Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F83 I0 R1	128	58.1	2217184	91.0%	2017637	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,500,114	G→A	20.4%	S112S (AGC→AGT)	ydcK ←	putative enzyme YdcK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,500,114	0	G	A	20.4%	100.1 / 18.6	54	S112S (AGC→AGT)	ydcK	putative enzyme YdcK
Reads supporting (aligned to +/- strand): ref base G (19/23); new base A (5/6); total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAA  >  NC_000913/1499972‑1500250

cccTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACg                                                                                                                                          <  1:665987/143‑1 (MQ=255)

 ccTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATAGGCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGc                                                                                                                                         >  1:1046194/1‑143 (MQ=255)

      cgcgATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCa                                                                                                                                    >  1:765332/1‑143 (MQ=255)

         gATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGAGACACTGTCACta                                                                                                                                 >  2:358648/1‑142 (MQ=255)

              tAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTtatata                                                                                                                            >  1:1067065/1‑143 (MQ=255)

               aaaTTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTatatat                                                                                                                           <  1:1001675/143‑1 (MQ=255)

                 cttgggAGCAGGAGTTGGTGTTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGc                                                                                                                         <  2:614879/139‑1 (MQ=255)

                      gAGCAGGGGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCt                                                                                                                    <  1:242085/143‑1 (MQ=255)

                        gCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTgg                                                                                                                  >  1:236973/1‑143 (MQ=255)

                           ggAGTTCATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTg                                                                                                               <  1:216286/143‑1 (MQ=255)

                               ttGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGAttt                                                                                                           >  2:635652/1‑143 (MQ=255)

                                 aaggggcGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCa                                                                                                         <  1:502197/137‑1 (MQ=255)

                                  aTGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGCCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCAc                                                                                                        <  1:608035/143‑1 (MQ=255)

                                      tCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTCATATATGCACCCTGGCTGATGTCACtatt                                                                                                    >  2:395608/1‑143 (MQ=255)

                                      gcGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATAAAATAAGTCTTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACtatt                                                                                                    <  1:754034/142‑1 (MQ=255)

                                          gCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCAGCCGTGCTTGTTTCACTATTATCg                                                                                                >  2:718036/1‑143 (MQ=255)

                                             ctAAGCCTTGTGCGGCCACGATCATAGAATGTTGACCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATc                                                                                             <  2:820886/142‑1 (MQ=255)

                                                     tgtgCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGtt                                                                                     >  1:611526/1‑143 (MQ=255)

                                                         cggcgACGATCATAGAATGTTGATCAATTAAGGCGAGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATcc                                                                                 <  1:423552/143‑1 (MQ=255)

                                                          ggcgACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCg                                                                                >  1:1009448/1‑143 (MQ=255)

                                                           tgtacgaTCATAGAATGTTGATCAATTGAGGCGTGACCAAAGATTCTACAGTGCCCGTAAACTAGTGATTCATGAATGGTGACGCTGTCACGTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGt                                                                               <  1:348276/140‑1 (MQ=255)

                                                            cgacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGtt                                                                              >  2:766682/1‑143 (MQ=255)

                                                            cgacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGtt                                                                              >  2:820217/1‑143 (MQ=255)

                                                            cgacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGtt                                                                              <  1:453010/143‑1 (MQ=255)

                                                             gacgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTg                                                                             <  1:637124/143‑1 (MQ=255)

                                                              acgaTCATAGTATGTTGATCAATTCAGGTGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGCCACTCCAATCTTCATCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGc                                                                            <  2:237545/143‑1 (MQ=255)

                                                              acgaTCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGc                                                                            <  1:929743/143‑1 (MQ=255)

                                                                 attatgtaaTGTTGATCAATTAAGGCGTCACGAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTa                                                                         <  2:794430/136‑1 (MQ=255)

                                                                    aTAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAc                                                                      <  2:257174/143‑1 (MQ=255)

