Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F107 I1 R1	26	57.3	2246500	90.0%	2021850	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,196,219	A→C	intergenic (‑120/+113)	thiC ← / ← rsd	phosphomethylpyrimidine synthase/regulator of sigma D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,219	0	A	C	98.6%	227.3 / NA	72	intergenic (‑120/+113)	thiC/rsd	phosphomethylpyrimidine synthase/regulator of sigma D
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (37/34); minor base G (0/1); total (37/35)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGC  >  NC_000913/4196080‑4196358

gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCa                                                                                                                                          <  2:26348/143‑1 (MQ=255)

gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCa                                                                                                                                          <  1:77369/143‑1 (MQ=255)

  cAGTTTTGTTGCAGACACAGCTCATCCCAAAAAGTTAAGGCCGTGGCTTGTCAGACGACGGATGCAGCAAGTGATTTTCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAgg                                                                                                                                        <  1:209909/143‑1 (MQ=255)

   aGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAgtg                                                                                                                                       >  2:957966/1‑141 (MQ=255)

             cctaCATAGATCATGCCAAGTAGTTATGGACGTGGGTTGCCAGACGACGGATGAAGCAAGAGACGATCGCCGCCGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATc                                                                                                                             <  2:783358/140‑1 (MQ=255)

                aCATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCg                                                                                                                          >  1:113298/1‑143 (MQ=255)

                aCATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTAAGGTTCCGCGGATCCCg                                                                                                                          <  2:513905/143‑1 (MQ=255)

                aCATACCTCACTCCAAAAAGTTAACGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCCCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCg                                                                                                                          <  1:289939/143‑1 (MQ=255)

                  aTAGCTCATTCCAAAATGTTAAGGCCGTGGCTTGGCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGTTAGCAGATGAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGaa                                                                                                                        <  2:1087343/143‑1 (MQ=255)

                  aTAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGATGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGaa                                                                                                                        <  1:852589/143‑1 (MQ=255)

                   tAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAAt                                                                                                                       >  1:633199/1‑143 (MQ=255)

                    aGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCCGCCGCCCGACGAAGCAAAAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATGAGCCTGCTCGGGTTCCGCGGATccccacta                                                                                                                      >  2:612534/1‑138 (MQ=255)

                    aGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATAGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATa                                                                                                                      <  1:570688/143‑1 (MQ=255)

                      ctaaTTCCAAGAAGGTAAGGACGGGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGGTCAGGTTCCGCGGATCCCGAATaaa                                                                                                                    <  2:519369/140‑1 (MQ=255)

                        cATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACg                                                                                                                  <  1:497079/143‑1 (MQ=255)

                             cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATCGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGGTCCCGAAGAAACGGtctc                                                                                                             >  1:426174/1‑143 (MQ=255)

                             cAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTCCTCAGGTTCCCCGCACCCCGCATCAACCGtccc                                                                                                             >  1:936082/1‑141 (MQ=255)

                              aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTGGCAGGTATTGGCCTGCTCGGGTTCCGCGGATCACGAATAAACGGGCTCa                                                                                                            >  1:926463/1‑143 (MQ=255)

                              aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATGCCGAATAAAAGGTCTCa                                                                                                            >  2:70600/1‑143 (MQ=255)

                              aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCa                                                                                                            >  2:130746/1‑143 (MQ=255)

                              aaaaaGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGAAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCa                                                                                                            >  1:235985/1‑143 (MQ=255)

                                      aaGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCCCAGCCAGTTa                                                                                                    >  2:157052/1‑143 (MQ=255)

                                       aGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTaa                                                                                                   >  1:69376/1‑143 (MQ=255)

                                         ggcGTGGCGTGTCAGGCGAGGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg                                                                                                 <  2:936082/141‑1 (MQ=255)

                                         gACGTGGCTTGTCAGACGACGAATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATGAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTAAGGTTACGCGGATACCGAATAAACGGTCTGAGCCAGTGAAgg                                                                                                 >  2:310586/1‑143 (MQ=255)

                                         gACGTGGCGAGTCAGACGACGGATGACGCAACACCAACTCTTTGTACGGCGGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAgg                                                                                                 <  1:810414/143‑1 (MQ=255)

                                           cGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCa                                                                                               <  2:341858/143‑1 (MQ=255)

                                              ggCTTGTGAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTc                                                                                            <  2:953726/143‑1 (MQ=255)

                                              ggCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTc                                                                                            <  2:955679/143‑1 (MQ=255)

                                               gCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCGGCGGATCCCGAATAAACGGTCTCAGCCCGGTAAGGCACTcc                                                                                           >  1:633379/1‑143 (MQ=255)

                                               gCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTCAGGCACTca                                                                                           >  1:335014/1‑142 (MQ=255)

                                               gCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTcc                                                                                           >  1:522536/1‑143 (MQ=255)

                                                 ttGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAGCGGTCTCAGCCAGTTAAGGCACCCCgc                                                                                         >  2:421506/1‑142 (MQ=255)

                                                  tGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGAc                                                                                        <  2:69376/143‑1 (MQ=255)

                                                   gTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCCCAGCCAGTTAACGCACCCCGACa                                                                                       >  2:1078515/1‑143 (MQ=255)

                                                      aGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGa                                                                                    >  1:1008932/1‑143 (MQ=255)

                                                        acgacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGtt                                                                                                     <  1:95543/124‑1 (MQ=255)

                                                        acgacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGtt                                                                                                     >  2:95543/1‑124 (MQ=255)

                                                         cgacgGATGGCGCAAGTGCCGCTCGCCGCAGGTGCGATGCGATAGCAGATTAACTCTTGTTCCCCTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAAt                                                                                 <  2:896908/143‑1 (MQ=255)

                                                          gacgGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAAtt                                                                                >  1:961099/1‑143 (MQ=255)

                                                            gggATGAAGCAATAGACGATCGTCGATAGGGCGATGCGCTGGCAGATTGTCTGTTGTTCCCTTCGCAGGTATTAGCCGGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAg                                                                              <  2:664129/142‑1 (MQ=255)

                                                                   aGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTTAATATTGATTTATCCCCATTTGTTGGCCCCCACAGACTCCGCGGATCCCGAGTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCa                                                                       <  2:1090715/143‑1 (MQ=255)

                                                                   aGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGGATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACCAGAAATTCGCCCCGcc                                                                       >  2:152406/1‑142 (MQ=255)

                                                                       agagACGATCGCCGCTGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTACTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATg                                                                   <  2:952906/143‑1 (MQ=255)

                                                                       agagACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCATGCTCAGGTTCCGCGGATCCCGACTAACCGGCCTCCGCCAGCTAACGCCCTCCGACCAGAACTTCGCCACGCACATg                                                                   >  1:391247/1‑143 (MQ=255)

                                                                        gagaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAACTgg                                                                  >  1:951522/1‑143 (MQ=255)

                                                                        gagaCGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTGCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATgg                                                                  <  2:599756/143‑1 (MQ=255)

                                                                             gATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTGAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCCCTCCGACACGAAATTAGCCCCGCAAATGGGGCAt                                                             >  2:1041665/1‑143 (MQ=255)

                                                                             gATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCAt                                                             <  1:1089074/143‑1 (MQ=255)

                                                                                   cgcAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATg                                                       >  1:168227/1‑143 (MQ=255)

                                                                                            gatgcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcg                                              <  1:465519/143‑1 (MQ=255)

                                                                                             atgcgatAGCAGCTTAACTCTTGTCCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTAcgc                                             <  2:931366/143‑1 (MQ=255)

                                                                                               gcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGt                                           <  1:200219/143‑1 (MQ=255)

                                                                                               gcgatAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGt                                           <  1:200245/143‑1 (MQ=255)

                                                                                                   tAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCACATGGGGCATTGAATGTAAATTACGCGTTAAc                                       >  1:41069/1‑143 (MQ=255)

                                                                                                    aGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAAGAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACa                                      <  2:28626/143‑1 (MQ=255)

                                                                                                      cAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAgc                                    >  1:672089/1‑143 (MQ=255)

                                                                                                      cAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATGAGCCCCGCAAATGGGGCAGTGAATGTAAATTACGCGTTAACAgc                                    >  2:596486/1‑143 (MQ=255)

                                                                                                      cAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGACCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAgc                                    <  2:809049/143‑1 (MQ=255)

                                                                                                            acctgttGCTCCCTCCGCAGGTATTAGCGTGCTGAGGTTCGGGGGGTCCAGAATAAACGGTCTCAGCGAGTTAAGGCACTCGGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGaa                              <  1:386655/138‑1 (MQ=255)

                                                                                                            aaCTCTTGTTCCCTTCGCAGGCATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAACTGGGGCGTCGTATGTAAATTACGCGTTAACAGCGCAGaa                              >  2:228944/1‑143 (MQ=255)

                                                                                                              ctctTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGAGCATTGAATGTAAATTACGCATTAACAGAGCAGAACt                            >  1:280042/1‑143 (MQ=255)

                                                                                                               tctTGTTCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTc                           >  1:1114255/1‑143 (MQ=255)

                                                                                                                    ttCCCTTCGCAGGTCTTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCCAATGGGGCATTGAGTGTAAATTACGCGTTCACCACGCAGAAGTCAAGCa                      >  1:1071288/1‑143 (MQ=255)

                                                                                                                    ttCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGAGAAGAAATTAGCCCCGCAACTGGGGCATGGAATGTAAATTACGGGTTAACAGCGCAGAACTCAAGCa                      >  1:346939/1‑143 (MQ=255)

                                                                                                                     tCCCTTCGCATGTATGAGCCTGCTCAGGTTCCGCGGAGCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAAGTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAg                     <  1:200335/143‑1 (MQ=255)

                                                                                                                     tCCCTTCGCAGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAg                     >  2:143900/1‑143 (MQ=255)

                                                                                                                      cccTTCGCAGGTATTAGCCTGCTCAGGTGCCGCGGATCCCGAATAAACGGGCTCAGTCAGTTAAGGCACCCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAgg                    <  1:745018/143‑1 (MQ=255)

                                                                                                                              aGGTATTAGCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCCTTGAATGTAAATTACGCGTTAACAGCGCAGAACTCACGCAGGATGTTTGa            >  1:915794/1‑143 (MQ=255)

                                                                                                                                 tATTAGCCTTCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGAcgc         <  1:801148/143‑1 (MQ=255)

                                                                                                                                      gCCTGCTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTCAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATGGCATGTAAATTCCGCGTTACCAGCGCAGAACCCCAACATGATGTTTCAcgagtccg    >  2:270102/1‑137 (MQ=255)

                                                                                                                                        cTGCTCAGGTTCCGCAGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc  <  1:889437/143‑1 (MQ=255)

GTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCCTTCGCAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGC  >  NC_000913/4196080‑4196358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A