Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F59 I0 R1
|
60 |
41.1 |
1578944 |
90.5% |
1428944 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
449,135 |
C→A |
100% |
V503L (GTA→TTA) |
cyoB ← |
cytochrome bo3 ubiquinol oxidase subunit 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 449,135 | 0 | C | A | 100.0%
| 82.6
/ NA
| 30 | V503L (GTA→TTA) | cyoB | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/18); total (12/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAAC > NC_000913/448998‑449268
|
cGGAGGCGGGGAAGAGGTGGCCCACTCCAGCGGACGGCCACCCCCCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCAc < 1:293069/143‑1 (MQ=255)
gAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGc > 1:212628/1‑143 (MQ=255)
ccctcaggCGTGCGGCCCCCCCACGGCTCGCCAGTCGGGTCAGGGTTTTGTGCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCAt < 2:377741/136‑1 (MQ=255)
tCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAGACGTACATCTGAATAACGAGGCCGAGAATACCCAGCGCCATCCGTAATGCACCGCTGCCCGCAAACCACAGCacgct > 2:71057/1‑139 (MQ=255)
cAGCGTACGGCCACCCCCCTGGGCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGgtgt < 2:241921/143‑1 (MQ=255)
aGCGTGCCGCCCCCCCACGGGGCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGtgtg < 2:564395/143‑1 (MQ=255)
ggCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCCTGGTGTGGAACTGc > 1:645795/1‑143 (MQ=255)
ggCCACCCCACGGGTCGCCAGTCAGATCACGGTTATGGTCGCGGTCGCGAATAGAAACGTACATCGGACTAACGAGGCAGAGGATAACCAGCGCAATAAGTAATGCACCGCTGGCTGCAATCAACAGCATGGGATGGAACAGc > 2:28877/1‑143 (MQ=255)
cccccccTCGGGTCGCCAGGCAGGTCAGGGTTCTGGGCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAAGCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCgg < 2:180423/140‑1 (MQ=255)
gTCGCCAGGCCGGCCACGGGTCTGGTCGCGGTCGCGAATAGAACCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATctgct < 2:481908/143‑1 (MQ=255)
gcGCCAGTCAGGTCCCGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATctgctg < 2:81465/142‑1 (MQ=255)
gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCaa < 2:83978/134‑1 (MQ=255)
gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCaa > 1:83978/1‑134 (MQ=255)
cAGTCAGGTCACGGTTCCGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTc < 1:252169/143‑1 (MQ=255)
cACGGTTCTGGTCGCGGTGCCCACTAGGAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACgg < 2:703955/143‑1 (MQ=255)
gggcgcgAAGAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCTCCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAg < 2:633154/140‑1 (MQ=255)
gcgAAGAGAGACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa < 2:435737/143‑1 (MQ=255)
aaaCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATAc > 2:448650/1‑143 (MQ=255)
gcGTACACCTGAGCACCGAGGCAGGGAACACCCGGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCTTACAg < 2:393029/142‑1 (MQ=255)
aCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGACGCCCAGCGCATACAg > 1:275765/1‑143 (MQ=255)
ccaCATCTCAATAACGGGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTg < 1:41217/141‑1 (MQ=255)
gAATAACGAGGGAGAGAATACCCCGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAg < 1:286062/143‑1 (MQ=255)
aaTAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAgg > 1:283405/1‑143 (MQ=255)
aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGACGCCCAGCGCATACAGAGGCAGCAAGGGaa > 2:483447/1‑143 (MQ=255)
aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCaa < 2:652202/143‑1 (MQ=255)
aaCGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCaa < 2:212628/143‑1 (MQ=255)
gCAGAGAATACCCAGCGCACTCAGTAATGCACCGCTGGCTGCAACCATCAGCATCGTGTAGAACTGCGGCTCAATCTGCTGGCTCAGACGGCGGGCCATGCCCATGAAGCCCAGCGCACACGGTGGCATAAAGGcgacgacga > 2:402868/1‑143 (MQ=255)
gCAGAGAATACCCAGCGCAATCAGCAATGCACCGCAGGCTGCAAGCCTCAGCATGGGGTGGAACTGCGGGGCAGACCGTCGGCTCGGCCGACGGGGCGAGCCCATGAGGCCCGGCCGACGCAGTGGCAGAGAGGCagagacgg > 2:634248/1‑136 (MQ=255)
aGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATcca > 1:199666/1‑143 (MQ=255)
cacccAGTAATGCACCGCTGTCTGCAATCATCACCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGCCGGGTCACGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaac < 1:200380/139‑1 (MQ=255)
|
CGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAAC > NC_000913/448998‑449268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
