Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F107 I0 R1
|
44 |
28.8 |
1140136 |
90.5% |
1031823 |
142.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,151,736 |
T→C |
81.7% |
V41A (GTT→GCT) |
acpP → |
acyl carrier protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,151,736 | 0 | T | C | 81.7%
| 35.1
/ 2.2
| 21 | V41A (GTT→GCT) | acpP | acyl carrier protein |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (6/11); total (8/13) |
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGA > NC_000913/1151612‑1151877
|
agTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTTAGCTGGCAATGGcgct > 2:443731/1‑143 (MQ=255)
aagaggCAGTAGCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTgg < 2:486849/138‑1 (MQ=255)
aTCGAATAGCGCGTTAAGAAAATTATCGGCGAACAGCTTGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGtt < 2:411830/143‑1 (MQ=255)
aCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTg < 1:336942/143‑1 (MQ=255)
aaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGAc < 1:497752/143‑1 (MQ=255)
tAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg < 2:412359/143‑1 (MQ=255)
gaagaaGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGc > 1:341516/1‑143 (MQ=255)
agaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTg > 1:334654/1‑143 (MQ=255)
aagTGACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCAGGCAGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGcc < 1:569978/143‑1 (MQ=255)
agTGACCAACAAGGCTTCGTTCGGTGAGGGCCTGGGCGCGGATGCTCTTGACACCGCTGAGCTGGTAATGGCGCTGGAAGAAGAGTTTGAGACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCa < 2:175471/143‑1 (MQ=255)
gTTACCAACAGTGCTTCTTTCGTTGAATTCCTGGGCGCGGATTCTCTTGACACCGCTGAGCGGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCAt < 2:457087/143‑1 (MQ=255)
aacaaTGCTTCTTGCGTTGAAGACCTGGGCGCGCATGCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTa < 1:535375/143‑1 (MQ=255)
ttcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg < 1:168850/143‑1 (MQ=255)
tttCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGCGTACATCAACGGcc > 2:552296/1‑143 (MQ=255)
tCGTTGTAGTCTTGGTCGCGTTTTCTCTTGATACAGCTGAGATGGTAATGGCTCGGGAAGAAGAGGTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccac < 1:337871/143‑1 (MQ=255)
gAAGACCTGGGCGTGGATTCTCTTGACGCCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAGTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGc < 2:563165/143‑1 (MQ=255)
aCCTGGGCGCGGATTCTCTTGACACCGCTGAGCTGGTAATGGCTCAGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTaa > 2:229227/1‑143 (MQ=255)
gATTCTCTTGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAtctc > 2:139424/1‑143 (MQ=255)
ttGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGACCATCTCCAGGCgg > 1:345541/1‑143 (MQ=255)
ttGACACCGCTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCgg > 2:471479/1‑143 (MQ=255)
gTTGAGCTGGTGATGGGTCTGTAAGAGGGTTTTGATACTGTGCTGCCGGACGAAGAAGCTGAGCAAATTACCACCGTTCAGGCTGCCATTGATTACATCTACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGa < 2:453047/143‑1 (MQ=255)
|
AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGA > NC_000913/1151612‑1151877
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
