breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,085 Δ1 bp 100% coding (2069/2664 nt) aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
JC JC 568,268 IS1 (–) +9 bp 100% intergenic (+21/‑39) renD → / → emrE DLP12 prophage; protein RenD/multidrug/betaine/choline efflux transporter EmrE
RA 710,489 G→C 100% A53G (GCT→GGT)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 1,026,927 A→T 100% V30E (GTG→GAG)  mgsA ← methylglyoxal synthase
RA 1,196,220 C→T 100% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 100% T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C 100% L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G 100% L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,913,579 G→A 18.2% A413V (GCG→GTG)  prc ← tail‑specific protease
RA 1,940,584 C→A 100% R351L (CGT→CTT)  mepM ← peptidoglycan DD‑endopeptidase MepM
RA 1,941,326 C→T 100% D104N (GAT→AAT)  mepM ← peptidoglycan DD‑endopeptidase MepM
RA 1,956,975 G→T 100% H11Q (CAC→CAA torZ ← trimethylamine N‑oxide reductase 2
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,270,605 G→A 15.1% intergenic (+60/‑121) mepS → / → pdeN peptidoglycan DD‑endopeptidase/peptidoglycan LD‑carboxypeptidase/putative c‑di‑GMP phosphodiesterase PdeN
RA 2,416,239 C→T 100% T498M (ACG→ATG)  pta → phosphate acetyltransferase
RA 3,041,398 T→G 100% L29R (CTT→CGT)  ygfZ → folate‑binding protein
RA 3,486,157 A→C 100% E13A (GAA→GCA)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 23.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,366,854 T→G 9.7% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366214 366434 221 6 [4] [4] 5 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913 1196277 1211449 15173 6 [4] [4] 5 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423759–3424525 3424525 1–767 5 [4] [4] 5 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3621201–3622296 3653115–3652031 29736–31915 5 [4] [4] 5 [rhsB]–[insH‑11] [rhsB],yhhH,yhhI,yhhJ,rbbA,yhiI,yhiJ,yhiL,yhiM,yhiN,pitA,uspB,uspA,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,[insH‑11]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 381669NA (NA)3 (0.130) 3/250 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 1048415 =21 (0.860)3 (0.130) 3/252 NT 13.0% coding (277/747 nt) gfcC capsule biosynthesis GfcC family protein
?NC_000913 1048443 = 20 (0.860)coding (249/747 nt) gfcC capsule biosynthesis GfcC family protein
* ? NC_000913 = 235434613 (0.530)3 (0.130) 3/252 NT 17.4% coding (82/1260 nt) glpB anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
?NC_000913 = 2354369 16 (0.690)coding (105/1260 nt) glpB anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
* ? NC_000913 = 3944978NA (NA)4 (0.170) 3/252 NT 16.6% noncoding (1275/2904 nt) rrlC 23S ribosomal RNA
?NC_000913 = 4038811 21 (0.860)noncoding (1293/2905 nt) rrlA 23S ribosomal RNA
* ? NC_000913 4295980 =NA (NA)3 (0.130) 3/250 NT NA noncoding (32/37 nt) other REP321c
?NC_000913 4296062 = NA (NA)noncoding (1/37 nt) other REP321e