Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F76 I0 R1
|
30 |
23.0 |
980404 |
89.1% |
873539 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
710,489 |
G→C |
100% |
A53G (GCT→GGT) |
fur ← |
DNA‑binding transcriptional dual regulator Fur |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 710,489 | 0 | G | C | 100.0%
| 80.4
/ ‑6.7
| 30 | A53G (GCT→GGT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (11/18); minor base A (1/0); total (12/18) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAG > NC_000913/710358‑710625
|
cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATAACAGCGTCGTCAAACTGGTTCAGTACGAGATATACCGAACACACACCAAta > 2:418996/1‑142 (MQ=255)
aGTCGAGGCAGATCAGGTGATCGTGGTGCTGTTGCTGTGTCCGTTCACCTACGGCTTTACCGCCTTCAAAATTGTGGCGGATGACGATACCAGCGTCGTAAAACTAGGTCAGTACGCGATATACCGTACCCAGACCAATttct > 1:122421/1‑143 (MQ=255)
aGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAACCCGCCCAATTTCTTCAcc > 2:399026/1‑143 (MQ=255)
aGGTGATCTGGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 2:43047/143‑1 (MQ=255)
aGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGTTTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 1:446352/143‑1 (MQ=255)
tgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACGGTACCCAGACCAATCTCTTCACCCATATCGATCAGACGTTTGtc > 1:42624/1‑142 (MQ=255)
tGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATaa < 1:295252/143‑1 (MQ=255)
tGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATaa < 1:342221/143‑1 (MQ=255)
ttGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAAt < 1:344903/143‑1 (MQ=255)
tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAGATCTTCCg > 1:123503/1‑143 (MQ=255)
tCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCa < 1:4267/143‑1 (MQ=255)
tCAGTTCAAATACGGATTTACCGCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCa < 2:482812/143‑1 (MQ=255)
gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTg < 2:487537/143‑1 (MQ=255)
tCAAACACGGATCTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGAc < 1:22388/143‑1 (MQ=255)
aaTACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCACATCGATCAGACGTCTGTATAAAACTTCCGCACtaccgag > 2:298975/1‑137 (MQ=255)
tACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTACTTCACCCATATCGATCAGGCGGTTGTATAAATCTTCCGCGCTGACGTGag > 1:54327/1‑142 (MQ=255)
aCGGATTTACCGCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg < 1:313952/143‑1 (MQ=255)
tcgtcTTCAAGCTTGTGTCTGGTGAGGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGATCAATTTCTTCACCCTTATCTATCTGACGTTTGTATAAATCTTCCTCACTGACGTGATGGTTGTCCgg < 2:192270/139‑1 (MQ=255)
cTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTGAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGCTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTcc > 1:313794/1‑143 (MQ=255)
aaaaTTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgaa > 1:192916/1‑143 (MQ=255)
aTTGTGGCGGGTGACGATACCAGCGGCGTCAAACTGGTTCAGTACGCGATATCCCGTTCCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgaagaa < 2:290970/143‑1 (MQ=255)
tgGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTc < 1:42892/143‑1 (MQ=255)
gCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTATTCACCCATATCGATCAGACGTTTGTATAAATCTTCAGCACTGACGTGATGGTTGTCCGGCTCATGAAGAACTTCCa > 2:149137/1‑143 (MQ=255)
cGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAttttt < 1:418996/143‑1 (MQ=255)
cgtcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGc < 1:295666/143‑1 (MQ=255)
tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGAGGTTTGTATAAATCTTCCGCACTCCCGTGATGTGTGTCCGGCGTATCAACGACAGCCAGGATTTGTAAACGAGGacccgt > 2:78061/1‑138 (MQ=255)
accTGGTTCAGTACTCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTAc < 2:269195/141‑1 (MQ=255)
aCGCGTTATACCGTACCCAGACCAATTTCTTCACCCATCTCGATCAGACGTTTGTATACATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGc < 2:355597/143‑1 (MQ=255)
gcgATATACCGTACCCAGGCCAATTTCTTCACCCATATCGATCAGACGTTTGTATACATCTTCCGCACTTACGTGATGGTTGGCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGctt < 2:365863/143‑1 (MQ=255)
aCCGTACGCAGACCAATTTCTTCACGCATATCGATCAGACGTTTGTATACATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGTAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCGGCTTTCTCTAg > 2:369171/1‑143 (MQ=255)
|
CGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAG > NC_000913/710358‑710625
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
