Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F52 I1 R1	23	44.3	1856968	90.0%	1671271	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,792	G→T	E296* (GAA→TAA)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,792	0	G	T	100.0%	75.7 / NA	27	E296* (GAA→TAA)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (12/15); total (12/15)

GGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCC  >  NC_000913/756654‑756931

ggCGTATTGGTTAGCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAAt                                                                                                                                         <  1:352566/143‑1 (MQ=255)

                      gCCGTGGTGAAGTCGGGTGTCTGCAGAACAAACATGGCGAACGTCGTATGGAGCGTGATGCGCCGAACGCCAAAGTCCGGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGAt                                                                                                                   <  2:518697/143‑1 (MQ=255)

                       ccGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGagg                                                                                                                  >  1:907316/1‑141 (MQ=255)

                          tggtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGCTCTAAATCCGTGAAGGTCGCGGCTGTGATGGTc                                                                                                               >  2:473952/1‑143 (MQ=255)

                          tggtgAAGGCGGTTATCTGCTGAACAAACACGGCGACCGCATTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCCTCATGCTCTAACTCCGTGAAGGTCGCGGCTGTGATGGTc                                                                                                               >  2:330247/1‑143 (MQ=255)

                              gagggcggcTATCTGCTGAGCAAACATGGCGAACGTTTTAGGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTg                                                                                                           <  1:150465/140‑1 (MQ=255)

                               aaGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTgg                                                                                                          <  1:386607/143‑1 (MQ=255)

                                            gctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCCCACGCga                                                                                             >  2:873078/1‑143 (MQ=255)

                                              tgAACGAACCGGGCGAACGTTTTATGGAGCGTTAGGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                                                                           <  1:419481/143‑1 (MQ=255)

                                              tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaa                                                                                           <  1:810397/143‑1 (MQ=255)

                                              tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTCGGGACCACACGCgaac                                                                                           >  2:175957/1‑142 (MQ=255)

                                                   aaacaCGGCGAACGATTAATTGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaa                                                                                      <  1:573253/143‑1 (MQ=255)

                                                                gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCACCGTGATCTAAATCCGTGGAGGTCCCGGCTCACAAGGTCCGTGGGGGGCTCACGCGAAACTGGAACTCGGTCGCCTg                                                                         >  1:721681/1‑143 (MQ=255)

                                                                         ggCGTCACGCGCCGACCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                                <  2:65853/142‑1 (MQ=255)

                                                                         aGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaagaa                                                                <  1:378898/143‑1 (MQ=255)

                                                                                 gcgcCCGACGCCAAATACCTGGCTGGCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGa                                                        <  2:496947/143‑1 (MQ=255)

                                                                                                         ggCCGTGAGGTGGGTGCGCGTGCCATCAGGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa                                <  2:272757/143‑1 (MQ=255)

                                                                                                          gCCGTGACGTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAg                               <  1:839275/143‑1 (MQ=255)

                                                                                                               gccGTGGTTTCGCGTTCCATCATGATCTAATTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGACGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTc                          <  1:266542/141‑1 (MQ=255)

                                                                                                                  gTGGTTGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTGCTCGAGTCCCGTCTGCCGGGTATCCGGCAGCGTTCCCg                       >  1:575195/1‑143 (MQ=255)

                                                                                                                    cgTTGCGCTTCCCGTCATGATCTGAACCCGTGAAGTTCGCGCCTTTGATGGTCCGTGTGGCGCACACGCGAAACTCAAACTCGATCACCTTGCTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTCGAGCTTTTCCTTa                     <  2:767970/142‑1 (MQ=255)

                                                                                                                       tGCGCGTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCAGGAGCTTTCCCGTAACt                  >  2:683382/1‑143 (MQ=255)

                                                                                                                          gcgtcgcatcttGATCTACATCCGTGAAGGGCGCGGCTGTGATGTTCCGTGGGGGCCAGACGCGAAACTGATACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg               <  2:762004/137‑1 (MQ=255)

                                                                                                                          gcgTTCCATCATGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGCAGTTCTCGAATCCCGTCTGCCGGGTATGCTGGAGCTTTCCCGTACCTTCg               >  2:664373/1‑143 (MQ=255)

                                                                                                                                catcatGATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACg         >  2:210624/1‑143 (MQ=255)

                                                                                                                                      gATCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGGGCTTTCCCGTACCTTCGCTCACGTCGATc   >  1:394996/1‑143 (MQ=255)

                                                                                                                                       aTCTAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGAGCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc  >  1:922287/1‑143 (MQ=255)

GGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCC  >  NC_000913/756654‑756931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A