breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 119,114 Δ1 bp 12.1% coding (382/552 nt) ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
RA 119,115 Δ1 bp 12.1% coding (383/552 nt) ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
RA 125,387 C→T 100% Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 397,574 C→T 18.9% L312L (CTC→CTT sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 710,436 G→A 100% H71Y (CAC→TAC)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 759,250 G→T 100% R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,391,775 IS1 (+) +9 bp 100% coding (1259‑1267/1530 nt) nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,425,968 T→G 12.1% intergenic (‑52/+38) hisQ ← / ← hisJ lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ/histidine ABC transporter periplasmic binding protein
RA 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,740,698 C→T 10.3% P151P (CCG→CCA aroF ← 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive
RA 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,080,006 T→C 11.8% K544E (AAA→GAA)  tktA ← transketolase 1
RA 3,168,653 G→A 8.5% intergenic (‑109/+96) mqsR ← / ← ygiV mRNA interferase/toxin of the MqsR‑MqsA toxin‑antitoxin system/DNA‑binding transcriptional repressor YgiV
RA 3,288,032 C→A 8.5% intergenic (+22/‑58) yraH → / → yraI putative fimbrial protein YraH/putative fimbrial chaperone YraI
RA 3,288,033 T→G 8.6% intergenic (+23/‑57) yraH → / → yraI putative fimbrial protein YraH/putative fimbrial chaperone YraI
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,971,996 T→A 12.7% L246Q (CTG→CAG)  wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,094,415 T→G 11.8% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,196 T→C 32.6% intergenic (‑97/+136) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,196,249 C→A 17.7% intergenic (‑150/+83) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,273,362 T→A 24.4% A170A (GCA→GCT uvrA ← excision nuclease subunit A
RA 4,296,060 C→T 38.5% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,341,824 T→G 33.8% intergenic (‑196/‑87) melR ← / → melA DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4168393–4168478 4171534–4168511 34–3142 9 [7] [8] 10 [gltT]–[rrlB] [gltT],[rrlB]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 304784 =34 (1.000)3 (0.090) 3/252 NT 8.6% coding (422/711 nt) ecpE putative fimbrial chaperone EcpE
?NC_000913 304813 = 31 (0.950)coding (393/711 nt) ecpE putative fimbrial chaperone EcpE
* ? NC_000913 315563 =NA (NA)3 (0.100) 3/244 NT NA noncoding (335/1255 nt) IS3 repeat region
?NC_000913 315599 = NA (NA)noncoding (371/1255 nt) IS3 repeat region
* ? NC_000913 = 315852NA (NA)19 (0.580) 8/256 NT NA noncoding (624/1255 nt) IS3 repeat region
?NC_000913 = 315875 NA (NA)noncoding (647/1255 nt) IS3 repeat region
* ? NC_000913 = 315916NA (NA)7 (0.210) 5/256 NT NA noncoding (688/1255 nt) IS3 repeat region
?NC_000913 = 315925 NA (NA)noncoding (697/1255 nt) IS3 repeat region
* ? NC_000913 392648 =NA (NA)4 (0.120) 3/250 NT NA noncoding (319/1258 nt) IS3 repeat region
?NC_000913 392679 = NA (NA)noncoding (288/1258 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1534 (45.410) 248/262 NT 98.9% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 17 (0.500)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =3 (0.090)16 (0.540) 15/232 NT 80.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 5 (0.170)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078053 (0.090)13 (0.430) 12/232 NT 77.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 5 (0.170)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2729475 =24 (0.700)3 (0.100) 3/244 NT 14.9% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA
?NC_000913 = 4168363 12 (0.380)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA/tRNA‑Glu
* ? NC_000913 2729955 =NA (NA)3 (0.090) 3/252 NT NA noncoding (1203/1542 nt) rrsG 16S ribosomal RNA
?NC_000913 2729972 = NA (NA)noncoding (1186/1542 nt) rrsG 16S ribosomal RNA
* ? NC_000913 = 384799826 (0.760)3 (0.090) 3/256 NT 10.5% coding (627/1320 nt) uhpC inner membrane protein sensing glucose‑6‑phosphate
?NC_000913 = 3848018 26 (0.790)coding (607/1320 nt) uhpC inner membrane protein sensing glucose‑6‑phosphate
* ? NC_000913 4542682 =37 (1.090)4 (0.130) 4/246 NT 11.7% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 26 (0.820)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit