Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F20 I1 R1	20	59.7	2309458	89.5%	2066964	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,111,164	G→T	R101L (CGT→CTT)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,111,164	0	G	T	94.2%	133.8 / ‑4.2	54	R101L (CGT→CTT)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand): ref base G (2/0); major base T (22/27); minor base C (1/2); total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAC  >  NC_000913/1111026‑1111296

tGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTc                                                                                                                                  >  1:957226/1‑143 (MQ=255)

  ggaCAAGCCTGGCCAGGTTGACTGGCTGATGGAGAGTTGATTAAAGGCGGGGAGGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg                                                                                                                                <  1:1089895/141‑1 (MQ=255)

  gatcAAGCCTGGTCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg                                                                                                                                <  1:981477/140‑1 (MQ=255)

  gAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCACCTGGGTAGACCTGTTCTg                                                                                                                                >  1:151075/1‑143 (MQ=255)

  gAACAAGCCTGGCCAGATTCACGTGCTGATGGACAGTTAATTGAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCCCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTg                                                                                                                                <  1:1149630/143‑1 (MQ=255)

   aaCAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCTATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTgg                                                                                                                               >  2:706184/1‑143 (MQ=255)

     cAAGCCTGGCCAGCTTCCCTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGACGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGa                                                                                                                             <  2:869527/143‑1 (MQ=255)

         ccTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCACGATGTTTCCCGAACCGTG‑GCGTACCACCCCGGTAGGGCGTTGCTGGGATCGc                                                                                                                         >  2:323170/1‑143 (MQ=255)

             gCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGc                                                                                                                     >  1:562255/1‑143 (MQ=255)

             gCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACAAACCCGGTAGGCCTTTGCTGGGCTCGCCTGc                                                                                                                     >  2:825333/1‑143 (MQ=255)

               taGATTCCCGTGCTTTTGTTCAGTTATTTAAAGACGACGAATGGCGCGATCAGCTGCAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCTCGACTCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt                                                                                                                   <  2:1028/142‑1 (MQ=255)

               cAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt                                                                                                                   <  1:221120/143‑1 (MQ=255)

               cAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGt                                                                                                                   <  1:467523/143‑1 (MQ=255)

                  aTTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCCGTAGACACTTTCTTGGGTGGGCTGCGTgca                                                                                                                >  2:743856/1‑141 (MQ=255)

                      aCTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTCCGGACCCGGTAGACGTTTTCTGGGATCGCCTGCGTGGAcgcg                                                                                                            >  2:503155/1‑143 (MQ=255)

                            tGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCACG‑CTATTGATCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCa                                                                                                      >  1:1143016/1‑143 (MQ=255)

                              aTGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcg                                                                                                    <  1:911937/143‑1 (MQ=255)

                               tGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCTACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCAcgc                                                                                                   >  1:351092/1‑143 (MQ=255)

                                   cAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCAGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCcgcg                                                                                               <  1:1032360/143‑1 (MQ=255)

                                          ttaaAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTg                                                                                        >  2:96274/1‑143 (MQ=255)

                                                   acgaAGGAGGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTg                                                                               <  1:344038/143‑1 (MQ=255)

                                                    cgaAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGa                                                                              <  1:96274/143‑1 (MQ=255)

                                                        gtgcgcTATCAGCTGGAGGCGATGCCAGAACCACAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCACCCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCaa                                                                          <  2:495964/141‑1 (MQ=255)

                                                         ggCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGAGCAGACGTCACGCTTCGCTATCTGGCTCGTTTGACCaca                                                                         >  1:1084677/1‑141 (MQ=255)

                                                         ggCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCaaa                                                                         >  1:694119/1‑143 (MQ=255)

                                                              gATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAagaaga                                                                    <  2:268638/143‑1 (MQ=255)

                                                               atcatcTGTAGGCGACGCTAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGTTAGGCCTGTCCTGAGATCGCCTGCGTGTACGCGATGTCACGCAGCGCTATCTGGCTCGTTTGACCAAATAagag                                                                   <  2:983115/143‑1 (MQ=255)

                                                                  aGCTGAGGGCGATGGCAGAAGCAAAACGCTCCGCGATGTTTCCCGACCCGTG‑GCGGACCTACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAg                                                                <  1:34271/143‑1 (MQ=255)

                                                                  aGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCCCGCCCCGCTATCTGGCTCGTTTGACCACAGAACGGCAg                                                                >  2:149502/1‑143 (MQ=255)

