Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F83 I0 R1	128	58.1	2217184	91.0%	2017637	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,196,204	G→A	74.5%	intergenic (‑105/+128)	thiC ← / ← rsd	phosphomethylpyrimidine synthase/regulator of sigma D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,204	0	G	A	74.5%	85.8 / 29.3	62	intergenic (‑105/+128)	thiC/rsd	phosphomethylpyrimidine synthase/regulator of sigma D
Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (22/22); total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

TTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTT  >  NC_000913/4196067‑4196346

ttgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGGTGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGt                                                                                                                                             >  1:85070/1‑143 (MQ=255)

   ttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTAtt                                                                                                                                          >  2:639665/1‑143 (MQ=255)

      gcggcgGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGc                                                                                                                                       <  1:642718/143‑1 (MQ=255)

          cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGa                                                                                                                                   >  1:26756/1‑143 (MQ=255)

          cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGa                                                                                                                                   >  1:368185/1‑143 (MQ=255)

           gggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGAt                                                                                                                                  <  1:910080/143‑1 (MQ=255)

                aGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTGGCCTGAGCAGGt                                                                                                                             >  1:146699/1‑143 (MQ=255)

                  ttttGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAACCCACAGAAGAAGCAAGAGACGAGCGCCGCAGGGGCGATGCGATAGCAGATTAACCCTTGTTCCCTTCACAGGTATTAGCCGGATCAGGGTc                                                                                                                           >  2:786293/1‑143 (MQ=255)

                       ttgCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCgg                                                                                                                      >  2:565215/1‑143 (MQ=255)

                                  gCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATaa                                                                                                           >  1:700155/1‑143 (MQ=255)

                                   cTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATaaa                                                                                                          >  1:1105750/1‑143 (MQ=255)

                                    tCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCACTTCGCAGGTATTAGCCTGATCAGGTTCCGCCGATCCCGAATaacc                                                                                                         >  2:343537/1‑141 (MQ=255)

                                     cATTCCAAAAGGTTACGGACGTGGCTTGTCAGCCGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTGGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACg                                                                                                        <  2:905073/143‑1 (MQ=255)

                                        tCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGtc                                                                                                     <  1:166364/143‑1 (MQ=255)

                                          cAAAAAGTTACGGACGTGGCTTGTCAGACGACGGCTGAAGCAAGAGACGCTCGCCGCAGGGGCGCTGCGGTCGCGGCTTAACTCTTGTTCCCTCGCCGGGCGTTAGCCTGCTCAGGTGCCGCGGAGCCCGCATCCACgggcgc                                                                                                   >  1:40065/1‑139 (MQ=255)

                                          cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGTGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGtct‑c                                                                                                  <  1:98264/143‑1 (MQ=255)

                                          cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGtct‑c                                                                                                  >  1:499061/1‑143 (MQ=255)

                                          cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGtct‑c                                                                                                  >  1:531171/1‑143 (MQ=255)

                                          cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGtct‑c                                                                                                  >  1:125294/1‑143 (MQ=255)

                                           aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑Ca                                                                                                 <  2:1050949/143‑1 (MQ=255)

                                           aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGAGTAACTCTTGTTCCCTGCACAGGTGTTAGCCTGAACAGGTCCCGCGGATCCCGAGTAAACGCTCT‑Ca                                                                                                 >  2:69800/1‑143 (MQ=255)

                                             caaGTTAAGGGCGTTGCTTGTCAGACTACGGACACAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTATCTCTTTTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGc                                                                                               <  1:398346/142‑1 (MQ=255)

                                                     ggACGTGGCTTGGCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATGAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAg                                                                                       <  2:168648/143‑1 (MQ=255)

                                                        cGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCa                                                                                    <  1:306597/143‑1 (MQ=255)

                                                           ggCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTc                                                                                 >  1:961988/1‑143 (MQ=255)

                                                             cTTCTCACACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCg                                                                               <  1:114053/143‑1 (MQ=255)

                                                              ttGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGa                                                                              <  1:639665/143‑1 (MQ=255)

                                                                gTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGCCT‑CAGCCCGTCAAGGCCCCCCGAcc                                                                            >  1:951942/1‑142 (MQ=255)

                                                                 tCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACaa                                                                           <  1:178529/143‑1 (MQ=255)

                                                                   aGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGACTAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACACGa                                                                         >  2:917726/1‑143 (MQ=255)

                                                                      cgacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAAt                                                                      <  2:368185/143‑1 (MQ=255)

                                                                       gacgGATGAAGCTAGAGACGTTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCGGATCAGGTGCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAAtt                                                                     <  1:129734/143‑1 (MQ=255)

                                                                        acgGATGCAGCAAGAGACGCTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTa                                                                    <  2:744246/143‑1 (MQ=255)

                                                                            aTGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGccc                                                                >  2:686944/1‑143 (MQ=255)

                                                                             tGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCACTTCACAGGTCTTAGCCTCATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCGCTCCGACAGGAACTGAGcccc                                                               >  1:210804/1‑143 (MQ=255)

                                                                                 gCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑aa                                                          >  1:900622/1‑143 (MQ=255)

                                                                                      agaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATggg                                                     <  2:26756/143‑1 (MQ=255)

