Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F71 I0 R1 83 59.3 2271602 90.4% 2053528 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,440,791 C→T 32.4% intergenic (‑7/‑267) ydbK ← / → ydbJ putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,440,7910CT32.4% 125.4 / 66.0 106intergenic (‑7/‑267)ydbK/ydbJputative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
Reads supporting (aligned to +/- strand):  ref base C (40/30);  new base T (14/20);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGA  >  NC_000913/1440649‑1440930
                                                                                                                                              |                                                                                                                                            
tCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGacac                                                                                                                                              <  1:800018/143‑1 (MQ=255)
     aaGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTa                                                                                                                                         <  1:436811/143‑1 (MQ=255)
      aGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAGTAGTAATCATGTGACACGCTTAc                                                                                                                                        >  1:101498/1‑143 (MQ=255)
       gCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCTTAGTGGAAAGGGGAGGAATAGGGTAGATGGCGATGACTTGACTGGTGCGTAATGCGACCGGAGCACCCGCGCCCTTACCGTCGATCGTCATCACCTGCCACCCtttaa                                                                                                                                       >  2:806624/1‑140 (MQ=255)
        ccGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACAt                                                                                                                                      >  1:701798/1‑143 (MQ=255)
          gtcgccGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGACCTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTg                                                                                                                                    <  1:374099/139‑1 (MQ=255)
               cGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCGTCAATTGCGCac                                                                                                                               >  2:867154/1‑142 (MQ=255)
                 gCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGGGGAATGCGGGTGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGCCGTCCTTACATTGCGCAAAt                                                                                                                             >  1:41771/1‑143 (MQ=255)
                 gCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAAt                                                                                                                             >  1:625053/1‑143 (MQ=255)
                 gCCAAGGAATCATCCTGTTCTGCCATCGTGAAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAAt                                                                                                                             <  1:1024305/143‑1 (MQ=255)
                      gtcATCAGGCTGTTCTGTCATTGTTGAACTTGGGGTAATAGTGTAGATGGAGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGTCATTACAGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAggg                                                                                                                        <  2:59114/141‑1 (MQ=255)
                      ggCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAAGGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAggg                                                                                                                        <  2:140823/143‑1 (MQ=255)
                             tcttGTTCTGCCGACGTGGGACTGGTGGTAACAGGGTAGATGGCGATAACTTCACTGGTGCTAAATGCGACCGAACCATCCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACg                                                                                                                 <  2:1045138/140‑1 (MQ=255)
                             gCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACg                                                                                                                 >  1:26425/1‑143 (MQ=255)
                                 gTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGACAATAGTAATCATATGACAGCCTTAAATTGCGCAGCGGAGGGGCGCACGagaa                                                                                                             >  1:1045138/1‑140 (MQ=255)
                                 gTTCTGCCATCGTGGAACTGGGGGTAATAGGGGAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAAt                                                                                                             <  2:1131222/143‑1 (MQ=255)
                                 gTTCTGCCATCGTGGAACTGGGGGTAACAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAAt                                                                                                             <  2:199098/143‑1 (MQ=255)
                                  ttCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCCACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATAGCGCAAATGAGGGGCGCACGAAAtt                                                                                                            >  1:610495/1‑143 (MQ=255)
                                     tGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGACACACGAAATtgct                                                                                                         >  2:105679/1‑143 (MQ=255)
                                     tGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACAGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCGTACATTGCGCGAATGAGAAGCGCACGAAAGtgct                                                                                                         >  2:355288/1‑143 (MQ=255)
                                      gCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTGACATTGGGCAAATGAGGGGCGCACGAACGtgctg                                                                                                        >  1:876936/1‑143 (MQ=255)
                                        cATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATCGCGCAAATGAAGGGCGCACGAAATTGCTgcg                                                                                                      >  2:695927/1‑143 (MQ=255)
                                         acctggaaaCTGGGGGGAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgc                                                                                                     <  1:1114576/136‑1 (MQ=255)
