breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G 100% L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,499,945 C→T 16.8% A169T (GCA→ACA)  ydcK ← putative enzyme YdcK
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C 100% A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,452,315 C→T 16.3% A8T (GCC→ACC)  yfcS ← putative fimbrial chaperone YfcS
RA 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C 100% T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 92.3% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,240,359 T→A 23.6% I12N (ATC→AAC)  psiE → putative phosphate starvation‑inducible protein
RA 4,296,060 C→T 57.5% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2818145 2818212–2818197 53–68 4 [2] [2] 3 [argY] [argY]
* * ÷ NC_000913 3423765–3424545 3424545 1–781 3 [2] [2] 3 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 316201NA (NA)3 (0.220) 3/246 NT NA noncoding (973/1255 nt) IS3 repeat region
?NC_000913 = 316220 NA (NA)noncoding (992/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)479 (32.450) 193/262 NT 95.5% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 23 (1.550)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =3 (0.200)14 (1.070) 14/232 NT 85.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 2 (0.150)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078053 (0.200)7 (0.540) 7/232 NT 75.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 2 (0.150)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2105826 =14 (0.940)3 (0.210) 3/252 NT 20.4% coding (232/993 nt) wbbI beta‑1,6‑galactofuranosyltransferase
?NC_000913 2105842 = 10 (0.700)coding (216/993 nt) wbbI beta‑1,6‑galactofuranosyltransferase