Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F53 I0 R2 221 66.0 2680176 94.9% 2543487 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,274 C→T 100% E185K (GAA→AAA)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,2740CT100.0% 238.9 / NA 70E185K (GAA→AAA) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/39);  total (31/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGT  >  CP006881/3724146‑3724409
                                                                                                                                |                                                                                                                                       
aaGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTtctc                                                                                                                                <  5:3343/138‑1 (MQ=255)
      ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTTTTTTCTCTATCAtt                                                                                                                         >  7:156878/1‑139 (MQ=255)
      ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                         >  7:246312/1‑139 (MQ=255)
      ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                         >  5:234938/1‑139 (MQ=255)
         ctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                      <  1:183851/139‑1 (MQ=255)
           cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCttt                                                                                                                    >  3:126474/1‑139 (MQ=255)
            gTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCTTTTCTTTg                                                                                                                   >  5:178285/1‑139 (MQ=255)
             tCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGa                                                                                                                  >  7:91793/1‑139 (MQ=255)
               cATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATATGATATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGAt                                                                                                                 <  3:199682/138‑1 (MQ=255)
                aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                               <  6:234938/139‑1 (MQ=255)
                 tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                                                                                              >  4:199692/1‑139 (MQ=255)
                 tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTATCTATCATTTCTTTGATCAt                                                                                                              >  2:219592/1‑139 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTAATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                         <  5:176797/139‑1 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTTTCATTTCTTTGATCATGGTCg                                                                                                         >  8:156227/1‑139 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                                                                                            >  1:64864/1‑136 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                                                                                            <  2:64864/136‑1 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                         >  3:223516/1‑139 (MQ=255)
                      tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTACGATTAAAGTATTGTTTTATTTTCTCTATGATTTCTTTGATCATGGtag                                                                                                         >  2:289248/1‑137 (MQ=255)
                        gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                       <  2:45930/139‑1 (MQ=255)
                        gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                       <  6:292490/139‑1 (MQ=255)
                        gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                       <  4:223516/139‑1 (MQ=255)
                         aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTAt                                                                                                      >  4:197480/1‑139 (MQ=255)
                          gcgcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATg                                                                                                     >  1:65711/1‑139 (MQ=255)
                           cgcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATga                                                                                                    >  5:220592/1‑138 (MQ=255)
                             cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                                                                                  <  1:219592/139‑1 (MQ=255)
                             cTTCATTTGATTCCAACCGGCTGACCGCGTAGAGGAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCTTCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGTTCATGGTCGTATGCtt                                                                                                  <  3:131456/139‑1 (MQ=255)
                              ttCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                                                                                  >  7:3936/1‑138 (MQ=255)
                              ttCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTATTTGATCATGGTCGTATGCtt                                                                                                  >  1:1431/1‑138 (MQ=255)
                              ttCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTAGATAGCAAAAGCTGTTTTCATCCACCTTTCGATTAAAGAATTGTATTATTTTCTCTATCATTTCTTTAAACATCGAAGTATTCtt                                                                                                  >  4:280032/1‑138 (MQ=255)
                                cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                                <  7:116002/138‑1 (MQ=255)
                                cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                                <  8:247570/138‑1 (MQ=255)
                                  tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                             <  7:33437/139‑1 (MQ=255)
                                  tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                             <  2:312132/139‑1 (MQ=255)
                                  tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                             <  7:153511/139‑1 (MQ=255)
                                  tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                             <  2:177356/139‑1 (MQ=255)
                                    tGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcgc                                                                                            <  4:126474/138‑1 (MQ=255)
                                       ttCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa                                                                                        <  3:197480/139‑1 (MQ=255)
                                        tCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa                                                                                        >  1:308528/1‑138 (MQ=255)
                                        tCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTAAGATTCAGCGCTGAAt                                                                                       >  4:131456/1‑139 (MQ=255)
                                          cAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATa                                                                                      <  7:119642/138‑1 (MQ=255)
                                               ggctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGct                                                                                <  5:135130/137‑1 (MQ=255)
                                                     ccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATGATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATTCTCACCGc                                                                          >  6:223061/3‑139 (MQ=255)
                                                     ccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCg                                                                           >  2:187845/3‑138 (MQ=255)
                                                           aTCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccat                                                                    >  5:127984/1‑139 (MQ=255)
                                                              gcAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCtt                                                                 <  2:148266/138‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTTATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGGATCCATCTTCg                                                               <  3:280032/139‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                               <  2:1431/139‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                               <  8:3936/139‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                               <  2:65711/139‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                               <  4:118319/139‑1 (MQ=255)
                                                                aaaTGCGTGACAAGGCGTTGATAGGAAAAGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                               <  4:302208/139‑1 (MQ=255)
                                                                  aTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGcc                                                             >  8:17703/1‑139 (MQ=255)
                                                                     cGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTa                                                          <  7:7988/139‑1 (MQ=255)
                                                                       tGACAAGGCGTTGATATGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTATATCATTTCTGTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTGAGCCGTATg                                                        >  7:24392/1‑139 (MQ=255)
                                                                              gCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                                <  1:99998/92‑1 (MQ=255)
                                                                              gCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                                >  2:99998/1‑92 (MQ=255)
                                                                               cGTTGATAGGTAATGCTGTTTTCATCGACCTTTAGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGTTGTGaa                                                <  6:127984/139‑1 (MQ=255)
                                                                               cGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCATCGCCGTATGGAtgtg                                                  >  7:137147/1‑137 (MQ=255)
                                                                                       ggACATGCTGTTTTCATCAACCTTTAGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCCTGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAat                                         <  8:24392/138‑1 (MQ=255)
                                                                                                 ttttCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCtt                              >  7:122275/1‑139 (MQ=255)
                                                                                                   ttCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCa                            >  7:65263/1‑139 (MQ=255)
                                                                                                       tCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCtt                        <  2:308528/139‑1 (MQ=255)
                                                                                                           ccTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccatc                                                                   >  3:218558/1‑92 (MQ=255)
                                                                                                           ccTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatccatc                                                                   <  4:218558/92‑1 (MQ=255)
                                                                                                                   tttaaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGaca            <  7:141105/136‑1 (MQ=255)
                                                                                                                   ttAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGaca            <  5:231738/139‑1 (MQ=255)
                                                                                                                      aaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACg          <  3:199692/138‑1 (MQ=255)
                                                                                                                      aaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTATCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCCACTTCATGCAGAGACACGc         >  6:105754/1‑139 (MQ=255)
                                                                                                                             gTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATg   <  8:91793/138‑1 (MQ=255)
                                                                                                                             gTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt  <  8:246312/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
AAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGT  >  CP006881/3724146‑3724409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A