Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F54 I0 R2
|
227 |
88.8 |
3424874 |
95.5% |
3270754 |
127.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
2,991,169 |
A→T |
53.9% |
intergenic (‑5/+13) |
U712_21340 ← / ← U712_21345 |
tRNA‑Leu/tRNA‑Gly |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,991,169 | 0 | A | T | 53.9%
| 1.3
/ 7.7
| 20 | intergenic (‑5/+13) | U712_21340/U712_21345 | tRNA‑Leu/tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base A (3/6); new base T (6/3); total (11/9) |
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGT > CP006881/2991031‑2991294
|
tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCAGCCCGGCGTGGa < 8:113746/139‑1 (MQ=17)
tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa < 1:396226/139‑1 (MQ=32)
tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa < 1:425143/139‑1 (MQ=32)
tCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa < 6:328998/139‑1 (MQ=32)
ccGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGa < 6:73050/138‑1 (MQ=32)
gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCgtgggtgg > 1:364851/1‑139 (MQ=17)
gTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATgg > 5:293955/1‑139 (MQ=32)
ctctATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGGTGGTTTATTTgggg > 8:162684/1‑137 (MQ=255)
tATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGGCTAGTTGGGGCg > 7:113896/1‑138 (MQ=12)
cAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGa < 7:174661/139‑1 (MQ=32)
cAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGa < 6:229458/139‑1 (MQ=32)
aTTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACATACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCTGCTTGGTTGGTTTAGTTGGAGCGGCAGACg > 8:12500/1‑139 (MQ=11)
ttGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTCCCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTGGTTTTGTTGGAGCGGAAGACgg > 8:118368/1‑139 (MQ=255)
tGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTTGTATTTTTTGAGCGGAAGACggg > 6:409756/1‑139 (MQ=11)
gAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCCTGGTTTCTATTGTTTGAACGGAAAACg > 4:366281/1‑136 (MQ=11)
aGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTAGGGTTAGTTGGAGGGGATGCCgggtt > 6:366894/1‑137 (MQ=11)
aGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGAt > 7:277313/1‑139 (MQ=32)
cTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGGTGGTTTAGTTGGAGCGGAAGACGGGGttg > 1:10428/1‑138 (MQ=12)
cgcAAAACTTAATCCCGATGGCCGGACTTCAACCGGCACGGTAGTCACCTACCGCCGGATTTAAAGTCCGGGGTGTCTGCCACTGCCACCACCCCGGCTGGGTTTGTATAGTTGGTGGGGAAGCCGGGATTCGAAc < 4:129001/136‑1 (MQ=11)
gcAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGTTGGGATTTTTGGATGGGAAGACGGGGTTCCAACCcgc > 7:323941/1‑139 (MQ=11)
gcgggACTTGACCCGTCAGGGTAGTACCCTACCGCTGTTTTTTAAGTCGTGTGTGTGTGCCATTCCCACCCCCCGGGGGTGTTTGTTATAGTTGGTGGGTACCAGGGGATTCCAACCCGCGACCCCCACCTTGGCAAgg < 8:99668/136‑1 (MQ=0)
gATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGCGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGt < 7:276160/139‑1 (MQ=21)
cgtgtgtgTCTGCCAATACCACCACCCGGTCGTGTTTGTTAAAGTGGCAGCGGAAGACGGGATACCACCCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAg < 5:366894/137‑1 (MQ=14)
tgtcgcccATTTCCCCCACCCGGGCTTGTATTGTATAGTTGGCGGGTAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACtt < 7:404944/133‑1 (MQ=17)
atccccccaccCCGGCGTGGATGTTATAGTTGGCGCGGAAGCCGGGATTCCAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCa < 8:395293/133‑1 (MQ=17)
caccCGGGCGTGGATGGTATAGTTGGCGCGGAAGACGGTATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGt < 8:323941/139‑1 (MQ=255)
|
TCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGCAAAACTTAATGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGCGTGGATGGTATAGTTGGAGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGCAGAAATGGTGCGGATGAAGGGACTTGAACCCCCACGTCTGT > CP006881/2991031‑2991294
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
