Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F20 I1 R1
|
20 |
47.4 |
1967890 |
90.0% |
1771101 |
142.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
756,792 |
G→T |
E296* (GAA→TAA) |
sdhA → |
succinate:quinone oxidoreductase, FAD binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 756,792 | 0 | G | T | 95.8%
| 130.5
/ NA
| 48 | E296* (GAA→TAA) | sdhA | succinate:quinone oxidoreductase, FAD binding protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (25/21); total (26/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAT > NC_000913/756652‑756929
|
cGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCCGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTaa < 1:696440/143‑1 (MQ=255)
gggCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGTCCGTGACGTGGTCGCGCGTTCCACTATGATCTaaa > 1:41984/1‑143 (MQ=255)
gggCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTaaa > 1:666090/1‑143 (MQ=255)
ggTCACCGAAGGGGGCCGCGGTGCAGGCGGTTATCTGCTGAACAGCCGTGTCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAg < 1:36619/143‑1 (MQ=255)
gTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAgg > 1:693618/1‑143 (MQ=255)
tCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGAGTTCCATCATGGTCTAAATCAGAGAATGt > 1:814832/1‑143 (MQ=255)
ccGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTcgc < 2:922144/143‑1 (MQ=255)
ggggggTTGGCGTGGTGGAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTAGGGGGCGTTGTGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTcgcg < 2:436650/139‑1 (MQ=255)
cGAAGGTTGACGTGGTGAAGGCGGTTATATGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACATGGCGGGCCGTGACGTGGTTGCGCGTTCCATCCTGATGTAAAGCCGAGAAggcccca > 2:6600/1‑138 (MQ=255)
ggTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCtg > 2:221233/1‑143 (MQ=255)
cGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATgg > 1:408130/1‑143 (MQ=255)
ggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCGATCATGATCTAAATCCGTGAAGGGCGCGGCTGTGATGGTcc > 1:406229/1‑143 (MQ=255)
aaGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgg > 1:918801/1‑143 (MQ=255)
gCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTACATCCGTGGAGGTCGCGGCCGCGAGGCTGCGTggggg > 1:912230/1‑143 (MQ=255)
ctccgcggAACAAACACTGCGATCGTTGTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCacac < 1:216188/136‑1 (MQ=255)
ctgAACAAACGTGGCGTACGTTTTATGGTGCGTTATGCGCCGATCGCCAAATACCTGGCGGGCCGTGTCGTGGTTGCGCGTTCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaa < 1:572576/143‑1 (MQ=255)
ctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGCTGGTCCGTGGGGGCCACAcgcgca > 2:758362/1‑141 (MQ=255)
gAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaac > 1:643157/1‑143 (MQ=255)
acaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTACATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGCTGGTCCGTTGGGGCCACACGCgaaaccgcaac > 2:866308/1‑143 (MQ=255)
tGGCGAACGTGTTATGGAGCGTGGTGCTCCGAACGCCAATGAGCCTGCTTGGTGTGTCGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCg < 1:132261/143‑1 (MQ=255)
gttgtgtGGAGCGTGATGCGCCGAACGCCAACGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTg < 1:19986/137‑1 (MQ=255)
tttATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTggg > 2:710866/1‑143 (MQ=255)
gaggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGCTAaag < 2:813616/140‑1 (MQ=255)
aGCGTTATGCGCCGAACGCCAAAGACGTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa < 1:981255/143‑1 (MQ=255)
aGCGTTAGGGGCCGCACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGAGCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa < 2:849639/143‑1 (MQ=255)
tATGCGCCTAACGCCAAACTCCTTCCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAATGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtct < 2:79526/143‑1 (MQ=255)
aTGCGCGGATCGCCAAAGACCTGGCGGGTCGTGACGTGGTTGCGCGTTCCATCTTGATCTAAATCCGTGAAGGTCGCGGCTGAAATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc < 1:366501/143‑1 (MQ=255)
gcgcCGAACGCCAAAGACCTGGCGGGCCGTGCCGTGGTTGCTCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGa < 1:405765/143‑1 (MQ=255)
cgcCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGCAACTGAAACTCGATCACCTGGGTAAAGCAGTtctccca > 2:439034/1‑140 (MQ=255)
gcCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGCTCACCTGGGTCCAGAAGATGTCGAAt > 1:128121/1‑143 (MQ=255)
aaCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATAGCCTGGGGGAAGAAGGTCTCGAATCCCg > 2:382269/1‑143 (MQ=255)
ccACAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAAGCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTg < 1:761122/143‑1 (MQ=255)
cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACAAGCGAAACTGAAACTCGATCACCTGGGAAAACAAGCTGTCGAATCCCGTCTga > 2:287212/1‑142 (MQ=255)
aGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg < 1:710866/143‑1 (MQ=255)
aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGCAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCCCCTGGGTAAAGAAGTTCTCGAATCCCATCTGCCGGGt > 2:122583/1‑143 (MQ=255)
aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGACTCCCGTCTGCCGGGt > 2:185505/1‑143 (MQ=255)
aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGt < 1:803441/143‑1 (MQ=255)
ccTGGCGGGCCGTGACGTGGTTGCGCGTTTCATCATGATCTAAATTCTTGTGAAAGACGGCGGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTa < 1:221233/143‑1 (MQ=255)
cTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCCCACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAt > 1:491664/1‑143 (MQ=255)
tggCCGCGACGTGGTTGCGCGTTCCATCATGATCTACATTCGTGGAGGGCGCGGCTGTGCTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTgg < 1:764276/142‑1 (MQ=255)
ggCGGTGGCGGGGTGGCGGGGTCCAGCATGATCGAGATCCGGGAAGGTCGCGGCTGGGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa < 2:129961/143‑1 (MQ=255)
cGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGCAGCt > 1:280699/1‑143 (MQ=255)
gTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTGCTCGAATCCCGTCTGCCGGGTAGCCTGGAGCGTTCCCGTag > 2:460208/1‑142 (MQ=255)
tGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACCCGCGAACCTGAAACTCGATCACCTGGGTAAAGACGTTCTCGCATCCCGTCTGCCGGTGCTCCTGGAGCTTTCCCGTAccc > 1:174563/1‑142 (MQ=255)
gcgTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGATATACTGGAGCTTTACCGTACCTTCg > 1:633164/1‑143 (MQ=255)
ccATCATGAGCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCAc < 2:918801/143‑1 (MQ=255)
tcatGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGACGTTCTCGAATCCCGTCTGCCGGGTATCCTGGACCTGTCCCGCACCCTCGCtgtcgta > 2:93414/1‑137 (MQ=255)
tGATCTAAACCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt < 1:315407/143‑1 (MQ=255)
|
CGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAT > NC_000913/756652‑756929
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
