Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F97 I0 R1
|
51 |
42.6 |
1617048 |
90.7% |
1466662 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
449,135 |
C→A |
100% |
V503L (GTA→TTA) |
cyoB ← |
cytochrome bo3 ubiquinol oxidase subunit 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 449,135 | 0 | C | A | 100.0%
| 59.3
/ ‑6.7
| 23 | V503L (GTA→TTA) | cyoB | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (17/5); minor base G (0/1); total (17/6) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAA > NC_000913/448996‑449273
|
aaCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGCCGCCAGTCAGGTCACGATTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGc > 1:629628/1‑143 (MQ=255)
cGGGGAAGAGGTTGCACACTCCAGCGTACGGCCACCCCACGGGTCGCCAGGCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCgctgg < 2:793250/143‑1 (MQ=255)
ggTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAatca > 2:60043/1‑143 (MQ=255)
ttGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCCGCAatcatc > 1:806382/1‑143 (MQ=255)
ggCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGc > 1:758003/1‑143 (MQ=255)
ccACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCCTGGTGTGGAACTGCgg > 2:531575/1‑143 (MQ=255)
aCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACTCAGCGAAATCAGTAATGGACAACTGGCTGAAAACATCAGAATGGTGTGGAACTGCGAGTCAATc > 1:24192/1‑143 (MQ=255)
gCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATATACATAACGACCCGTAGAATCCTGCGCCCAACCAGTAATGCACCGCAGGCTGCAATCATCAGCATGGTGTGGACCTGCGGCTCAATCTGCTGGc > 1:373744/1‑143 (MQ=255)
cACGGTGCTCGTCGCCGTCGCGAATAGAAGCGTACATCTGAATAACGAGGCAGAGACTACCCAGCGCAGTCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGGGGAACTGCGGGTCAATCTGCTGGCTCAAACGACgg < 2:793650/143‑1 (MQ=255)
tcgcgAATAGAAGCGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGcc > 1:161259/1‑143 (MQ=255)
gcgAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa < 1:691578/143‑1 (MQ=255)
gcgAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCa > 1:705285/1‑143 (MQ=255)
tACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGAGAAAAATTATGAATTTGTCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTgg > 1:184519/1‑143 (MQ=255)
aTCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCATTCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCAt > 1:754284/1‑143 (MQ=255)
cTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATaa > 1:530182/1‑143 (MQ=255)
gAGGCAGAGAATACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGCGGAACTACGGGTCAATCTGCTGGCTCAAACGACGCGTCACGCCCATGACACCCAGCACACACAATCACAAAAAAGCAAccc > 2:671703/1‑141 (MQ=255)
cAGAGAACACCCAGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACgaagaa < 1:426370/143‑1 (MQ=255)
gaATACCCAGCGCAATCCGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCg < 1:60043/143‑1 (MQ=255)
cccAGCGCAATCAGTAATGCACCGCTGGCTGCAATAATCAGCATGGTGGGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATGAAGGCAACGAAGAAGGCgatgat > 1:33975/1‑143 (MQ=255)
aGCGCAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCAAGCGGAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCAGATGATgca > 1:113692/1‑143 (MQ=255)
gcgcAATCAGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCCACGAAGCAGCAGATGATccag > 2:448258/1‑143 (MQ=255)
agtCAGTAGGGCCCCGCTGGCTGCGATCATCAGCATGGTGTGGGACTGCGGGTCAATCGGCGGGCTCAAACGGCGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaacc < 2:654718/141‑1 (MQ=255)
aGTAATGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACCGCGGGTCAATCTGCTGGCTCAAACGACGGGGCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAGGCCGCTGATccggaacccgcc > 1:250744/1‑139 (MQ=255)
|
AACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAA > NC_000913/448996‑449273
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
