Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F91 I1 R1
|
26 |
61.7 |
2321240 |
90.8% |
2107685 |
142.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
962,670 |
A→G |
K226E (AAA→GAA) |
rpsA → |
30S ribosomal subunit protein S1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 962,670 | 0 | A | G | 98.2%
| 167.4
/ NA
| 55 | K226E (AAA→GAA) | rpsA | 30S ribosomal subunit protein S1 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (29/24); minor base T (1/0); total (31/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGCAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGC > NC_000913/962530‑962806
|
gcgcAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGgaa > 2:16074/1‑143 (MQ=255)
aTCAGCCGCCGGAACACCTGCCGGAAGGCATGGCAGTTAAAGGTATCGTTAATAACCTCACTGACTACGGTGCATTCGTTGATCCTGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCAt < 1:453305/143‑1 (MQ=255)
gctgctGGGAATGCTGCAGGAAGGCATGGATGGTGAAGGGATGGTGAGGAACCTCACTGACTACGGTGCAGTCGTTGATCTGGGCGGCGTTGACGTCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGa < 2:222029/143‑1 (MQ=255)
tggtgGAAAACCTGCGGGAAGGCATTGAAGTTAAACGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGc < 1:197378/140‑1 (MQ=255)
tgctgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGc < 2:154037/143‑1 (MQ=255)
gctgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCg > 1:287135/1‑143 (MQ=255)
tgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGACCGaa > 2:872490/1‑143 (MQ=255)
cTGCAGGAAGGCATGGAAGTTACAAATATGTGGAAGAACCTCACTTTCTTGTGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa < 1:1031707/143‑1 (MQ=255)
cTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa > 1:609185/1‑143 (MQ=255)
cTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCaa < 1:925307/143‑1 (MQ=255)
caaGGCACGGAAGTCACACGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTggg < 1:966468/142‑1 (MQ=255)
gCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATCACCTCGTTCAAAACTAAGGATCCGAGCGAAATCGTCAACGTGGGagac > 2:239524/1‑143 (MQ=255)
cagggAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGcgacg < 1:554840/140‑1 (MQ=255)
aTGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGACTGCTGCCCATCACTGACATGGCCTGGGAACGCGTTAGGCATCCGAGCGAAATCGTCAACGTGGGcgacga > 1:216758/1‑143 (MQ=255)
gTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCAc < 1:471644/143‑1 (MQ=255)
ttAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAACGAAATCGTCAACGTGGGCGACGAAATCACt > 2:143170/1‑143 (MQ=255)
ttAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTAGTTGGTCTGGGCGGCGTTGACGGCCTGCTGCACGTCACTGACATGGCCTGGGGACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGGAATcgat > 2:147265/1‑140 (MQ=255)
aaaGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGt > 1:672986/1‑143 (MQ=255)
ggTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTaa > 1:295637/1‑143 (MQ=255)
gTATCGTTAAGAACCTCACTGACTACGGAGCATTCGTTTATCTGTGAGGCATTGACGGCCTGCTGCACATCACTGACTTGGCCTGGCAACGAGTTAATCGTCCGAGCGAAGTCGTCAACGTGGGCGGCGAACTCACAGttgac > 2:1010073/1‑140 (MQ=255)
cGTTAAGACGCTCACTGAGTACGGTGCATTCGTTGCTCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGc < 1:1012158/143‑1 (MQ=255)
aaCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGATAAAATGCTGAAGta > 1:973239/1‑142 (MQ=255)
aCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTc < 1:1050030/143‑1 (MQ=255)
aCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCAGCACATCACTGACATGGCCTGGGAACGCGTTAAGCGTCCGAGCGAAATCGTCAACGTAGCCGACGCAATCACAGGTAGAGTGGTGAAgagc > 1:127226/1‑140 (MQ=255)
ccTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTGAGCATCGGAGCGAAGTCGTCAACGTGGGCGACGAAATAACTGTTAAAGTGCTGAAGGTCg > 1:351612/1‑143 (MQ=255)
cTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa > 1:1021408/1‑143 (MQ=255)
cTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa < 1:165454/143‑1 (MQ=255)
tCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACCGTTAAAGTGCTGAAGTTCGAc > 1:356110/1‑143 (MQ=255)
aCTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCAGAGCGAAACCGTCAACGGGAGCGACGAAAGCACTAGTACAGTCCTGAAGCTCCACcg > 2:862753/1‑143 (MQ=255)
cTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAAc < 1:16074/143‑1 (MQ=255)
tACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACg > 1:498749/1‑143 (MQ=255)
aTTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGCGACCGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCgtg < 1:42102/143‑1 (MQ=255)
gATCTGGGCGGCCTTGACGGCCTGCTTCACATCTCCGACATGGCCTGCGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGTGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCt < 1:847870/143‑1 (MQ=255)
tCTGGGGGGCGTTGACGGCGTGGTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTgg < 1:399227/143‑1 (MQ=255)
ggcggcGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGGACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGACATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAACCGTGTACCCAGGGGCCt > 2:965795/1‑143 (MQ=255)
ggcggcGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCt > 2:486874/1‑143 (MQ=255)
gcggcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTg > 1:279213/1‑143 (MQ=255)
cggcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGa < 2:287135/143‑1 (MQ=255)
gcgTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGaaa < 2:242585/143‑1 (MQ=255)
tgctgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCATGGGCCTGGAACAGCTGGGCGaa > 2:1057569/1‑143 (MQ=255)
gctgcACATCCCTGAACTGGCCTGGGAACGCGTTAAGCATCCGAGTGAAATAGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAg < 1:842150/143‑1 (MQ=255)
ctgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCGGAGCGAAGa > 2:64681/1‑143 (MQ=255)
tgcACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGAt < 2:672986/143‑1 (MQ=255)
gaTCAGTGACTTGGCCTGGGAACGCGTTAAGCATCCGGGCGAAGTCGTCAGCGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAACCGTGTATCCCTGGGCCTGAAACAGCTGGGCGACGATCCGt < 2:1080489/142‑1 (MQ=255)
aTCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg > 1:378633/1‑143 (MQ=255)
aTCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg > 2:227696/1‑143 (MQ=255)
aTCACTGACATGGCCTGGGAACGCGTGAAGCATCCGAGCGAAATCGTCAACGTGGACGACGAAATCACTGTTAACGTGCTGAAGTTCGACCGCGAACGTACCCTTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTg > 2:1105187/1‑143 (MQ=255)
tCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGCACCAGCTGGGcgacgacaccgtt > 1:836408/1‑136 (MQ=255)
tCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTgg < 1:1123268/143‑1 (MQ=255)
aCATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCt > 2:807854/1‑143 (MQ=255)
aTGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTAt < 1:664340/143‑1 (MQ=255)
gggcTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAGCGATAGATGGATTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCg < 1:534703/140‑1 (MQ=255)
ggCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGCTCCGTGGGTCGCTACCg > 2:725505/1‑143 (MQ=255)
ggCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGACGACGATCCGTGGGTCGCGATCg > 2:585556/1‑143 (MQ=255)
gCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGGAGATCCGTGGGTAGCTGTCGc > 2:366914/1‑143 (MQ=255)
gCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGCTCGGTGGGTAGCTATCGc > 1:693021/1‑143 (MQ=255)
|
GCGCAGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGC > NC_000913/962530‑962806
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
