breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
RA 135,167 G→T T139N (ACC→AAC)  speD ← S‑adenosylmethionine decarboxylase proenzyme
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 674,768 G→T R6S (CGC→AGC)  leuS ← leucine‑‑tRNA ligase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 1,151,736 T→C V41A (GTT→GCT)  acpP → acyl carrier protein
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC JC 1,572,474 IS5 (+) +4 bp coding (2726‑2729/2796 nt) pqqL ← putative zinc peptidase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,737,631 +A coding (33/48 nt) pheL → phe operon leader peptide
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,033,610 G→A G156S (GGT→AGT)  trkH → K(+) transporter TrkH
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,219 A→C intergenic (‑120/+113) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1209603 1209618 16 54 [0] [0] 49 stfE e14 prophage; putative side tail fiber protein fragment
* * ÷ NC_000913 3423729–3424543 3424543 1–815 24 [22] [23] 24 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 566777 =NA (NA)2589 (43.020) 270/280 0.0 97.9% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 56 (0.920)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 57247156 (0.920)20 (0.430)
+34 bp
15/214 2.3 36.4% coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
?NC_000913 = 2170235 36 (0.590)noncoding (65/1258 nt) IS3 repeat region
* ? NC_000913 1207790 =2 (0.030)42 (0.780) 37/250 0.3 97.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 0 (0.000)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078053 (0.050)48 (0.890) 41/250 0.2 97.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 0 (0.000)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1411899 =88 (1.450)62 (1.250) 47/230 0.0 49.6% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 54 (1.090)pseudogene (51/51 nt) ttcC putative tRNA 2‑thiocytidine (s(2)C32) synthase