breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 125,387 C→T Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 419,671 Δ1 bp coding (81/1320 nt) brnQ → branched chain amino acid transporter BrnQ
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 759,250 G→T R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 786,462 (A)5→4 coding (830/1053 nt) aroG → 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
RA 1,764,427 G→A intergenic (‑41/+286) sufA ← / ← rydB iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
MC JC 2,399,711 Δ123 bp coding (333‑455/2727 nt) nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,632 T→A V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,473,614 C→T W156* (TGG→TGA rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,646 +T coding (5/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,731,689 C→T Q187* (CAA→TAA)  xylF → xylose ABC transporter periplasmic binding protein
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,204 G→A intergenic (‑105/+128) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,640,231 C→A E26* (GAA→TAA)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1293040 1307069 14030 43 [0] [0] 18 tdk–[yciU] tdk,insZ,adhE,ychE,insH21,oppA,oppB,oppC,oppD,oppF,[yciU]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)42 (0.990) 38/280 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
* ? NC_000913 316042 =NA (NA)20 (0.490) 11/270 2.8 NA noncoding (814/1255 nt) IS3 repeat region
?NC_000913 316055 = NA (NA)noncoding (827/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1768 (42.040) 264/278 0.0 97.8% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 40 (0.940)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 57247140 (0.940)15 (0.470)
+34 bp
10/212 2.1 39.8% coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
?NC_000913 = 2170235 20 (0.470)noncoding (65/1258 nt) IS3 repeat region
* ? NC_000913 1207790 =2 (0.050)38 (1.010) 31/248 0.2 92.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 4 (0.110)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078052 (0.050)41 (1.090) 37/248 0.1 93.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 4 (0.110)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1307070 =0 (0.000)18 (0.430) 17/278 1.6 100% coding (81/330 nt) yciU DUF440 domain‑containing protein YciU
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region