Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F124 I1 R1
|
28 |
40.1 |
1601150 |
89.4% |
1431428 |
142.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,440,789 |
C→A |
intergenic (‑5/‑269) |
ydbK ← / → ydbJ |
putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,440,789 | 0 | C | A | 88.9%
| 77.3
/ 1.1
| 37 | intergenic (‑5/‑269) | ydbK/ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base A (17/15); total (19/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 |
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACA > NC_000913/1440657‑1440928
|
ccGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACAt > 2:670811/1‑143 (MQ=255)
tttCCGGCACAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTgc < 1:466012/143‑1 (MQ=255)
ttCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTgcg < 2:262684/143‑1 (MQ=255)
cAGGCATCAGCCTGTTCTGCCATCGTGGAAATGGGGGTGGGATTGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTTCCGTCACTAGTAATCTTCTGAAACCCTTACATTGAGCAAATGAg > 1:152906/1‑143 (MQ=255)
cAGGCAGCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATCGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAAGCATATGAAACCCTTACATTGCGCAAATGAg < 2:211349/143‑1 (MQ=255)
tgtgCTGCCATCGTGGAACTGGGGGTCATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGaaa < 1:135180/143‑1 (MQ=255)
attcatGCCATCGTGGAACTGGTGCGAGTACGAAAGATTTCCTTACCTTCCCTGGTGGGAAATGCGACCGAAGCAACCGCGCTATTACCGTCAATAGTAATTATATGTACTTCTTTCATTGCGCTAATGAGGGGCGCACGaaa < 2:428030/138‑1 (MQ=255)
gtatagTGGAAATGGGGGTAATAGGGTAGATGGCGATAACTTCACAGGAGCGAAAAGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATtgctgc < 1:320610/138‑1 (MQ=255)
cATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATAGCTgcg > 1:13853/1‑143 (MQ=255)
cTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGGGCCATGCGACGGAAGCAACCGCGCCATCACCGTCAACAGTAATCATATGAAACCCTTACATTGCGCAAACGAGGGGCGCACGACATTGCTGCGCGCCcagcag > 2:651455/1‑143 (MQ=255)
ggTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTCCTCGGCGCCCAGAAGTaagc > 2:764923/1‑141 (MQ=255)
tAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCtt > 1:640680/1‑143 (MQ=255)
aGGGTAGATGGCGCTAACGTCACTGGTGCGAAATGCGACCGAGGCAACCGCGCCATTACCGTCAATAGTCATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCaa < 2:173999/143‑1 (MQ=255)
gggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGAGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGGACGAAATTGCTGGGTGGCTTGTAGTAATCGTTCAAt > 1:292221/1‑143 (MQ=255)
ggTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGCCACCCTTACATTGCGCACATGAGGGGCGCACGAAATTCCTGCGCGTCCGCTCGTAATCTTTCAAtt > 2:311393/1‑143 (MQ=255)
atttGGCGATACCGTCACTAGAGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTa < 1:275036/140‑1 (MQ=255)
ggCGATAACTTCACTGGTGCGAAATGCGACCGAAGCGAGCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAAGGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTCGC‑aa > 1:345795/1‑143 (MQ=255)
ctccACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACCTTGCTCAAATGAGGGCCGCACGAAAATCCTCCCCGCCCGCTCGTAACAGTTCAATCGTAGC‑AAATCGcgct > 2:46922/4‑140 (MQ=255)
cTTCACTGGTGCGAAGTGCGGACGAAGCCACCGCGGCATTAGCGTGAATGGTAAGGCGATGAGTCCCTTGCATTGCGGAAATGAGGGGCGCACGTTATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCttt < 2:748697/143‑1 (MQ=255)
ttCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTttc > 2:559574/1‑143 (MQ=255)
tGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTg > 1:156300/1‑143 (MQ=255)
gAAATGCGACTGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTGTGGC‑AAATGGCTTTCTTCTGCGtttt > 1:431825/1‑143 (MQ=255)
gAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGCAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAACCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCAtttt < 1:515023/143‑1 (MQ=255)
aaaTGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTc < 2:345190/143‑1 (MQ=255)
cGACCGAAGCAGCCGCGCCATTCCCGTCAATAGTAATCATATGAATCCCTTACATTGCGCAAATGAGGGTCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCttt < 2:151676/143‑1 (MQ=255)
gACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCtttt < 1:386066/143‑1 (MQ=255)
gAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGGAGTAAGCTTTCAATTTTACC‑AAATGGCTTCTTTCTGTTTTTTTGCTTTtgtga > 1:292589/1‑142 (MQ=255)
gAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCACAAGAAAGTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCACTTTTgtgt > 1:297763/1‑143 (MQ=255)
aaGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGCAAAATGGCTTTCTTCTGCATTTTCGCTTTTgtgt > 1:460567/1‑143 (MQ=255)
aaGCAACCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTc > 1:425424/1‑143 (MQ=255)
caCCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTccccc < 1:604906/142‑1 (MQ=255)
aCCGCGCCATTACCGTCAATAGTAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTcccccc > 1:193798/1‑142 (MQ=255)
ccGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCATTTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTaat < 2:396698/143‑1 (MQ=255)
tAATCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTg < 1:141305/143‑1 (MQ=255)
aaTCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGa < 1:283473/143‑1 (MQ=255)
aTCATATGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTGTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGAt < 1:335200/143‑1 (MQ=255)
tGAAACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATATTTCAATATTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAGTGTTTTGATCAAACa > 1:382640/1‑143 (MQ=255)
|
CCGTTTCCGGCCCAGGCATCAGCCTGTTCTGCCATCGTGGAACTGGGGGTAATAGGGTAGATGGCGATAACTTCACTGGTGCGAAATGCGACCGAAGCAACCGCGCCATTACCGTCAATAGTAATCATATGACACCCTTACATTGCGCAAATGAGGGGCGCACGAAATTGCTGCGCGCCCAGTAGTAATCTTTCAATTTTAGC‑AAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACA > NC_000913/1440657‑1440928
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
