breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
RA 151,519 A→C 9.3% I27M (ATT→ATG yadK ← putative fimbrial protein YadK
RA 151,522 G→T 8.8% A26A (GCC→GCA yadK ← putative fimbrial protein YadK
RA 231,007 C→A 8.2% intergenic (‑126/‑115) yafC ← / → yafD putative LysR family transcriptional regulator YafC/endonuclease/exonuclease/phosphatase domain‑containing protein YafD
RA 231,008 T→G 8.4% intergenic (‑127/‑114) yafC ← / → yafD putative LysR family transcriptional regulator YafC/endonuclease/exonuclease/phosphatase domain‑containing protein YafD
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 285,882 T→G 10.7% I163S (ATC→AGC)  yagG → putative D‑xylonate transporter YagG
RA 449,135 C→A 100% V503L (GTA→TTA)  cyoB ← cytochrome bo3 ubiquinol oxidase subunit 1
RA 454,891 G→T 100% intergenic (+102/‑242) bolA → / → tig DNA‑binding transcriptional dual regulator BolA/trigger factor
RA 461,010 C→A 100% T708N (ACT→AAT)  lon → Lon protease
RA 481,225 T→G 7.7% intergenic (‑517/+29) tomB ← / ← acrB protein that modulates Hha toxicity/multidrug efflux pump RND permease AcrB
RA 595,519 T→A 7.9% intergenic (‑76/‑81) cusR ← / → cusC DNA‑binding transcriptional activator CusR/copper/silver export system outer membrane channel
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G 100% L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,597,272 T→A 9.2% pseudogene (815/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,801,361 C→A 100% V404F (GTC→TTC)  thrS ← threonine‑‑tRNA ligase
RA 1,837,091 T→C 8.8% V340A (GTT→GCT)  ynjB → putative ABC transporter periplasmic binding protein YnjB
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 1,987,442 Δ1 bp 9.8% intergenic (+14/+65) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,987,450:1 +A 8.0% intergenic (+22/+57) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C 100% A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,392,235 G→T 100% Y269* (TAC→TAA nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,653,104 G→C 10.4% W204S (TGG→TCG) ‡ sseA → 3‑mercaptopyruvate sulfurtransferase
RA 2,653,105 G→C 8.7% W204C (TGG→TGC) ‡ sseA → 3‑mercaptopyruvate sulfurtransferase
RA 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,800,515 C→A 7.8% intergenic (+40/‑208) ygaM → / → nrdH DUF883 domain‑containing protein YgaM/glutaredoxin‑like protein
RA 2,800,517 T→A 7.3% intergenic (+42/‑206) ygaM → / → nrdH DUF883 domain‑containing protein YgaM/glutaredoxin‑like protein
RA 2,800,519 T→G 6.7% intergenic (+44/‑204) ygaM → / → nrdH DUF883 domain‑containing protein YgaM/glutaredoxin‑like protein
RA 3,041,634 A→C 100% T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,334,704 G→C 10.4% intergenic (+23/‑205) ispB → / → sfsB all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB
RA 3,512,306 G→C 8.8% I13M (ATC→ATG yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,938,255 T→A 7.2% L10Q (CTG→CAG)  rbsR → DNA‑binding transcriptional dual regulator RbsR
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,033,565 G→A 100% G141R (GGG→AGG)  trkH → K(+) transporter TrkH
