Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F44 I0 R1
|
30 |
22.2 |
989614 |
86.8% |
858984 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
710,489 |
G→C |
100% |
A53G (GCT→GGT) |
fur ← |
DNA‑binding transcriptional dual regulator Fur |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 710,489 | 0 | G | C | 100.0%
| 60.3
/ ‑6.7
| 25 | A53G (GCT→GGT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (14/8); minor base A (1/0); total (15/10) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAG > NC_000913/710350‑710615
|
aaCCTTGCCTCTCTCGAGTCAGACGCGGTGATCGCGGTGATGTTTGTGTGCCAGTTCAAATACGGATTTACCGCCTTCAAAACTGTGGCGGGTGACGATACCAGCTTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCa < 2:125561/143‑1 (MQ=255)
aGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGTTTTACCTCCTTCAAAATTGTAGCTGGTGACGATACCAGCGTCGTTAAACTTGTTCAGTACGCGATATACCATAACCAGACCAATTTCTTCAcc > 1:133629/1‑143 (MQ=255)
aGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATATGTATTTACCGTCTTCAAAATTGTGGCTTGTGACGATACCAGCGTCGTCAAACTGGTGCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 1:136080/143‑1 (MQ=255)
gATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTTGTCAAACTGGTTAAGTACGCGATATACCGTACCCAGACCAATTTCTTCACGCATATCGAttgga > 1:295615/1‑139 (MQ=255)
aTCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCAGACCCAGACCAATTTCTTCAACCACATGGACAAGAc > 2:323471/1‑143 (MQ=255)
gtgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGt > 1:356740/1‑143 (MQ=255)
gtgtCAGTTCAAATACGGATTTACCACCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAACAAACATTTGTATAAATATTcc > 2:98452/1‑143 (MQ=255)
tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCg < 1:252410/143‑1 (MQ=255)
tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCg > 1:441994/1‑143 (MQ=255)
gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTATCCACCCAAATCGATCAGACGTTTGTATAAATCTTACGCACTg > 2:122675/1‑143 (MQ=255)
tCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGAGCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGAc < 1:160540/143‑1 (MQ=255)
cGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGGACCCAGACCAATTTCTTAACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACgagagga > 2:449481/1‑140 (MQ=255)
ttACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTc < 2:356740/143‑1 (MQ=255)
tgGCGGGTGAGGATACCGGCGTCGTCAAACTGCTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTc < 2:489972/143‑1 (MQ=255)
tgGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATAGCGTACGCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAAGCTTCCGCACTGACGGGATGGTTGTACGGCGCTTGAAGAACtgc > 2:442541/1‑141 (MQ=255)
tgatgaTACCAGCGTCGTCAAACTGGTTCATTACGTTTTATACCGTACCCAGAGCATTTTCTTCAACCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGAtt < 1:227859/143‑1 (MQ=255)
gATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTa > 1:15353/1‑143 (MQ=255)
tACCAGCGTCGTCAAACTGGTTTAGTACGCGATATATCGTATTTATTTCAATTTTTTCACTCATATCGATGAGAAGTGTGTATAAATCATCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTaaa < 1:114685/143‑1 (MQ=255)
aaaCTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTa > 2:458224/1‑143 (MQ=255)
aCTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTCACTTGCTGGCACCCCTGCTCCTAAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACt > 1:324804/1‑143 (MQ=255)
tGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCTGACATTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCGCCGGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACttt < 1:383860/143‑1 (MQ=255)
ttCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAg < 2:292115/143‑1 (MQ=255)
aGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATATTCAGAACTGACATGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGcc > 1:106115/1‑143 (MQ=255)
gTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCCCTGCCGTCATGGTTGTCCGGCTCCTAAAAAACATCCAGGATATTTAAACGAGGAAGCGTAACTTTCAGGCCa > 1:423489/1‑143 (MQ=255)
tACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGCCGTTTGTATAAAACTTCCGCACTAACGTGAAGGAAATCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCTAg > 1:15542/1‑143 (MQ=255)
|
AACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAG > NC_000913/710350‑710615
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
