Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A29 F18 I0 R1
|
22 |
15.5 |
612066 |
86.8% |
531273 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,240,359 |
T→A |
23.6% |
I12N (ATC→AAC) |
psiE → |
putative phosphate starvation‑inducible protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,240,359 | 0 | T | A | 23.6%
| 24.5
/ 4.7
| 17 | I12N (ATC→AAC) | psiE | putative phosphate starvation‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (6/7); new base A (2/2); total (8/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 |
GCGCGCTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTGGCTGATGTGCTGTTCGCGCCAGAACAAACCAGCAAA > NC_000913/4240221‑4240486
|
gcgcgcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGCCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTcc > 1:184318/1‑143 (MQ=255)
gcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGATAAACTccacgc < 1:224070/143‑3 (MQ=255)
gcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGATAAACTccacgc < 1:224143/143‑3 (MQ=255)
tGAACAAAACATACACAAAAAATTTAGATCTCCGCCAGATTTTTGCGTTATACAGTAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACTTTACTCTCTCGTCCGCGCGTGTAGTTTATCTCCACCATTTTGCAGACCGTAc < 2:223285/143‑1 (MQ=255)
cAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCaa > 1:283626/1‑143 (MQ=255)
cacgAACAATATAGTTCTCCGGCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCGCTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCct < 2:298196/143‑1 (MQ=255)
aaaaTATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCGCGCGTCCGCGCGGGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCtgt < 1:85929/143‑1 (MQ=255)
tataTCTCCGTTACATTTTTGCTTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTCCTGTGCCTCg < 1:50352/140‑1 (MQ=255)
aGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCaac > 2:81839/1‑142 (MQ=255)
ggAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAg > 1:90367/1‑143 (MQ=255)
gtcACTGTGAAGGAGGTACTTCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTg < 1:215686/141‑1 (MQ=255)
tgtgAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCt < 2:184318/143‑1 (MQ=255)
tgAAGGAGGTACTCCTATGACGTCCCTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTgg < 2:111734/143‑1 (MQ=255)
aCTGCTATGACGTCACTCTCTCGTCCGAGAGTACAGTCGGAACCTAGTATTTTGCAGACCGTACTCAATCTTGTCCTTCTGGTCCGCCGCGAGATGTGGGGGGTCTGCCGTGGCAAAGAATCGGTGCATCTGGCTGATGTGcg > 2:105771/1‑142 (MQ=255)
tGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCCAGGTACCGGTGTATTTGGCTGATGTGCttt > 1:140025/1‑141 (MQ=255)
tcGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAGAGAAACGGTGCATCTGGCTGACGCGCTGTTCGCGCCAGAAcccaccg > 2:280425/1‑137 (MQ=255)
aaatggTGGAGATAAACTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTCCCCCGTCCTTATTTTGACTGTCTTCATCAGCAAAGAAACGGTGCATCTGGATGATGTGCTGTTCGCGCCAGAACACACCAGCaaa > 1:236965/6‑143 (MQ=255)
|
GCGCGCTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTGGCTGATGTGCTGTTCGCGCCAGAACAAACCAGCAAA > NC_000913/4240221‑4240486
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
