Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F61 I0 R1
|
36 |
35.4 |
1352518 |
90.1% |
1218618 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
710,436 |
G→A |
100% |
H71Y (CAC→TAC) |
fur ← |
DNA‑binding transcriptional dual regulator Fur |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 710,436 | 0 | G | A | 100.0%
| 90.9
/ ‑6.7
| 33 | H71Y (CAC→TAC) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/17); minor base C (1/0); total (16/17) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGA > NC_000913/710296‑710572
|
ttttGCGGCAATTTCTCGCTGACGCGCTTCGATGGAATCATCACTAAATGCGATAACCTTGCCGCAGTAGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGc < 2:119325/143‑1 (MQ=255)
ggCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAACTGTCGCGGGTGa > 1:645873/1‑143 (MQ=255)
gCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATCCGATACCCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGAc < 1:327089/143‑1 (MQ=255)
tttCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCCGTCGAGGCAGATCACGTGATCGTGGTGATGTTGCTGTGTCAGTTCACATACGGATTTCCCCCCTTCAAAATTGTAGCCGGTGACGATa > 1:249969/1‑143 (MQ=255)
tCACGCTGACGCGCTTCGATGGGATCATCACTAAATGCGATGACCTTGCGGCAGTCGGGGCAGATCAGGTGATCGTGGTGATGTGGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGGGACGATAcc > 1:496929/1‑143 (MQ=255)
aCGCTGACGCGCTTCGATGGAATCATCACTAAATTCGCTAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAg < 2:44506/143‑1 (MQ=255)
aCGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCGGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTAAGTTCAACTAGGGATTTACCGTCGTCAAAAGTGTAGCGGGTGGCGATACCAg > 2:606527/1‑143 (MQ=255)
gcgcTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCa > 1:198172/1‑143 (MQ=255)
ttCGCTGTAATCATCACTAAATTCGATACCCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACt < 1:145588/143‑1 (MQ=255)
cGATGGAATCATCACTAAATTCGATAACCTTGGCGCAGTCGAGGCAGATCTGGCCCTTCGCGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑gg < 1:490210/143‑1 (MQ=255)
cGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑gg < 1:574322/143‑1 (MQ=255)
aTGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGtt > 1:459937/1‑143 (MQ=255)
ggATTCATCATTAAATTCGATAACCTTGCCGTAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTCCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCa < 2:338101/143‑1 (MQ=255)
ggAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCa < 1:256763/143‑1 (MQ=255)
aTAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGAAGATACCAGCGTCGTCAAACT‑GGTTCACGACGCGATATACCGTAGc > 2:247102/1‑143 (MQ=255)
agctttGCCGCAGTCGCGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCa < 2:604845/139‑1 (MQ=255)
cTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGAc > 1:215614/1‑143 (MQ=255)
cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATAACAGCGTCGTAAAACT‑GGTTCAGTACGCGCTATACCGTAGCCAGACCaaaa > 2:88714/1‑141 (MQ=255)
gATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAACATTGTAGCGGGTGACGATACCAGCGTCGTCAAACA‑GGTTCAGTACGCGATATACCGTAGCCCGACCGATTTCTTCACCCatat > 2:127051/1‑143 (MQ=255)
gTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGCTCa > 1:574500/1‑143 (MQ=255)
cggggtgATGTTGTTGTGTCAGTTCACATACGGGTTTACCGCCTTCAAACTTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGt < 2:361709/140‑1 (MQ=255)
gATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGtat > 1:325881/1‑143 (MQ=255)
gCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑AGTTCAGTACGCGATATACCGGAGGCAGACCAATTTCTTCACCGATAGCGCGCAGACGCTAGCATaaagat > 2:63951/1‑140 (MQ=255)
gtgtAAGTTCAAATAGGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATAGATCAGACGTTTGTATAAATCTTcc < 1:156100/143‑1 (MQ=255)
aaaGACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGt < 1:106277/143‑1 (MQ=255)
aTACGGATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGa < 2:198172/143‑1 (MQ=255)
cGGATTTAGCGCCTTCAATATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATgg < 2:392868/143‑1 (MQ=255)
ggATTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGt > 2:496196/1‑143 (MQ=255)
taTTTACCGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGtt < 2:215614/142‑1 (MQ=255)
cGCCTTCAAAATTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGc < 2:459937/143‑1 (MQ=255)
gCCTTCCAAATGGTAGCGGGTGACGATACCCGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCt < 1:198815/143‑1 (MQ=255)
aaaaTTGTAGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgac > 1:469905/1‑142 (MQ=255)
aaTTGTAGCGGGTGACGATACCCGCCT‑ATCAAAATACGTACAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGAGGGTTGTCCGGCTCCTgagga > 2:205894/1‑143 (MQ=255)
|
TTTTGCGGCAATTTCACGCTGACGCGCTTCGATGGAATCATCACTAAATTCGATAACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACT‑GGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGA > NC_000913/710296‑710572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