RA 4,056,531 A→G 7.1% intergenic (‑192/+94) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,539 T→A 6.4% intergenic (‑200/+86) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,540 T→A 6.4% intergenic (‑201/+85) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,159,079 G→T 100% G197V (GGT→GTT)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,203 C→T 100% intergenic (‑104/+129) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,060 C→T 26.7% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,551,422 T→A 9.4% intergenic (‑126/‑214) gntP ← / → uxuA fructuronate transporter/D‑mannonate dehydratase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315646NA (NA)9 (0.230) 8/254 NT NA noncoding (418/1255 nt) IS3 repeat region
?NC_000913 = 315665 NA (NA)noncoding (437/1255 nt) IS3 repeat region
* ? NC_000913 315843 =NA (NA)3 (0.070) 3/258 NT NA noncoding (615/1255 nt) IS3 repeat region
?NC_000913 315886 = NA (NA)noncoding (658/1255 nt) IS3 repeat region
* ? NC_000913 392478 =NA (NA)7 (0.170) 6/260 NT NA noncoding (489/1258 nt) IS3 repeat region
?NC_000913 392552 = NA (NA)noncoding (415/1258 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1854 (45.540) 248/262 NT 97.6% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 46 (1.120)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 882488 =38 (0.930)3 (0.080) 3/254 NT 7.4% coding (247/759 nt) deoR DNA‑binding transcriptional repressor DeoR
?NC_000913 882517 = 38 (0.960)coding (218/759 nt) deoR DNA‑binding transcriptional repressor DeoR
* ? NC_000913 1207790 =6 (0.150)26 (0.720) 26/232 NT 83.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 5 (0.140)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078056 (0.150)16 (0.440) 14/232 NT 75.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 5 (0.140)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 146343756 (1.360)6 (0.150) 5/262 NT 9.7% coding (1313/1314 nt) paaK phenylacetate‑CoA ligase
?NC_000913 = 1463490 56 (1.380)intergenic (+52/‑49) paaK/paaX phenylacetate‑CoA ligase/DNA‑binding transcriptional repressor PaaX
* ? NC_000913 2422747 =45 (1.100)3 (0.080) 3/250 NT 6.8% coding (793/891 nt) rpnB recombination‑promoting nuclease RpnB
?NC_000913 2422773 = 39 (1.000)coding (767/891 nt) rpnB recombination‑promoting nuclease RpnB
* ? NC_000913 = 270007741 (1.000)3 (0.080) 3/240 NT 7.1% intergenic (+154/+40) yfhL/shoB putative 4Fe‑4S cluster‑containing protein YfhL/toxic peptide ShoB
?NC_000913 = 2700087 41 (1.100)intergenic (+164/+30) yfhL/shoB putative 4Fe‑4S cluster‑containing protein YfhL/toxic peptide ShoB
* ? NC_000913 3603658 =48 (1.170)3 (0.080) 3/250 NT 6.4% coding (586/1494 nt) ftsY signal recognition particle receptor
?NC_000913 3603682 = 43 (1.110)coding (562/1494 nt) ftsY signal recognition particle receptor
* ? NC_000913 = 3620220NA (NA)3 (0.080) 3/252 NT 100% coding (1029/4236 nt) rhsB rhs element protein RhsB
?NC_000913 = 3620255 0 (0.000)coding (1064/4236 nt) rhsB rhs element protein RhsB
* ? NC_000913 4323298 =NA (NA)3 (0.080) 3/250 NT NA noncoding (1/36 nt) other REP324b
?NC_000913 4323328 = NA (NA)noncoding (31/36 nt) other REP324b
* ? NC_000913 = 433812335 (0.850)3 (0.080) 3/252 NT 7.9% intergenic (‑194/+131) adiY/adiA DNA‑binding transcriptional activator AdiY/arginine decarboxylase, degradative
?NC_000913 = 4338130 37 (0.950)intergenic (‑201/+124) adiY/adiA DNA‑binding transcriptional activator AdiY/arginine decarboxylase, degradative
* ? NC_000913 = 438365751 (1.240)3 (0.080) 3/246 NT 5.7% intergenic (+37/‑182) epmA/yjeM EF‑P‑lysine lysyltransferase/putative transporter YjeM
?NC_000913 = 4383664 51 (1.330)intergenic (+44/‑175) epmA/yjeM EF‑P‑lysine lysyltransferase/putative transporter YjeM