Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F21 I0 R1 50 53.6 2034412 90.9% 1849280 142.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 699,559 A→G 8.2% intergenic (‑382/+15) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,5590AG 8.2% 129.3 / 3.2 53intergenic (‑382/+15)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base G (3/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

TTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCG  >  NC_000913/699417‑699700
                                                                                                                                              |                                                                                                                                             
ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCa                                                                                                                                               <  2:1009534/143‑1 (MQ=255)
 tGTTCATGCCGGATGAGGCGTTAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAg                                                                                                                                              <  1:781552/143‑1 (MQ=255)
    tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCCCGTCCGCAGACg                                                                                                                                           >  1:918458/1‑143 (MQ=255)
      aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTCCAGGAGCTGCGTAGGCCTGATAAGCCCAGCGCATCAGGCAGTTTGGCGTTTGTCATCAAAGCCAACCACGTCCGCAGCCggg                                                                                                                                         >  2:45544/1‑141 (MQ=21)
         ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGtt                                                                                                                                      <  2:684652/143‑1 (MQ=255)
           ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGc                                                                                                                                    <  2:837129/143‑1 (MQ=255)
                 ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCACTATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGTGCCTACCACGTCCGCAGACGTGGTTGCTATTCa                                                                                                                              <  2:122501/143‑1 (MQ=255)
                  gcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAATCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTGCGCAGCCGTGGCTGCTCTTccg                                                                                                                             >  1:429416/1‑141 (MQ=21)
                      gAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCACTCTAATGCATGAGCTGCGAAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGATCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa                                                                                                                         <  1:904597/143‑1 (MQ=25)
                       aaCGCCTTATCCGGCCTACAAAATCATGAAAATAACTTAATTTTCAGGAGCTGCGTAGGCTTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAAc                                                                                                                        <  2:446655/143‑1 (MQ=25)
                           ccTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCg                                                                                                                    >  2:271787/1‑143 (MQ=255)
                                   ggCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                                            <  1:490153/143‑1 (MQ=255)
                                     ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGa                                                                                                          <  2:12571/143‑1 (MQ=255)
                                       tACAAAAGCAGGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGCTAACGTCGATTTCAGCGACt                                                                                                        >  2:221551/1‑143 (MQ=255)
                                           aaaGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCCGGCAGTTTGGCGTTTGTCATCAGACCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACg                                                                                                    <  2:502519/143‑1 (MQ=255)
                                                   aaaaTTCAATAGATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTACGATAACGGCTATTTCAGCGACTGACGGGTAAATc                                                                                            <  2:888578/143‑1 (MQ=37)
                                                            tatatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                                                   >  1:167753/1‑143 (MQ=255)
                                                              gatggCAGGAGGTGCGGAGGCCGGATAAGCGTAGCGCATGAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGGCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                                                 <  2:514298/139‑1 (MQ=255)
                                                                ttGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGa                                                                               >  1:919843/1‑143 (MQ=255)
                                                                  gCACGAGCTGCGTAGGCCTGATAAGCGTAGCGCACCAGGCAGTTTCGCGCTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa                                                                             <  1:846658/143‑1 (MQ=255)
                                                                   cttgAGCTGCGTAGGCCTGATAAGCGGAGCGCATCAGGGAGGTTGGCGTTTGTCATCAGAGCCAACCACGTTCGCAGACGTGGTTGCTATACAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAg                                                                            <  2:866875/140‑1 (MQ=255)
                                                                            cGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATCTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGt                                                                   >  2:993708/1‑143 (MQ=255)
                                                                              tAGGCCTGATAGGCGTAGCGCATCAGGCAGTTTGGTGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCg                                                                 <  2:90751/143‑1 (MQ=255)
                                                                              tAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCg                                                                 >  1:589998/1‑143 (MQ=255)
                                                                                gggcTGATAGGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGat                                                               <  2:961537/140‑1 (MQ=255)
                                                                                        atgCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTTGTTGCTATTCAGATAACGTCGATTTTAGCGACTGTCGTGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                                       <  2:70505/141‑1 (MQ=255)
                                                                                        aaGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                                       >  2:527473/1‑143 (MQ=255)