                                         aacGTGGGACTGGGGGGACGGGGGTAGCTGGCGAGAACTTCACTGGTGCGAAAGGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCAGATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgc                                                                                                     <  1:299978/141‑1 (MQ=255)
                                         aTCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCGTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgg                                                                                                     >  2:477281/1‑142 (MQ=255)
                                          tCGTGGGACTGGGGGTAATAGGGTAGATGGCGAGAACTTCAGTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgcg                                                                                                    <  2:867802/143‑1 (MQ=255)
                                          tCGTGGAACTTGGGGTAATAGGCGAGTTATCGATAACTCCACTTGTGCGAAATGCGACCTAAGCAAGCGCGCCATTACCGTCAATTGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgcg                                                                                                    <  2:357761/143‑1 (MQ=255)
                                          tCGTGGAACTGGGGGGAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATAAGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgcg                                                                                                    <  2:457581/143‑1 (MQ=255)
                                             tGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGccc                                                                                                 >  1:834640/1‑143 (MQ=255)
                                               gAACTGGGGGTAATAGGGTAGATGGCGTTAACTTCACTGGTGCAGAGTGCTACGGAACCAATCGCGCCAGTACCGGCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCag                                                                                               <  2:237443/143‑1 (MQ=255)
                                               gAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCag                                                                                               <  1:392324/143‑1 (MQ=255)
                                                 aCTGGGGGTAATAGGGTAGCTGGCGATAACTTCACTGGTGCGACATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagt‑a                                                                                            <  1:984998/143‑1 (MQ=255)
                                                   tGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCGCGAAATTGCTGCGCGCCCagt‑agt                                                                                          >  1:721244/1‑143 (MQ=255)
                                                   tGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagt‑agt                                                                                          >  1:720887/1‑143 (MQ=255)
                                                    gggggTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAgt‑agta                                                                                         <  1:361533/143‑1 (MQ=255)
                                                       ggTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATc                                                                                      <  2:292532/143‑1 (MQ=255)
                                                         ttaTAGGGTAGATGGCGATGACTTCCCTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAAGCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCtt                                                                                    <  2:190041/141‑1 (MQ=255)
                                                          aagtgggTAGATTGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAAGAGTAAGCATATGACATCCTTACATTGCGCAAAGGAGGGGCGCACGGAATTGCTGCGCGCCCAGT‑AGTAATCttt                                                                                   <  1:385370/139‑1 (MQ=255)
                                                            tggggTAGATGGCGATAACTTCACGGGGGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCa                                                                                 <  2:876936/141‑1 (MQ=255)
                                                            tAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCa                                                                                 <  1:330534/143‑1 (MQ=255)
                                                               ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCACATGAGGGGCGCACGCAATTGCTGCGCGCCCCCT‑AGTCATCTTTCACtt                                                                              >  1:840165/1‑143 (MQ=255)
                                                               ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAAtt                                                                              >  1:584095/1‑143 (MQ=255)
                                                                gTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGAGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAAttt                                                                             <  2:696808/143‑1 (MQ=255)
                                                                  acagcgagATAATTTTGCTGGTGCGAAGTGCGACCGAAGCAACCGCGCCATTTCTTTTAATAGTTATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTa                                                                           <  2:905888/136‑1 (MQ=255)
                                                                  aGATGGCGATAACTTCACTGGGGCGACATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTa                                                                           <  2:360674/143‑1 (MQ=255)
                                                                    aTGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCCATAGTAATCATATGACACCCTTACATTGCGCAAATGACGGGCGCACGAAATTGCTTCGCGCCCAGT‑AGTAACCTTTCAATTTTCGc                                                                         >  1:934563/1‑143 (MQ=255)
                                                                    aTGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGc                                                                         >  1:911064/1‑143 (MQ=255)
                                                                    aTGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATCGCTGCGCGCCCAGT‑AGCAACATTGCAATTGTAGc                                                                         >  2:926808/1‑143 (MQ=255)
                                                                       gCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCaaa                                                                      >  1:767976/1‑143 (MQ=255)
