breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 460,772 T→G S629A (TCG→GCG)  lon → Lon protease
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 802,778 G→T V298L (GTG→TTG)  ybhI → putative tricarboxylate transporter
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,440,789 C→A intergenic (‑5/‑269) ydbK ← / → ydbJ putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
RA 1,852,271 C→T R129C (CGG→TGC)  pncA → nicotinamidase
RA 1,852,273 G→C R129C (CGG→TGC pncA → nicotinamidase
RA 1,934,844 C→A E491* (GAG→TAG)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,145,777 G→A G838S (GGC→AGC)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,034,547 A→G E468G (GAG→GGG)  trkH → K(+) transporter TrkH
RA 4,183,275 C→G N677K (AAC→AAG rpoB → RNA polymerase subunit beta
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,204 G→A intergenic (‑105/+128) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696479 696575 97 15 [13] [12] 15 [glnX]–[glnV] [glnX],[glnV]
* * ÷ NC_000913 3423723–3424234 3424530–3424238 5–808 15 [14] [14] 17 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315852NA (NA)25 (0.600) 12/274 2.5 NA noncoding (624/1255 nt) IS3 repeat region
?NC_000913 = 315875 NA (NA)noncoding (647/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)2586 (61.010) 271/280 0.0 98.5% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 39 (0.910)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 57247139 (0.910)10 (0.310)
+34 bp
8/214 2.6 31.6% coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
?NC_000913 = 2170235 18 (0.420)noncoding (65/1258 nt) IS3 repeat region