                                                                  aGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTCTCTGGCTCGTTGGACCAAAGACGAGCAg                                                                >  1:650940/1‑143 (MQ=255)

                                                                        aGGCGATGCCAGCAGCAAAACGCTCCTCGATGTTTGCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGGCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagc                                                          <  2:493312/143‑1 (MQ=255)

                                                                         ggCGATGCCAGAAGCAACACGCTCCTGGGTGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGAGCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcg                                                         <  2:327789/143‑1 (MQ=255)

                                                                           cGAGGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcgag                                                       <  1:520736/143‑1 (MQ=255)

                                                                            gATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCCATCTTGCTCGTTTCGACAAAGAAGCGCAGGAaagcgagc                                                      >  2:288734/1‑143 (MQ=255)

                                                                            gATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCCTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAgagcgagc                                                      <  1:581546/143‑1 (MQ=255)

                                                                                ccAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCaaaa                                                  <  2:351092/143‑1 (MQ=255)

                                                                                  aGAAGCAAACCGCTCCCCGCTGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGTACGCTATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGt                                                <  1:70488/143‑1 (MQ=255)

                                                                                   gAAGCAAAACGCTCCTCGATGTTTCCCAACCCGCA‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGtt                                               >  1:723042/1‑142 (MQ=255)

                                                                                         aatcGCTCCTCGTTGTTTTCTGTATTGTG‑TCGACCCAACCCGGTAGGCCTTTGCGGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTAc                                         <  1:777128/140‑1 (MQ=255)

                                                                                               tcctcGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAACGGCGAGCAGGCGAGCGAGCAAAAGTGGCGTACCGTCgg                                   >  1:268447/1‑143 (MQ=255)

                                                                                                 ctcGTTGTTTCCCGGGCCGGG‑GCGTCCCGACCCGGTAGGCCTTTTCTGGGGTCGCCTGCGTGGACGCGAGGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTa                                 <  2:933616/143‑1 (MQ=255)

                                                                                                      tGTTTCCCGACCCGGG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGGCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATc                            <  2:399557/143‑1 (MQ=255)

                                                                                                        tctcacGACGCGCG‑CCGTGCCACTCCGGTACGCCCGTTATGGGTTATCCTTCGTAGACGCGATGTCACGCCGCGCTATCTGTCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccg                          <  2:749942/138‑1 (MQ=255)

                                                                                                          tCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccgcc                        >  1:976108/1‑143 (MQ=255)

                                                                                                          tCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCGGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATccgcc                        <  1:687496/143‑1 (MQ=255)

                                                                                                            ccGACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTGTCTGGCTCGTTTGACCCGAGAAGAGCAGGAGAGGGAGCGAAAGTGGGGTGCCGTCGGTACCAGccgccgc                      >  2:130222/1‑142 (MQ=255)

                                                                                                              gACCCGTG‑GCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGGTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTa                    >  2:418276/1‑143 (MQ=255)

                                                                                                                   ggg‑gCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGAGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTc               <  1:212638/141‑1 (MQ=255)

                                                                                                                       gCGTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGt            >  1:1071743/1‑143 (MQ=255)

                                                                                                                         gTACCAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTg          <  1:107417/143‑1 (MQ=255)

                                                                                                                            ccAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGGTGCATTCTGTTGCTc       >  1:144321/1‑143 (MQ=255)

                                                                                                                             cAACCCGGTAGGCCTTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATcc      >  1:361682/1‑143 (MQ=255)

                                                                                                                              aaCCCGGTAGGCCGTTTCTGGGATAGCCTGCGTGGACGCGATGTCACGCGGCGCTACCTGGGTCGTTTGCCCGAGGAAGAGCAGGAGAGCCAGCAAAAGGGACATACCGACCGTACCAGCGCCCGCTGCAGTATATTGATCCt     >  2:777399/1‑143 (MQ=255)

                                                                                                                                 gcGGGAGGCCTTTGCGGGGCTCGCCTGCGTGGGCGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAc  <  2:752948/142‑1 (MQ=255)

TGGAACAAGCCTGGCCAGATTCACTTGCTGATGGACAGTTAATTAAAGACGACGAAGGGCGCGATCAGCTGAAGGCGATGCCAGAAGCAAAACGCTCCTCGATGTTTCCCGACCCGTG‑GCGTACCAACCCGGTAGGCCGTTTCTGGGATCGCCTGCGTGGACGCGATGTCACGCCGCGCTATCTGGCTCGTTTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGAC  >  NC_000913/1111026‑1111296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A