                                                                           tagcTTCACTGGTGCGATCGGCGACCGAAGCAACCGCACCATGACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGc                                                                  <  2:67061/140‑1 (MQ=255)
                                                                            aaCTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCt                                                                 <  1:366556/143‑1 (MQ=255)
                                                                              cTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCACATGAGGGGCGCACGAAATTCCTGCGCGCCCAGT‑AGTAACCTTTCCATGTTAGCAAATGGCttt                                                               >  2:653775/1‑143 (MQ=255)
                                                                               ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACCTACTTACATTGCGCACCTGACCGCTGCTCCAAAGTGCTGCCCGCCCAGT‑AGTAGTCTTTCAATTTTAGCAAGTGGCtttg                                                              >  1:257752/1‑142 (MQ=255)
                                                                               ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTttc                                                              >  1:774560/1‑143 (MQ=255)
                                                                               ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTACGCAAATGAGGGGCGCACGAAATTGCTGCGCGCACAGT‑AGCACTCTGTGAAGTTTAGCAACTGGCTttc                                                              >  2:771428/1‑143 (MQ=255)
                                                                                   cTGGTGCTAAATGCGACCGAAGTAACTGTGTCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTtcttct                                                          <  1:664894/143‑1 (MQ=255)
                                                                                   cTGGTGAGCAATGCGACCGACGCAGCGGGGCCATTCCCGGCAATTGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGTGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTtcttct                                                          <  2:45782/143‑1 (MQ=255)
                                                                                    tGGTGCGAAATGCGTCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCCTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTg                                                         <  2:286872/143‑1 (MQ=255)
                                                                                    tGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAGTGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTGTCAATTTTAGCAAATGGCCTTCTTATg                                                         >  2:613564/1‑143 (MQ=255)
                                                                                      gTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATCAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTACTCTTTCAATTTTACCAAATGCCTTTCTTCTCCa                                                       >  2:482596/1‑143 (MQ=255)
                                                                                         cGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCACGTGGCTTTCTTCTGCAttt                                                    >  1:886941/1‑143 (MQ=255)
                                                                                           aaaTGCTACCGATGCATCCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTc                                                  <  1:1087373/143‑1 (MQ=255)
                                                                                           aaaTGCGACTGAAGCAACCGCGCCATTACCGTCAATAGTAAGAAGATGACACCCCTACATTGCGCACAGGAGGGGCGCACGAAATTGCTCCGCGCCCAGT‑AGTAATCGTTAAATTTTAGCAAATGGCTTCCTTCTGCagtctc                                                  >  2:1043174/1‑138 (MQ=255)
                                                                                               gCGACCGAAGCAACCGCGCCATTGCCGTCAATAGGAATGCTGTGACATCCTTACATTGCGCAAGTGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATGTTAGCAAATGGCTTTCTTCTGCATTTTCGCtt                                              <  2:390208/143‑1 (MQ=255)
                                                                                                cGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCttt                                             <  2:721244/143‑1 (MQ=255)
                                                                                                 gACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCACTTGGCTTTCTTCTGCTTTTTCGCtttt                                            >  1:185077/1‑143 (MQ=255)
                                                                                                 gACCGAAGCAAACGCGCCATTACCGTCAATAGTAATCATATAACACCCTTCCATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAACCTTTCAATTTTAGCAAATGGACTTCTTCTGCATTTGCGCtgtt                                            >  2:346607/1‑143 (MQ=255)
                                                                                                  aCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTGGTCGCACAACGCAAGCAAATGGCTTTCTTCTGCATTTTCGCTTTtg                                           >  1:253595/1‑143 (MQ=255)
                                                                                                  aCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCCGCATTTTCGCCTTtg                                           >  1:144264/1‑143 (MQ=255)
                                                                                                  aCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCCGCATTTCCGCttttc                                           >  2:343114/1‑142 (MQ=255)
                                                                                                       aGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCACATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTgtgtgc                                      >  1:416304/1‑141 (MQ=255)
                                                                                                       aGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTcc                                      >  1:448035/1‑143 (MQ=255)
                                                                                                         taaCCGCGCCATTACCGTCAGTAGTGATCATATGACACCCTTACATGGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTcccc                                    <  2:149057/142‑1 (MQ=255)
                                                                                                         cAACCGCGCCATTACCGTCAACAGTAATCATATGACATCCTTACATTGCGCAAATCAGGGGCGCACGAAATTGCGGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTcccc                                    <  1:1012569/143‑1 (MQ=255)
