breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 125,387 C→T Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
RA 695,766 G→A W222* (TGG→TGA ubiF → 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 710,595 G→T P18T (CCT→ACT)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 759,250 G→T R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 800,807 G→T A17S (GCG→TCG)  ybhH → putative isomerase YbhH
RA 962,670 A→G K226E (AAA→GAA)  rpsA → 30S ribosomal subunit protein S1
RA 1,019,309 C→A D249Y (GAT→TAT)  ompA ← outer membrane porin A
RA 1,237,405 G→T intergenic (‑164/‑166) ycgB ← / → dadA PF04293 family protein YcgB/D‑amino acid dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,934,862 G→A R485C (CGT→TGT)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,661,969 C→G V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,632 T→A V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,646 +T coding (5/1500 nt) pitA → metal phosphate:H(+) symporter PitA
MC JC 3,815,859 Δ82 bp [rph] [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,114,431 G→A N14N (AAC→AAT fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,123,110 G→A P77L (CCA→CTA)  ftsN ← cell division protein FtsN
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423727–3424234 3424544–3424238 5–818 28 [27] [27] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)58 (0.970) 47/266 0.2 100% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 566777 =NA (NA)2060 (32.690) 269/280 0.0 97.8% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 47 (0.740)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =7 (0.110)45 (0.800) 39/250 0.4 83.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 12 (0.210)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078058 (0.130)39 (0.690) 36/250 0.5 80.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 12 (0.210)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment