5% intergenic (‑139/‑25) ybeQ ← / → ybeR Sel1 family TPR‑like repeat protein/uncharacterized protein
RA 710,170 C→T 6.5% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 6.7% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 794,939 T→C 8.7% intergenic (+17/‑150) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
RA 808,205 A→C 22.1% R351R (CGT→CGG bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent
RA 951,030 G→A 5.5% D17D (GAC→GAT pflA ← pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase
RA 1,062,893 A→G 10.6% S295P (TCG→CCG)  cbpA ← DnaK co‑chaperone; curved DNA‑binding protein
RA 1,129,394 G→C 7.9% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL
RA 1,141,150 G→A 6.4% intergenic (+164/+32) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,141,151 T→C 7.0% intergenic (+165/+31) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,141,153 A→G 6.5% intergenic (+167/+29) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,243,335 C→T 6.4% V52V (GTC→GTT emtA → lytic murein endotransglycosylase E
RA 1,449,799 C→G 9.0% T517T (ACG→ACC tynA ← tyramine oxidase, copper‑requiring
RA 1,449,802 A→G 8.5% A516A (GCT→GCC) ‡ tynA ← tyramine oxidase, copper‑requiring
RA 1,449,803 G→C 8.8% A516G (GCT→GGT) ‡ tynA ← tyramine oxidase, copper‑requiring
RA 1,449,804 C→T 9.0% A516T (GCT→ACT) ‡ tynA ← tyramine oxidase, copper‑requiring
RA 1,449,807 C→G 8.8% G515R (GGT→CGT)  tynA ← tyramine oxidase, copper‑requiring
RA 1,460,565 G→A 6.2% G32D (GGC→GAC)  paaI → hydroxyphenylacetyl‑CoA thioesterase
RA 1,466,144 G→T 10.7% pseudogene (753/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,258 G→C 13.4% pseudogene (867/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,575,215 C→T 5.1% intergenic (‑13/+32) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
RA 1,575,216 G→A 5.2% intergenic (‑14/+31) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
RA 1,575,221 T→C 5.4% intergenic (‑19/+26) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
RA 1,596,499 C→A 9.0% pseudogene (1489/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,602,990 Δ1 bp 8.3% coding (1501/1536 nt) lsrA → autoinducer 2 import ATP‑binding protein
RA 1,642,370 C→G 6.8% intergenic (+303/+119) cspF → / ← quuQ Qin prophage; cold shock protein/Qin prophage; putative antitermination protein Q
RA 1,704,723 T→C 5.8% intergenic (+47/‑226) blr → / → cnu beta‑lactam resistance membrane protein; divisome‑associated protein/nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor
RA 1,784,808 C→T 7.0% G769R (GGG→AGG)  ppsA ← phosphoenolpyruvate synthase
RA 1,823,335 G→C 11.1% intergenic (+50/‑180) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,823,338 G→C 11.5% intergenic (+53/‑177) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,864,768 T→C 6.4% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,904,041 A→T 5.8% A49A (GCA→GCT manZ → mannose‑specific enzyme IID component of PTS
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,982,500 T→C 6.5% intergenic (‑113/+54) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,982,501 T→C 6.6% intergenic (‑114/+53) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,982,505 G→A 6.1% intergenic (‑118/+49) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,992,083 G→C 5.2% noncoding (35/76 nt) glyW ← tRNA‑Gly
RA 2,015,192 A→G 8.3% S105G (AGC→GGC)  fliG → flagellar motor switching and energizing component
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,044,721 G→A 8.1% intergenic (+97/‑217) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,044,725 T→A 8.6% intergenic (+101/‑213) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,044,727 T→A 7.7% intergenic (+103/‑211) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,044,729 T→A 7.6% intergenic (+105/‑209) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,044,733 T→C 7.8% intergenic (+109/‑205) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,045,793 C→T 9.1% L286L (CTG→TTG)  yeeJ → putative adhesin
RA 2,059,946 T→A 12.3% intergenic (+20/+18) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
RA 2,059,948 T→A 11.4% intergenic (+22/+16) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
RA 2,059,950 T→A 12.9% intergenic (+24/+14) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
RA 2,139,716 T→G 6.8% intergenic (+231/+43) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,139,718 T→A 5.9% intergenic (+233/+41) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,139,720 C→A 6.5% intergenic (+235/+39) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,139,726 T→G 7.3% intergenic (+241/+33) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,139,727 T→C 5.8% intergenic (+242/+32) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,231,175 G→A 8.1% D53N (GAC→AAC)  yohK → LrgB family inner membrane protein
RA 2,310,322 A→T 9.9% V28V (GTT→GTA ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,323,581 C→T 6.4% R45R (CGC→CGT atoD → acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,352,779 G→A 6.1% G45S (GGT→AGT)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
RA 2,357,025 A→G 9.6% N41S (AAC→AGC) ‡ yfaD → transposase_31 family protein
RA 2,357,026 C→G 8.5% N41K (AAC→AAG) ‡ yfaD → transposase_31 family protein
RA 2,357,027 C→T 9.2% L42L (CTG→TTG)  yfaD → transposase_31 family protein
RA 2,357,034 C→G 8.6% T44S (ACC→AGC)  yfaD → transposase_31 family protein
RA 2,357,665 G→A 9.1% G254G (GGG→GGA yfaD → transposase_31 family protein
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,432,985 A→G 5.9% intergenic (‑43/+27) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
RA 2,432,990 G→C 6.6% intergenic (‑48/+22) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
RA 2,529,856 T→C 5.5% K107K (AAA→AAG ligA ← DNA ligase, NAD(+)‑dependent
RA 2,536,370 C→G 7.9% intergenic (+27/+16) crr → / ← pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,536,373 C→G 6.7% intergenic (+30/+13) crr → / ← pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,564,635 G→C 6.6% R38P (CGC→CCC)  yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,564,640 G→C 6.9% G40R (GGA→CGA)  yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,597,699 G→T 7.4% intergenic (‑81/+132) purC ← / ← bamC phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein
RA 2,669,275 C→A 13.0% L82I (CTC→ATC)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit
RA 2,801,056 A→T 11.5% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin
RA 2,851,603 C→T 7.4% S201S (AGC→AGT hypB → GTP hydrolase involved in nickel liganding into hydrogenases
RA 2,904,212 G→A 88.9% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,906,608 G→C 5.1% intergenic (+25/+35) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,906,609 G→C 5.1% intergenic (+26/+34) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,917,910 T→C 6.0% intergenic (+97/+135) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,976,320 T→G 6.9% noncoding (66/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
RA 2,976,321 C→A 8.3% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
RA 2,979,199 C→A 9.3% P60H (CCT→CAT)  lysR → transcriptional activator of lysA; autorepressor
RA 3,020,821 A→G 6.1% S94S (TCA→TCG ygfM → putative oxidoreductase
RA 3,156,591 T→G 7.6% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,171,852 C→T 6.3% intergenic (+19/+27) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,857 A→T 6.5% intergenic (+24/+22) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,858 A→T 6.5% intergenic (+25/+21) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,863 A→G 6.4% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,194,875 T→C 11.8% intergenic (‑10/+224) sibD ← / ← sibE sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein
RA 3,195,148 A→C 8.8% noncoding (93/142 nt)
K3T (AAG→ACG) 
sibE ←
ibsE →
sRNA antisense regulator of toxic IbsE protein
toxic membrane protein
RA 3,195,151 T→G 7.1% noncoding (90/142 nt)
L4R (CTC→CGC) 
sibE ←
ibsE →
sRNA antisense regulator of toxic IbsE protein
toxic membrane protein
RA 3,195,153 G→T 7.0% noncoding (88/142 nt)
V5F (GTC→TTC) 
sibE ←
ibsE →
sRNA antisense regulator of toxic IbsE protein
toxic membrane protein
RA 3,263,892 A→G 5.2% W376R (TGG→CGG)  tdcC ← L‑threonine/L‑serine transporter
RA 3,269,917 T→C 7.9% intergenic (+315/+299) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,440,923 C→A 18.7% G36V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit
RA 3,441,061 G→C 5.8% S205S (TCC→TCG rpsD ← 30S ribosomal subunit protein S4
RA 3,448,276 T→A 5.2% intergenic (‑127/+38) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
RA 3,505,096 C→T 7.5% A309T (GCA→ACA)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,623,820 C→T 5.5% intergenic (+38/‑67) yhhH → / → yrhC putative NTF2 fold immunity protein for polymorphic toxin RhsB/pseudogene fragment
RA 3,689,724 G→C 6.9% P180A (CCC→GCC)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,689,728 A→T 7.0% F178L (TTT→TTA) ‡ bcsZ ← endo‑1,4‑D‑glucanase
RA 3,689,729 A→T 6.8% F178Y (TTT→TAT) ‡ bcsZ ← endo‑1,4‑D‑glucanase
RA 3,689,733 G→C 6.4% R177G (CGT→GGT)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,732,136 A→C 5.6% intergenic (+13/‑65) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
RA 3,817,940 T→A 5.5% F61I (TTC→ATC)  dinD → DNA damage‑inducible protein
RA 3,817,948 G→T 5.1% E63D (GAG→GAT dinD → DNA damage‑inducible protein
RA 3,817,949 A→C 5.6% I64L (ATC→CTC)  dinD → DNA damage‑inducible protein
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,891,506 C→G 6.2% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,965,670 C→T 5.7% intergenic (‑40/‑91) rhlB ← / → trxA ATP‑dependent RNA helicase/thioredoxin 1
RA 4,050,225 A→T 7.7% L181M (TTG→ATG)  yihA ← cell division GTP‑binding protein
RA 4,051,262 A→C 7.2% noncoding (227/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,060,261 G→T 7.0% intergenic (+31/‑186) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,060,264 A→C 8.7% intergenic (+34/‑183) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,155,881 C→T 5.2% A294V (GCC→GTC)  argC → N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
RA 4,296,060 C→T 42.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,072 T→G 8.8% intergenic (‑331/+327) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,332,871 A→C 13.3% S134R (AGT→AGG basS ← sensory histidine kinase in two‑component regulatory system with BasR
RA 4,353,170 G→A 5.3% intergenic (+89/+30) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,353,171 C→G 5.9% intergenic (+90/+29) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,353,172 C→G 5.3% intergenic (+91/+28) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,353,173 T→C 6.2% intergenic (+92/+27) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,362,581 C→A 37.3% noncoding (46/76 nt) pheU ← tRNA‑Phe
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,415,525 G→T 11.3% E417D (GAG→GAT aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,448,424 T→A 5.8% intergenic (+189/‑23) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
RA 4,470,952 T→C 5.8% intergenic (‑39/+34) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,470,958 G→T 7.3% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,554,516 A→G 6.4% intergenic (+155/‑60) uxuB → / → uxuR D‑mannonate oxidoreductase, NAD‑dependent/fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
RA 4,612,329 A→G 8.8% intergenic (+40/‑82) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase
RA 4,612,330 G→C 8.5% intergenic (+41/‑81) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase
RA 4,612,331 C→T 8.4% intergenic (+42/‑80) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase
RA 4,633,743 C→T 38.1% M1M (ATG→ATA) † yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772980 774221 1242 21 [19] [19] 20 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037699 1039310 1612 20 [18] [19] 21 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 3423733–3424234 3424549–3424238 5–817 20 [19] [18] 20 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)4 (0.070) 4/262 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 = 274334NA (NA)4 (0.070) 3/264 NT NA noncoding (816/1195 nt) IS5 repeat region
?NC_000913 = 274338 NA (NA)noncoding (812/1195 nt) IS5 repeat region
* ? NC_000913 274347 =NA (NA)3 (0.050) 3/262 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)3 (0.050) 3/258 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 315646NA (NA)4 (0.070) 4/266 NT NA noncoding (418/1255 nt) IS3 repeat region
?NC_000913 = 315665 NA (NA)noncoding (437/1255 nt) IS3 repeat region
* ? NC_000913 = 37783455 (0.930)3 (0.050) 3/264 NT 5.2% coding (535/834 nt) frmB S‑formylglutathione hydrolase
?NC_000913 = 377846 56 (0.990)coding (523/834 nt) frmB S‑formylglutathione hydrolase
* ? NC_000913 381663 =NA (NA)3 (0.050) 3/262 NT NA noncoding (404/1331 nt) IS2 repeat region
?NC_000913 381703 = NA (NA)noncoding (444/1331 nt) IS2 repeat region
* ? NC_000913 396419 =58 (0.980)4 (0.070) 4/268 NT 6.8% intergenic (‑132/‑220) ampH/sbmA D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase/peptide antibiotic transporter
?NC_000913 396441 = 54 (0.940)intergenic (‑154/‑198) ampH/sbmA D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase/peptide antibiotic transporter
* ? NC_000913 = 41513655 (0.930)4 (0.070) 4/268 NT 7.3% coding (618/3147 nt) sbcC exonuclease, dsDNA, ATP‑dependent
?NC_000913 = 415165 48 (0.830)coding (589/3147 nt) sbcC exonuclease, dsDNA, ATP‑dependent
* ? NC_000913 511333 =51 (0.860)3 (0.050) 3/266 NT 5.9% coding (47/2505 nt) copA copper transporter
?NC_000913 511361 = 46 (0.810)coding (19/2505 nt) copA copper transporter
* ? NC_000913 = 63007941 (0.690)4 (0.070) 4/262 NT 9.9% coding (186/2106 nt) cstA carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
?NC_000913 = 630098 34 (0.600)coding (205/2106 nt) cstA carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
* ? NC_000913 = 64506860 (1.010)4 (0.070) 3/254 NT 6.5% intergenic (‑65/+49) rna/citT ribonuclease I/citrate/succinate antiporter; citrate carrier
?NC_000913 = 645074 59 (1.080)intergenic (‑71/+43) rna/citT ribonuclease I/citrate/succinate antiporter; citrate carrier
* ? NC_000913 = 730045NA (NA)3 (0.050) 3/266 NT NA coding (463/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 730067 NA (NA)coding (485/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 944904 =58 (0.980)4 (0.070) 4/260 NT 6.9% intergenic (+8/+27) dmsC/ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
?NC_000913 944926 = 53 (0.950)intergenic (+30/+5) dmsC/ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
* ? NC_000913 1207790 =11 (0.190)15 (0.290) 14/244 NT 52.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.320)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780512 (0.200)20 (0.380) 17/244 NT 59.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.320)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994981 (0.020)75 (1.300) 62/268 NT 99.4% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1360160 =59 (1.000)4 (0.070) 4/270 NT 6.5% coding (749/1419 nt) puuA glutamate‑‑putrescine ligase
?NC_000913 1360180 = 57 (0.980)coding (729/1419 nt) puuA glutamate‑‑putrescine ligase
* ? NC_000913 1825065 =68 (1.150)4 (0.070) 4/262 NT 5.8% intergenic (‑128/+75) ves/spy cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