                                                                                        aaGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                                                       <  1:768918/143‑1 (MQ=255)
                                                                                         agagTAGCGCATCAGGCAGTTTGGCGTTTGTTATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTTGATTTCAGCGACTGACGGTTAAATCCAGCTGGGGCTGAAAGGCATACTGTCGATATCGTCGAg                                                      <  2:771648/140‑1 (MQ=255)
                                                                                               gcgcATCATGCAGTTTGGCGTTTGTCATCAGAGCCACCCACGTCCGCAGACGTGGTTGCTATTCAGATCACGTCGATTTAAGCGACTGACGGGTAAATCCAGCTGAGGCGGAAGGGCATACTGTCGCTATAGCCGCGcgacga                                                >  2:998891/1‑143 (MQ=255)
                                                                                               gcgcATCAGGCAGTTTGGCGTTTGTCATCAGCGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCCGCGACTGACGGGTAAGTCGAGCTGGGGCGGAAAAGCATACTGTAGATAGCGTAGAGcggcga                                                >  2:829642/1‑143 (MQ=255)
                                                                                                cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcgcagca                                               >  2:596420/1‑138 (MQ=255)
                                                                                                 gcATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                              <  2:731026/143‑1 (MQ=255)
                                                                                                 gcATCAGGCAGTCTGGCGTTTGTCATCAGAGCCACCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                              <  1:80860/143‑1 (MQ=255)
                                                                                                 ccaggcggCAGTTGGGCGTGTGTCGTCAGCGCCAACCAGGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAGCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                                              <  1:80859/137‑1 (MQ=255)
                                                                                                       ggCAGTTCGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAg                                        <  1:884735/143‑1 (MQ=255)
                                                                                                          aGTCTGGCGTTTGTCATAAAATCCATCTGCGTCCGCAGACGTGGTTGCGATGCAGATAACGTCCATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaaa                                     <  2:657813/143‑1 (MQ=255)
                                                                                                           gTTTGGCGTTTGTCATCAGAGGCGAGCAGGTCCGCAGACGTGGTTGCGATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAg                                    <  1:271787/143‑1 (MQ=255)
                                                                                                            tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGCTTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATAGTGTGGATATCATCGAGCGACGAAACACCAGAAAga                                   >  1:686252/1‑142 (MQ=255)
                                                                                                               ggCTTTTGTTAGCCGAGCCAACCACGTCTGCGGACGTTGTTGTTCTTCTGTTTATGTAGATCTCTGTGTCTGACGGGTAAATCCAGCTGGGGCGGAACGGCATCCTGTCGTTATCGTCGAGCGACGAAACACCAGAAAGCAcc                                <  2:258964/143‑1 (MQ=255)
                                                                                                                  gTTTGTCATCAGAGCCAACCACGTCAGCAGACGTGGGTGCTATTCAGATAAAGTCGACTTCAGCGACTGACGGGTAAATCCAGCTGGAGCGGAAAGGCATACTGTCGGTATCGACGAGCGACGAAACACCAGAAAGCGCCaca                             >  2:646084/1‑141 (MQ=255)
                                                                                                                      gTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAACGCACCAGAATCg                         >  2:675239/1‑143 (MQ=255)
                                                                                                                       tcatcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGCCGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGGGCCACGAAACACCAGAAAGCACCAGACTcca                        >  2:863167/1‑141 (MQ=255)
                                                                                                                        catcaGAGCCAGCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCGCCAGAATCGtc                       <  2:256779/143‑1 (MQ=255)
                                                                                                                            agtgCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa                   <  2:181984/140‑1 (MQ=255)
                                                                                                                            agagCCAGCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa                   <  2:918458/143‑1 (MQ=255)
                                                                                                                            agagCCAACCACGTCCGCAGACGTGGATGCTATTCAGTGGACTTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa                   <  1:184891/143‑1 (MQ=255)
                                                                                                                                ccAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAACGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGTCACCAACAGAACCGTCGCCAGAca               >  1:168797/1‑142 (MQ=255)
                                                                                                                                ccAACCACGTCCGCAGACGTGGTTGCTAGTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGACCGGCATACTGTCGATATCGTCGAGCGACGACACACAAGAACGCACCAGCATCGTCTCCAGccc               >  2:876783/1‑143 (MQ=255)
                                                                                                                                  aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAACGCACCAGAATCGTCTCCAGAcctg             >  1:251778/1‑143 (MQ=255)
                                                                                                                                  aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctg             >  1:905687/1‑143 (MQ=255)
                                                                                                                                  aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGACTCCAGCGACTGACGGGTATATCCATCGGGGGCGCCAAGGAATCCCGCCGATATCGACGAGCGACGACCCACCAGCACGCACCACAATAGTccccccaaccc             >  2:494240/1‑136 (MQ=255)
                                                                                                                                  aaCCACGTCCGCAGACGTGGATGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGCCGGAAAGGCATACTGTCGATATCGTCGCGCGACGACACACCCGAAAGCACCACAAACGTCGCGAGAccgg             >  2:590859/1‑141 (MQ=255)
                                                                                                                                             cAGACGTGGTTGCTATTCAGATAACGTCGATCTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCg  <  1:993708/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAGCCG  >  NC_000913/699417‑699700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A