Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F83 I1 R1 24 40.7 1567444 90.4% 1416969 141.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,640,231 C→A E26* (GAA→TAA)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,640,2310CA100.0% 149.0 / NA 47E26* (GAA→TAA) arcADNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/28);  total (19/28)

GCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCC  >  NC_000913/4640097‑4640356
                                                                                                                                      |                                                                                                                             
gcgcAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCAGAGTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCGTCGAAAACATCATAGCCTTACGCTTCGa                                                                                                                       <  2:180753/143‑1 (MQ=255)
     gTTCACGCGCTAAGATAAGACCGTTCTTACCCGGCAGGCTGAGATCCACGATCACCAGGTGGCGGTCACGATCAGAGAGGACCTAATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATa                                                                                                                  <  2:636830/143‑1 (MQ=255)
        cACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACAAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCAGCTGTCGCATCGAAAAGATCATAGCATTACGCGCCGACAATACtc                                                                                                               >  2:574816/1‑142 (MQ=255)
         aCGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACttt                                                                                                              <  1:575891/143‑1 (MQ=255)
              cTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAAc                                                                                                         <  1:274469/143‑1 (MQ=255)
                aaCAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgt                                                                                                       <  2:33863/143‑1 (MQ=255)
                       gaCCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCgt                                                                                                <  1:685678/143‑1 (MQ=255)
                       gaCCGTTCTCACCCGGCAGATTGATATCCATGCTCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCgt                                                                                                <  2:739806/143‑1 (MQ=255)
                              cTTACCCGACAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCa                                                                                         >  1:134818/1‑143 (MQ=255)
                                  cccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:514525/143‑1 (MQ=255)
                                  cccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:781396/143‑1 (MQ=255)
                                  cccGGCAGATTGAGATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTc                                                                                     <  1:56275/143‑1 (MQ=255)
                                   ccGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGCAAATACTTTTCAACGTGTTGCGTGTTACCAACTCg                                                                                    >  2:215075/1‑143 (MQ=255)
                                   ccGGCAGATTGATATCCATGATCAACAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCTGCGCCCTCTGTCGCTTCGCCAACATCCTAGCCTTACGCTTAGAAAATAATTTTCAACGTGTTAAGTGTTACAAACTCg                                                                                    >  1:30870/1‑143 (MQ=255)
                                        aGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCACCTAGGCTTc                                                                               >  2:663716/1‑143 (MQ=255)
                                           tagATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAc                                                                            <  2:332443/141‑1 (MQ=255)
                                             gATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGa                                                                          >  2:10232/1‑143 (MQ=255)
                                                 tCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAg                                                                      >  1:715120/1‑143 (MQ=255)
                                                          aCCAGGTTGATGTCATATTCAGAGAGGATCCGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCGTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGCCTTCAACGATAAGAATGTGCgg                                                             <  2:350039/143‑1 (MQ=255)
                                                             aGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCCTCTGTCGCTTCGCAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTCGCGTGCTACCAACACGTCTTCCACGATAAGCATGCGCGGAGt                                                          >  2:694177/1‑143 (MQ=255)
                                                                ttGATGTCATAGTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGACAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTGGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTg                                                       <  2:529728/143‑1 (MQ=255)
                                                                     gTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtgt                                                                                                     >  2:569563/1‑92 (MQ=255)
                                                                     gTCATAAGCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACgtgt                                                                                                     <  1:569563/92‑1 (MQ=255)
                                                                       cagatTCAGGGAGTGTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGGTAAGAATGTGCGGGGTCTGCATGttt                                                <  1:678600/140‑1 (MQ=255)
                                                                          atTAAGAGAAGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCt                                             <  1:380961/143‑1 (MQ=255)
                                                                               gagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCCACGTGTTGCGTGTTACCAACTCGTCTTCCACGATAGGGATGTGCGGGGTGTGCAGGATTGCTAccaa                                        >  2:488422/1‑141 (MQ=255)
                                                                               gagagGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTa                                        <  1:541148/143‑1 (MQ=255)
                                                                                  agGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAAt                                     >  1:191391/1‑143 (MQ=255)
                                                                                     aTCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGc                                  <  2:134818/143‑1 (MQ=255)
                                                                                      tctgtgccATTCCCTCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGcc                                 <  2:184969/136‑1 (MQ=255)
                                                                                      tCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCCACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATCTTTGCTACCTaaacggcc                                 >  1:36714/1‑138 (MQ=255)
                                                                                      gcTGGTGCATTTCCGCGTCGTCTGTCGCTTCGAAGACATCATAGCCTTACGCTTCGAAAATACTTGTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGcc                                 <  2:667152/142‑1 (MQ=255)
                                                                                        tGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCaa                               <  1:368602/143‑1 (MQ=255)
                                                                                         gATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACGTAAATTGGCAAc                              >  1:437581/1‑143 (MQ=255)
                                                                                             cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaaa                          <  1:258992/143‑1 (MQ=255)
                                                                                             cATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTaaa                          <  2:59525/143‑1 (MQ=255)
                                                                                                   cgcgCCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCCATGGCAAGAAGGGGTGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGaaa                    >  1:775191/1‑143 (MQ=255)
                                                                                                    gcgcCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCCACTACATCgtgcc                   >  1:69715/1‑139 (MQ=255)
                                                                                                      gcCATCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAg                 >  1:627069/1‑143 (MQ=255)
                                                                                                         gtCTGTCTCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGaa              <  2:488530/142‑1 (MQ=255)
                                                                                                          tCTGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAg             <  1:371344/143‑1 (MQ=255)
                                                                                                            tctCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCGAAATTGCCAACTAAATCGAAACAGGAAGTa           <  1:279219/141‑1 (MQ=255)
                                                                                                            tGTCGCTTCGAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTa           >  1:716812/1‑143 (MQ=255)
                                                                                                              gcgcgTCGAAAACATCATAGCCTTACGCTGCGAAGATACTTTTCAACGTGGTGCGGATCACCACCTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTGTGCTACCTAAATGGCCAACTAAATCGAAACAGGAAGTACa         <  2:586283/142‑1 (MQ=255)
                                                                                                                 cTTCGAAAACATCATAGCCTTACGCTGCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACaaaa      <  2:179572/143‑1 (MQ=255)
                                                                                                                     gAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGGATGTGCGGGGTCTGCATGTTTGCTACCTACATTGCCCACTCAATCGAAACAGGAAGTAAAAAAGTca  >  1:475652/1‑142 (MQ=255)
                                                                                                                     gAAAACATCATAGCCTTACGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAAAGATAAGAATGTGCGGGGACTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTAGAAAAGAcc  >  2:190946/1‑143 (MQ=255)
                                                                                                                                      |                                                                                                                             
GCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTCTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCC  >  NC_000913/4640097‑4640356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A