?NC_000913 1825118 = 65 (1.160)intergenic (‑181/+22) ves/spy cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
* ? NC_000913 = 182622456 (0.940)3 (0.050) 3/264 NT 5.2% coding (700/969 nt) astE succinylglutamate desuccinylase
?NC_000913 = 1826327 56 (0.990)coding (597/969 nt) astE succinylglutamate desuccinylase
* ? NC_000913 = 182887259 (1.000)4 (0.070) 4/258 NT 6.4% coding (863/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
?NC_000913 = 1828893 61 (1.100)coding (842/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
* ? NC_000913 = 220995855 (0.930)3 (0.050) 3/260 NT 5.7% pseudogene (859/2001 nt) yehQ pseudogene
?NC_000913 = 2209979 48 (0.860)pseudogene (880/2001 nt) yehQ pseudogene
* ? NC_000913 = 238526461 (1.030)3 (0.050) 3/266 NT 5.1% coding (459/1728 nt) yfbK Von Willebrand factor domain putative lipoprotein
?NC_000913 = 2385282 54 (0.950)coding (441/1728 nt) yfbK Von Willebrand factor domain putative lipoprotein
* ? NC_000913 = 244659553 (0.890)5 (0.090) 3/268 NT 9.5% coding (879/1086 nt) aroC chorismate synthase
?NC_000913 = 2446636 44 (0.760)coding (838/1086 nt) aroC chorismate synthase
* ? NC_000913 = 245506074 (1.250)6 (0.110) 3/258 NT 7.7% intergenic (‑59/+23) yfcU/yfcV pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein/putative fimbrial‑like adhesin protein
?NC_000913 = 2455066 75 (1.350)intergenic (‑65/+17) yfcU/yfcV pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein/putative fimbrial‑like adhesin protein
* ? NC_000913 = 272945232 (0.540)3 (0.050) 3/256 NT 8.4% intergenic (‑8/+164) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 4168342 = 36 (0.660)intergenic (+142/‑30) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 2729475 =38 (0.640)3 (0.050) 3/256 NT 8.8% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168363 27 (0.490)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 = 290053086 (1.450)5 (0.090) 4/254 NT 5.6% intergenic (‑257/‑62) ygcW/yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
?NC_000913 = 2900548 88 (1.610)intergenic (‑275/‑44) ygcW/yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
* ? NC_000913 3153895 =54 (0.910)3 (0.050) 3/266 NT 5.7% coding (333/660 nt) yghB general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
?NC_000913 3153945 = 47 (0.820)coding (383/660 nt) yghB general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
* ? NC_000913 3198857 =44 (0.740)4 (0.070) 4/268 NT 9.1% coding (785/2841 nt) glnE fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
?NC_000913 3198892 = 37 (0.640)coding (750/2841 nt) glnE fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
* ? NC_000913 3583431 =NA (NA)4 (0.070)
+CCC
4/270 NT 100% noncoding (4/768 nt) IS1 repeat region
?NC_000913 4640551 = 0 (0.000)intergenic (+9/‑391) yjjY/yjtD uncharacterized protein/putative methyltransferase
* ? NC_000913 3605170 =79 (1.330)5 (0.090) 5/254 NT 6.6% coding (192/270 nt) yhhL DUF1145 family protein
?NC_000913 3605202 = 69 (1.270)coding (224/270 nt) yhhL DUF1145 family protein
* ? NC_000913 3726887 =91 (1.540)5 (0.090) 5/254 NT 5.6% intergenic (+5/+37) wecH/yiaA O‑acetyltransferase for enterobacterial common antigen (ECA)/YiaAB family inner membrane protein, tandem domains
?NC_000913 3726917 = 85 (1.560)intergenic (+35/+7) wecH/yiaA O‑acetyltransferase for enterobacterial common antigen (ECA)/YiaAB family inner membrane protein, tandem domains
* ? NC_000913 4326878 =99 (1.670)6 (0.110) 6/258 NT 6.2% intergenic (‑144/‑257) yjdM/crfC zinc‑ribbon family protein/clamp‑binding sister replication fork colocalization protein, dynamin‑related
?NC_000913 4326902 = 88 (1.590)intergenic (‑168/‑233) yjdM/crfC zinc‑ribbon family protein/clamp‑binding sister replication fork colocalization protein, dynamin‑related