                                                                                      agaCGACCGCCGCAGGGGCGATGCGATAGCAGATTAACTCGTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTGAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATggg                                                     <  2:740275/143‑1 (MQ=255)

                                                                                       tcgcaTCGCCCCTGCGGCGATGCGATAGCAGATTAACTATTGTTCCCTTCACCGGTATTAGCCTGATCACGTGCCGCGGATCGCGAATAAACGGTCTCCGGCCAG‑TAAGGCACTCCGACAAGAAATTAGCCCCAC‑CAAGgggg                                                    >  2:513003/5‑143 (MQ=255)

                                                                                       gacgacCCCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATgggg                                                    <  1:701745/143‑1 (MQ=255)

                                                                                           aTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTCTTAGCCTGATCAGGTTCCGCGGCTCCCGAATCCACGGTCT‑CAGCCCGTTAAGGCACTCAGACAAGAAAATAACCCCGC‑AAACGGGACacg                                                >  1:126716/1‑141 (MQ=255)

                                                                                           aTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACGGGTATTAGCCTGAGCAGGGTGCGCGGATCCCGAATAAACGGTCT‑CAGCCGGTTAAGGAGCTCCGGAGAGAAGTTGGCCCCGC‑AGAGGGGGCAtt                                                >  1:95876/1‑143 (MQ=255)

                                                                                           aTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCACTACCCAGCTTATTGACTGATGAGGTTTATAGTTTCCCTATGAAACGGTCT‑CAGCCCGTTACGGCACTCCGACAAGAAATTAGCCCCGC‑AGATGGGGCAtg                                                >  1:257478/1‑142 (MQ=255)

                                                                                               ccgcTGGCGCGATGCGCTCGCATACTAGCTCTTGTTCCCGCCACAGGTATTCGCCTGATCAGCTTCCGCGGATCCCGAATACACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAAt                                            <  2:576786/143‑1 (MQ=255)

                                                                                               ccgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCAAGCCACACCACTCCGACAAGAACGGAGCCCCGC‑AAATGGGGCATTGagt                                            >  2:658028/1‑141 (MQ=255)

                                                                                               ccgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTAATAGCCTGATCAGGTTCCGCGGAGCCCGAATAAACGGTGT‑CAGCCAGTTAAGGCACTGCGACAAGAAATTAGGCGCGC‑AAATGGGGCAtgggat                                            >  1:227502/1‑138 (MQ=255)

                                                                                                cgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATg                                           >  2:201887/1‑143 (MQ=255)

                                                                                                    ggggCGATGCGATAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATGCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAAATGGGGCCTTGAATGtca                                        >  1:155765/1‑141 (MQ=255)

                                                                                                       gcgatgcgatAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTa                                    <  1:966950/143‑1 (MQ=255)

                                                                                                         gatgcgatAGCAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGACAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTAcg                                  >  2:297798/1‑143 (MQ=255)

                                                                                                                   cAGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAgc                        >  2:357243/1‑143 (MQ=255)

                                                                                                                    aGATTAACTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAgcg                       <  2:125294/143‑1 (MQ=255)

                                                                                                                    aGATTAACTCTGGTTCCCTTCACAGGCATTAGGCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGGCAAGAAATTAGCCCGGC‑ACGTCGGTCACTGAATGTAAATTACGCGTTAACAgcg                       <  2:907176/143‑1 (MQ=255)

                                                                                                                         aaCTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGaa                  <  2:700155/143‑1 (MQ=255)

                                                                                                                         aaCTCTTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAGACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGaa                  <  1:641454/143‑1 (MQ=255)

                                                                                                                            tctTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCCTTGAAAGTAAAGTACGCGTTAACAGCGCAGAACTc               >  1:623780/1‑143 (MQ=255)

                                                                                                                             ctTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCa              <  2:85070/143‑1 (MQ=255)

                                                                                                                             ctTGTTCCCTTCACAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCa              <  1:651859/143‑1 (MQ=255)

                                                                                                                              tcgTTCCCTTCGCAGGTATTCGCCTTATCAGGTTTCGCGGATCCCTAATCAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGATATTAGCCCCTC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCaa             <  1:321084/141‑1 (MQ=255)

                                                                                                                                ggtCCCTTCGCAGGCATGCGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGc           <  1:293057/141‑1 (MQ=255)

                                                                                                                                    ccTTCACAGGTAGTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACGCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGa       <  2:40242/143‑1 (MQ=255)

                                                                                                                                      ttCGCTGGTGTTTGCCTGGTCATGTTCCGCGGATCCCGATTAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTATATTACGCGTTAACAGTGCAGAACTCAAGCAGGATg     <  2:402893/143‑1 (MQ=255)

                                                                                                                                         gCAGGTATTAGCCTGATGAGGTTCCGCGGATCCCGAATAAGCGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGttt  <  1:300816/143‑1 (MQ=255)

                                                                                                                                         gCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATACACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGttt  <  2:14232/143‑1 (MQ=255)

                                                                                                                                         gCAGGTACTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGttt  <  1:144/143‑1 (MQ=255)

TTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCT‑CAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGC‑AAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTT  >  NC_000913/4196067‑4196346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A