Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F91 I1 R1	26	61.7	2321240	90.8%	2107685	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	695,766	G→A	W222* (TGG→TGA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	695,766	0	G	A	98.0%	145.7 / NA	51	W222* (TGG→TGA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/28); minor base C (0/1); total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTC  >  NC_000913/695626‑695905

ggAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGc                                                                                                                                           <  2:287645/143‑1 (MQ=255)

 gAATTGGCGTTCATGCATAGCAGTATCGGCAGTTTTGCATGTTGCTTAGAGTACAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTGGTTGGATGACTGAGCa                                                                                                                                          >  1:438307/1‑143 (MQ=255)

    ttGGCGTTTGTGCCTGCCAGTTGGCGCCGGCGTGCATGTTGACTAGCGTCCAGTGCGAGAACGCTCCCGGCGACAGCACTTGGCAGCAATTTTCTTCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGATCATCg                                                                                                                                       <  2:474078/143‑1 (MQ=255)

    ttGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCg                                                                                                                                       >  1:564834/1‑143 (MQ=255)

            catgcgtgGCAGTATGCGCAGTCGTGCCTGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGtgtg                                                                                                                               <  2:442695/143‑1 (MQ=255)

            catgcatgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGtgtg                                                                                                                               <  2:48498/143‑1 (MQ=255)

                  tgGCGGTCTGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa                                                                                                                         <  2:555172/143‑1 (MQ=255)

                  tgGCGGTAGGCGCAGTCGTTCATGTTGATTAGCTTCCAGTACGAGATCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa                                                                                                                         <  2:555002/143‑1 (MQ=255)

                  tgGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa                                                                                                                         <  1:1155745/143‑1 (MQ=255)

                  tgGCAGCATGCGCCGTCGTGCATGTTGGTTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCTCCTTGCAGCAATTTACGCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGa                                                                                                                         <  2:1105122/143‑1 (MQ=255)

                        gaTGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGTGCATCGCTGGTGTGGTATGACTCTcc                                                                                                                   <  2:309889/142‑1 (MQ=255)

                           gcgcAGTCGGGCATGTTGGTTCTCGTCCAGTGCGAGGACGACCCCGGCGACAGCACCGGGCGGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGgc                                                                                                                <  2:97796/143‑1 (MQ=255)

                             gcAGGCGTGCATGGTGATGAGCGTCCAGGGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGgcgc                                                                                                              <  1:236797/143‑1 (MQ=255)

                               actCGTGCATGTTGACCAGCGTCCACCGCGAGAACGCTCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGt                                                                                                            <  1:235470/141‑1 (MQ=255)

                                     gCATGTTGATTCGCGGCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGc                                                                                                      <  2:322609/143‑1 (MQ=255)

                                       aTGTTGCTGAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGCTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCa                                                                                                    <  1:1040155/143‑1 (MQ=255)

                                          ttGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGtt                                                                                                 >  1:443091/1‑143 (MQ=255)

                                                aGCGTCTAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaa                                                                                           <  1:777457/143‑1 (MQ=255)

                                                aGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaa                                                                                           >  1:622450/1‑143 (MQ=255)

                                                  cgtgccgTGCGAGAGCGATCGCGGCGACGGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCGGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaata                                                                                         <  2:642786/137‑1 (MQ=255)

                                                  cGTCCAGTGCGAGAGCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaata                                                                                         <  1:40453/143‑1 (MQ=255)

                                                   gTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatat                                                                                        <  2:464604/143‑1 (MQ=255)

                                                         tGCGAGATGCATCCGGGCGACAGCAGGTGGCAGCAATTTACGCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaatat                                                                                  <  1:572977/143‑1 (MQ=255)

                                                         tGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGTACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAgaatatgaatat                                                                                  <  1:679261/143‑1 (MQ=255)

                                                               aaCGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGAACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGCATTCGCCAGTTGCAGAATATGAATATGGcaca                                                                            >  1:189057/1‑143 (MQ=255)

                                                                    tCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATGTGAATATGGCACAGCTcc                                                                       >  1:274678/1‑143 (MQ=255)

                                                                      ccGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAg                                                                     >  1:515298/1‑143 (MQ=255)

                                                                                   cTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGa                                                        >  1:762214/1‑143 (MQ=255)