                                                                    aTAGAATGTTGAGCAAGTAAGGCGTGACCAAATATGCTACACTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATCTATGCACCCTGGCTGTTTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAAc                                                                      <  1:210363/143‑1 (MQ=255)

                                                                        aaTGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACGTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACctct                                                                  <  2:742403/143‑1 (MQ=255)

                                                                           gTTGATCGATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTccc                                                               <  2:278589/143‑1 (MQ=255)

                                                                           gTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACATCTccc                                                               >  1:721735/1‑143 (MQ=255)

                                                                             ggATCAATTAGGGCGTGACCAAATATTCTACACTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCca                                                             <  2:36737/142‑1 (MQ=255)

                                                                              gATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcac                                                            >  2:751449/1‑143 (MQ=255)

                                                                               aTCGATTAAGGCCTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcaca                                                           >  1:570176/1‑143 (MQ=255)

                                                                               aTCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCcaca                                                           <  2:765332/143‑1 (MQ=255)

                                                                                tCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAACGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAg                                                          <  1:167406/143‑1 (MQ=255)

                                                                                   aTTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGccc                                                       >  2:746488/1‑141 (MQ=255)

                                                                                      aaGGCGTGACCAAATATTCGACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCt                                                    <  2:1029302/143‑1 (MQ=255)

                                                                                         gCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCCCCGCCCGCTgtt                                                 >  2:366069/1‑141 (MQ=255)

                                                                                         gCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATAGATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTCGCGTAAACCTCTCCCCACAGCACGCTAGt                                                 >  2:677326/1‑143 (MQ=255)

                                                                                                cAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCCCGCTGGTGCCGGTg                                          >  1:192425/1‑143 (MQ=255)

                                                                                                 aaaTATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGa                                         <  2:236973/143‑1 (MQ=255)

                                                                                                              tGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATGCCGGCGTTATACCTCGCGTAAGCCTCTCCCCACAGCACGCTGGTGCAGGTGATACGCGTATTAcg                            >  1:206939/1‑142 (MQ=255)

                                                                                                              tGCCCGTAAACTAATGACACCAGCATGTTGACCCGGTCAATTAGAGGTGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTAcc                            <  1:983565/143‑1 (MQ=255)

                                                                                                               gCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTCTCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCg                           <  2:166088/143‑1 (MQ=255)

                                                                                                                cccGTAAACTAATGAGTCATGAATGGTGACGCTGGCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGGGATAAGCGTATTACCgg                          >  2:799100/1‑143 (MQ=255)

                                                                                                                      aaCTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCACTCCTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCa                    <  1:695984/143‑1 (MQ=255)

                                                                                                                       aCTAATGTGTCATGAATGGTGACACTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCAc                   <  1:103207/143‑1 (MQ=255)

                                                                                                                         tAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGTGTAAACCTCTCCCCACAGCACGATGGTGCCGGTGATACGCGTATTACCAGAAaccacca                 >  2:545005/1‑142 (MQ=255)

                                                                                                                           aTGAGTCATGAATGGTGACGCTGCCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTCATCCGTTGCGTAAACCTCTCCCCAAACCACGCCGGTGCCACCGACACGCGAACTACCGGAAAACAccccc               >  2:1015894/1‑140 (MQ=255)

                                                                                                                                   tGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCTCAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGaa       <  1:635652/143‑1 (MQ=255)

                                                                                                                                     taTGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACg     >  2:576119/2‑143 (MQ=255)

                                                                                                                                        ggTGACGCTGTGACTGATACATGCAGGCTGGCTGCTTGCGCTATTATCGATGCAGACGTTAGCCGTTGCGTATGCGTGTCCCCTAAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCaa  <  1:304213/143‑1 (MQ=255)

CCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCCCGTAAACTAATGAGTCATGAATGGTGACGCTGTCACTTATATATGCACCCTGGCTGATTTCACTATTATCGATCCAGACGTTATCCGTTGCGTAAACCTCTCCCCACAGCACGCTGGTGCCGGTGATACGCGTATTACCGGAAATCACCGCTCCGCCGAACGCAA  >  NC_000913/1499972‑1500250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A