                                                                                                           aCCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCca                                  >  1:1131380/1‑143 (MQ=255)
                                                                                                             cgcgCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAATTGAGGGGCGCACGAAATTGCAATAAACCCAGTCATCCATC‑CTCAGATTTATCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCccca                                >  2:1102545/1‑143 (MQ=255)
                                                                                                              gcgcCATTACCGTCAATAGTAATCATATGACATCCTTACATTAAGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGG‑ATTAATCTTTCAATTTTCGCAAATGGCTTCCTTCTGCATTTTCGCTTTTGTGTCCCGCACAt                               >  2:281492/1‑143 (MQ=255)
                                                                                                              gcgcCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACAt                               >  1:810793/1‑143 (MQ=255)
                                                                                                                  cATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAgg                           >  1:1023747/1‑142 (MQ=255)
                                                                                                                  cATTACCGTCAATAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGc                           <  1:1112316/143‑1 (MQ=255)
                                                                                                                  cATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGc                           <  2:625053/143‑1 (MQ=255)
                                                                                                                   aTTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagt‑a                                                                                            <  1:709168/77‑1 (MQ=255)
                                                                                                                   aTTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagt‑a                                                                                            >  2:709168/1‑77 (MQ=255)
                                                                                                                   aTTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCg                          >  1:626650/1‑143 (MQ=255)
                                                                                                                    ttACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGt                         <  2:834640/143‑1 (MQ=255)
                                                                                                                        cGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCATTTTTAGCACATGGCTTTCTTCTGCATTTTCGATTTTGTGTCCCCCACATCAGCGTaatg                     >  1:61455/1‑143 (MQ=255)
                                                                                                                         gTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatga                    >  1:503149/1‑143 (MQ=255)
                                                                                                                          tCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgac                   >  2:890583/1‑142 (MQ=255)
                                                                                                                           cAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaat                  <  1:223241/143‑1 (MQ=255)
                                                                                                                            aaTAGTAATCATATGACATCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaatg                 >  1:538635/1‑143 (MQ=255)
                                                                                                                            aaTAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACCTCAGCGTaatgaatg                 >  1:821161/1‑143 (MQ=255)
                                                                                                                             aTAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGCAAAGAATGt                >  2:532638/1‑143 (MQ=255)
                                                                                                                               aGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTACGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGATTTTGTGTACCCCACCTCCGTGTAATGAATGttt              >  2:343687/1‑143 (MQ=255)
                                                                                                                                gtgaTCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCACT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt             <  2:130281/140‑1 (MQ=255)
                                                                                                                                gTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt             <  1:1012084/143‑1 (MQ=255)
                                                                                                                                ctAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt             <  1:785883/142‑1 (MQ=255)
                                                                                                                                     cATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATcc        >  1:187650/1‑142 (MQ=255)
                                                                                                                                           acatCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACaga  <  1:20487/143‑1 (MQ=255)
                                                                                                                                           acacCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCCGT‑AGTAATCTTTCAATTTTAGCAACTGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACaga  >  1:447907/1‑143 (MQ=255)
                                                                                                                                           acacCCTTACAGTGAGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACaga  <  1:910729/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
TCTTTAAGCCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGT‑AGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGA  >  NC_000913/1440649‑1440930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A