                                                                                   cTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGa                                                        <  2:443091/143‑1 (MQ=255)

                                                                                         gcaATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGCAGCAtg                                                  >  1:601980/1‑142 (MQ=255)

                                                                                         gcaATTGTCTCCGGACGGACCGCGTGCGTGTCTGCCGTTGTTTGATAACGGAGCATCGCTGGTGTGGTAGGACTCTCCGGCGCGGATTCGCCCGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCAtt                                                  <  2:285349/143‑1 (MQ=255)

                                                                                           aaTTTACTCCGGACGGACCGCGTGCATTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGCGTATGGCACAGCTCCAGGCGGAGATGGCGAGGCATTTc                                                >  1:71959/1‑143 (MQ=255)

                                                                                           aaTTTACGCCTGGCGGACCGCGGGCGTGTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCTCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTc                                                <  2:711069/143‑1 (MQ=255)

                                                                                               tACTCCGGACGGACCGCGGGCGTTTCTGCCGGTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGt                                            <  1:455295/143‑1 (MQ=255)

                                                                                                  tCCGGACGTACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGTGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTcgc                                         <  1:23665/143‑1 (MQ=255)

                                                                                                  tCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCAGGTGTGGTATGACTCAACGGCGCGTATTAGCCAGTTGCAGAATATGAATATGGCAACGCTCCAGGCGGAAAGCGCGGAGCAGTTCACGTcgg                                         >  1:285956/1‑142 (MQ=255)

                                                                                                  tCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGAGAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTcgc                                         >  2:348448/1‑143 (MQ=255)

                                                                                                    cggacggaCCGCGTGCGTTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGt                                       >  1:966308/1‑143 (MQ=255)

                                                                                                        cggacCGCGTGCGTGTCTGCCGGTGCATGATATATCAGCACCGGGGTTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTgg                                   <  1:482718/143‑1 (MQ=255)

                                                                                                        cgcacCGCGTCCGTTCCTGCCGTTGTTTTAAAACTGCGCATCGTTGGTGTGGTATGACTCTCCGGCGCATATTCGCCAGTTGAAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCTAAGCATTTCCCGTCGCGTCTgg                                   <  1:885021/140‑1 (MQ=255)

                                                                                                               cgtgcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGGCGCGTCGGGggtacgt                            >  2:365921/1‑143 (MQ=255)

                                                                                                                 tgcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGgttacgtta                          >  1:521253/1‑143 (MQ=255)

                                                                                                                 ggcgtTTCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGgttacgtta                          <  1:705988/142‑1 (MQ=255)

                                                                                                                     tttCTGCCTTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACAcc                      <  1:551857/143‑1 (MQ=255)

                                                                                                                     tttCTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACAcc                      >  2:1068750/1‑143 (MQ=255)

                                                                                                                        cTGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCt                   >  1:919010/1‑143 (MQ=255)

                                                                                                                         tGCCGTTGTTTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGAGTCTGGGTTAGGTTACACCGGtt                  >  2:717822/1‑143 (MQ=255)

                                                                                                                              ttgttTGATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTCGGTTACGTTACACCGCTTGCCGc             >  1:1006421/1‑143 (MQ=255)

                                                                                                                               tgttTGATAACTGAGCATCGCTGGTGTGGTATGCCTCTCCGCCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCt            <  1:922066/143‑1 (MQ=255)

                                                                                                                                    gATAACTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCAGTTCCCGTCGCGTCTGGGTTACGCGACACCGCTCGCCGCTGGTGc       >  2:673121/1‑143 (MQ=255)

                                                                                                                                         cTGAGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTc  >  1:317945/1‑143 (MQ=255)

GGAATTGGCGTTCATGCATGGCAGTATGCGCAGTCGTGCATGTTGATTAGCGTCCAGTGCGAGAACGATCCCGGCGACAGCACCTGGCAGCAATTTACTCCGGACGGACCGCGTGCGTTTCTGCCGTTGTTTGATAACTGGGCATCGCTGGTGTGGTATGACTCTCCGGCGCGTATTCGCCAGTTGCAGAATATGAATATGGCACAGCTCCAGGCGGAAATCGCGAAGCATTTCCCGTCGCGTCTGGGTTACGTTACACCGCTTGCCGCTGGTGCGTTTC  >  NC_000913/695626‑695905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A