Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F124 I0 R1
|
44 |
29.6 |
1159012 |
90.6% |
1050064 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,440,789 |
C→A |
24.9% |
intergenic (‑5/‑269) |
ydbK ← / → ydbJ |
putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,440,789 | 0 | C | A | 24.9%
| 61.6
/ 17.3
| 41 | intergenic (‑5/‑269) | ydbK/ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Reads supporting (aligned to +/- strand): ref base C (14/16); new base A (6/4); total (20/21) |
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 |
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAAC > NC_000913/1440657‑1440927
|
ccGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAACAGTCATCATATGACACCCTCACAt > 2:448080/1‑143 (MQ=255)
gcttCCGGCCCAGGCATCAGCCTGTTCTGTCATCGTGGAACTGGGGCTCCAAGTGGAGATCGAGATCTGTCTACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTg < 2:409372/141‑1 (MQ=255)
tttCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCCATAGTAATCATATGACACCCTTACATTgc > 2:568278/1‑143 (MQ=255)
tCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTCCATTccgc > 1:212488/1‑143 (MQ=255)
ccAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGa > 1:190748/1‑143 (MQ=255)
agccATCAGCCTGTTCTGCCATTGTGGAACTGGGGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAgg < 1:378057/140‑1 (MQ=255)
aGCCTGTTCTGCCAGTGGGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCAc < 2:320051/143‑1 (MQ=255)
ccTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGa < 1:347285/143‑1 (MQ=255)
gTTCTGCCATCGTGGAACTGGGGGTAAAAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAAt < 1:334204/143‑1 (MQ=255)
ttCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAAtt < 2:573266/143‑1 (MQ=255)
tGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATtgct > 1:575052/1‑143 (MQ=255)
gtCGTGGACCGGGGGGTGTTGGGGGGGATGTGGGTACCGTCACTGGTGCGAGATGCGACCGAAGCAACCGTGCCTTTACCGTCAATAGTGATCATATGACGTCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTgcgc < 2:489534/142‑1 (MQ=255)
aCTGGTGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagta < 1:80720/143‑1 (MQ=255)
cTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCagtag > 1:213139/1‑143 (MQ=255)
gggTAAGAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAAt < 2:520858/143‑1 (MQ=255)
tAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCa > 2:59110/1‑143 (MQ=255)
ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAGAGGCGGGGCGCACGAAATTGCTGCGCGCGCAGTGGTGATCTTTCAAtt > 1:35601/1‑143 (MQ=255)
aGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 2:198521/143‑1 (MQ=255)
aGATGGCGATAACTCCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 2:450613/143‑1 (MQ=255)
gCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCaaa < 1:59110/143‑1 (MQ=255)
ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCCGTAGTAATCTTTCAATTTTAGCAAATGGCTttc > 2:532497/1‑143 (MQ=255)
ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCCTTCAATTTTAGCAAATGGCTttc > 1:234652/1‑143 (MQ=255)
cgggTGCGATATGCGACCGAAGCCACCGCGCTACTCCCGTCAATAGGAGTCAGATGACACCCTTACATTCCGCCAATGAGGGGCGCACCAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATCTTAGCAAATGGCTTtcttct < 2:64779/141‑1 (MQ=255)
tGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTg < 1:133677/143‑1 (MQ=255)
ggTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGc < 1:401469/143‑1 (MQ=255)
aaaTGGCACCGCAGCAACCACGCCCTTACCGTCAATAGTAATCATATCACCCCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTc < 2:450505/143‑1 (MQ=255)
aaTGTGTCCGAAGCAACCGCGCCATTACCGTCAATAGGAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTGCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCg < 2:196369/143‑1 (MQ=255)
aCCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCGGCATTTTCGCTTTtg > 1:384232/1‑143 (MQ=255)
ccGAAGCAACCGCGCGATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGCGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTCCAATTTTAGCAAATGGCTATCTTCTGCATTTTCGCTTTtgt > 2:486382/1‑143 (MQ=255)
cAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCAAGAAATTGCTGCGCGCCCAGTAGTAATCTTTCTATTTTAGCAAATGACTTTCTTCTGCTTTTTGGCATTAGTTTcccc > 1:168899/1‑143 (MQ=255)
gcgcCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTCCATTTTCGCTTTTGTGTCCCCCACAt > 2:415134/1‑143 (MQ=255)
ccATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAg > 1:524495/1‑143 (MQ=255)
cAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACTAAATTGCTGTGCGCTCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaat < 1:393585/143‑1 (MQ=255)
cAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaatgaat < 1:69514/143‑1 (MQ=255)
tAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtt > 2:434516/1‑143 (MQ=255)
gTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt > 2:183098/1‑143 (MQ=255)
ctactCATATGATGCCCTTACACTGCGTCAATGAGGGCCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGtttt < 1:170410/139‑1 (MQ=255)
tCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCACCGTAATGATTGTTTTGCTc > 2:367828/1‑143 (MQ=255)
cATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCATCCAATTTTAGCAAATGGCTTTGCACTGCATGTACGCTTCTGTGTCCCCCACATCAGCGCAGTCAACGTTTTGACCa > 2:488756/1‑143 (MQ=255)
tatGAAGCCCTTACATTGCGCAAATGAGGCGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTCTACCAAATGGCTTTTTTCTTCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCaaa < 2:257624/143‑1 (MQ=255)
atGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGCAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATatttggtccacccgaaa > 1:219584‑M2/1‑126 (MQ=255)
|
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAAC > NC_000913/1440657‑1440927
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
