Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F97 I0 R1	51	42.6	1617048	90.7%	1466662	142.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,159,079	G→T	100%	G197V (GGT→GTT)	oxyR →	DNA‑binding transcriptional dual regulator OxyR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,159,079	0	G	T	100.0%	166.7 / ‑6.7	53	G197V (GGT→GTT)	oxyR	DNA‑binding transcriptional dual regulator OxyR
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (26/25); total (27/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGG  >  NC_000913/4158946‑4159219

gAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGttt                                                                                                                                     <  1:580878/143‑1 (MQ=255)

gAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGttt                                                                                                                                     <  1:270102/143‑1 (MQ=255)

       tcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTTGCTAGCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGcgcggt                                                                                                                              >  1:377896/1‑141 (MQ=255)

        cattGAAGGGCCGTTGTTTGATGAGCCAATGTTGCTTGCTATCTATGTAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATc                                                                                                                             <  2:253681/143‑1 (MQ=255)

        cagtGATGTGCCGTGGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATc                                                                                                                             <  2:252966/140‑1 (MQ=255)

         attGAAGTGTCGTTGTGTGAGTAGCTGATGGTGGTGGCTATCTATGGAGATGACCCGTGGGCGAACCGCGAATGCGTTCCGAGGGCCGATCTTGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCa                                                                                                                            <  2:599375/143‑1 (MQ=255)

               gTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAAt                                                                                                                      >  1:512701/1‑143 (MQ=255)

                   cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAACAACTGCTGATGCTGGAAGATGTTTACTGTTTGCGCGATCAGACAATGGGt                                                                                                                  >  1:409853/1‑143 (MQ=255)

                   cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTATACTGTATGAGCGAACAAGCAATggga                                                                                                                  >  1:410403/1‑142 (MQ=255)

                    gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGACGATGTTCACTGTTTGCGCGGTCAGGCAATGGGtt                                                                                                                 >  1:126273/1‑143 (MQ=255)

                     ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGTGCGATCAGGCAATGGGttt                                                                                                                <  1:535873/143‑1 (MQ=255)

                           gatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCCCTGTTTGCGCGCTCAGGCAATGGGTGGCTGttt                                                                                                          >  1:190017/1‑143 (MQ=255)

                           gatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGGTTCTGttt                                                                                                          >  1:421709/1‑143 (MQ=255)

                               aGCCAATGTTGCTGGCTATCGATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa                                                                                                      <  1:103023/143‑1 (MQ=255)

                                ggcgaggTTGCTGGGTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg                                                                                                     <  2:121300/137‑1 (MQ=255)

                                gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAg                                                                                                     <  1:39542/143‑1 (MQ=255)

                                gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGAAATGGGATTCTGTTTTGAAg                                                                                                     >  1:273691/1‑143 (MQ=255)

                                 ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGGTTCTGTTTTGAAGc                                                                                                    >  1:310679/1‑143 (MQ=255)

                                   aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                                                  <  1:510415/143‑1 (MQ=255)

                                    aTGTTGCTGGCCATGTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg                                                                                                 <  1:378522/143‑1 (MQ=255)

                                     tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTTCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                                                >  1:272101/1‑143 (MQ=255)

                                       ttGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                                              >  1:466193/1‑143 (MQ=255)

                                              ctatctatGAAGATCACCCGTAGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGaa                                                                                       <  1:211963/143‑1 (MQ=255)

                                               tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAg                                                                                      <  1:305645/143‑1 (MQ=255)

                                                ctctatGCAGAGCACCTGCGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGa                                                                                     <  2:40426/142‑1 (MQ=255)

                                                    atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                                                 >  1:199832/1‑143 (MQ=255)

                                                    atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGGTTTGAAGCCGGGGCGGATGAAGCTaca                                                                                 >  1:44896/1‑143 (MQ=255)

                                                        aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACt                                                                             >  1:555964/1‑143 (MQ=255)

                                                           ccACCCGTGGGCGAACCGCGCATGCGTACCGATGGCCGATCTGGCAGGGGAAACACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                                          <  1:658261/142‑1 (MQ=255)

                                                               ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGGTCTGGCAGGGGAAAAACTGCTGATGCTGATAGGTGGTCGCTGTTTGCGCGATCAGGCAACGGGGTTCTGTTTTGAGGCCGGGGCGGCTGAAGATGCACACTTCcgcgc                                                                      >  2:12213/1‑143 (MQ=255)

                                                                  tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAc                                                                   >  1:141310/1‑143 (MQ=255)

                                                                     gCGAACCGCGCATGCGGACCGCTGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                                                <  2:613820/143‑1 (MQ=255)

                                                                     gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGGTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                                                >  2:254955/1‑143 (MQ=255)

                                                                           cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                          >  1:781047/1‑143 (MQ=255)

                                                                            gcgGATGTGGACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCGTGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                                         <  2:643261/143‑1 (MQ=255)

                                                                                   cGTACCGATGGCCGATCTGGCAGGGGAAAAACTTCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                  <  2:272101/143‑1 (MQ=255)

                                                                                    ggaCCGATGGTCGATCGGGCCGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                 <  2:670039/141‑1 (MQ=255)

                                                                                             gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggt                                        >  1:578132/1‑143 (MQ=255)

                                                                                             gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggt                                        <  1:265617/143‑1 (MQ=255)

                                                                                               cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGCGGGATGACGGTACACACTTCCGCGCGACCCGCCTGGAAAATCGGCGCGGCAtggtgg                                      >  2:645148/1‑143 (MQ=255)

                                                                                                     ggCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTCCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                <  1:405979/143‑1 (MQ=255)

                                                                                                     ggCAGGGGAAAAACTGCTGATGCTGGAAGATGTGCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATGCACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                <  1:767112/143‑1 (MQ=255)

                                                                                                      gCAGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCACCATGGTGGCGGCAgg                               >  1:477516/1‑143 (MQ=255)

                                                                                                        aGGGGAAAAACTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGCTGAAGATACACACTTCCGCGCGACCAGCCTGGGAACTCTGCGCAACATGGTGGCGGCAGGtc                             >  2:727507/1‑142 (MQ=255)

                                                                                                               aagctgctgATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                      <  1:704233/140‑1 (MQ=255)

                                                                                                               aaaCTGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACCCTGCGCGACATGGGGGCGGCCGGTAGCGGGAt                      >  2:379874/1‑143 (MQ=255)

                                                                                                                    gccgatgatGGAAGATGTTCACCGTTTGCGCGATCAGGCAATGGGTTGCTGTTTTGAAGCCGGGGCGGATGAAGATACACCCTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                 <  1:175143/140‑1 (MQ=255)

                                                                                                                     ctgATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACttt                >  1:11230/1‑143 (MQ=255)

                                                                                                                          gCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                                                               >  1:757188/1‑75 (MQ=255)

                                                                                                                          gCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                                                               <  2:757188/75‑1 (MQ=255)

                                                                                                                             ggAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTAGCGGCAGGTAGCGGGAACACTTTACTACCAg        >  1:268336/1‑143 (MQ=255)

                                                                                                                               aaGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTCCTGCCAgcg      >  2:438290/1‑143 (MQ=255)

                                                                                                                                   tGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg  <  2:618326/143‑1 (MQ=255)

GAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGG  >  NC_000913/4158946‑4159219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A