Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F66 I1 R1	33	61.7	2789148	95.4%	2660847	127.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,097,087	(A)_7→8	intergenic (‑111/+39)	azoJ ← / ← yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,097,080	1	.	A	92.4%	205.5 / 10.8	66	intergenic (‑104/+46)	azoJ/yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein
Reads supporting (aligned to +/- strand): ref base . (2/3); new base A (28/33); total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

TCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑C‑AAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAC  >  BS168_reference_genome/2096945‑2097212

tCGCTTGATTTTAAAAATAAAACTGTATACATTTTTATTACTCCCGGTAAACTTTTTATATTTAATTACAAATAGTATTTTAATTTCTTTTTTAAGTAAAGTAAATCACTTTATTTTTAATATTAGATTTTTTTT‑C‑aa                                                                                                                                      >  3:347277/1‑138 (MQ=255)

  gCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑C‑aaaaa                                                                                                                                   <  1:13552/139‑1 (MQ=255)

             cAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTa                                                                                                                        <  3:51715/138‑1 (MQ=255)

             cAAATAAAACTGTAGACATTTGTATTCCTCACGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTa                                                                                                                        <  7:229919/138‑1 (MQ=255)

                aTAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATc                                                                                                                    <  1:15564/139‑1 (MQ=255)

                aTAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATc                                                                                                                    <  4:40707/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCTGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAatat                                                                                                              <  8:161627/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAatat                                                                                                              <  4:283053/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAatat                                                                                                              <  3:9728/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAatat                                                                                                              <  5:155367/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAatat                                                                                                              <  1:91915/139‑1 (MQ=255)

                      cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑C‑AAAAATA‑AGCAAGTACATCTAatat                                                                                                              <  6:286896/139‑1 (MQ=255)

                         tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCgg                                                                                                           >  7:131281/1‑139 (MQ=255)

                         tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCgg                                                                                                           >  3:153481/1‑139 (MQ=255)

                           gACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGgtg                                                                                                         >  4:30701/1‑139 (MQ=255)

                                ttGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTActt                                                                                                    >  2:307501/1‑139 (MQ=255)

                                 tGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGATTATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGTTGTActtg                                                                                                   >  2:1230/1‑139 (MQ=255)

                                 tGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTActtg                                                                                                   >  2:338369/1‑139 (MQ=255)

                                     ttCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTActtgctt                                                                                                <  3:145620/138‑1 (MQ=255)

                                       cctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTgg                                                                                              <  7:287490/138‑1 (MQ=255)

                                        ctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAg                                                                                                                          <  6:192656/109‑1 (MQ=255)

                                        ctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAg                                                                                                                          >  5:192656/1‑109 (MQ=255)

                                        ctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCa                                                                                            >  5:81315/1‑139 (MQ=255)

                                          cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTa                                                                                                                        >  2:56427/1‑109 (MQ=255)

                                          cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTa                                                                                                                        <  1:56427/109‑1 (MQ=255)

                                          cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt                                                                                          >  3:187378/1‑139 (MQ=255)

                                          cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt                                                                                          >  3:341476/1‑139 (MQ=255)

                                             ggTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtttt                                                                                        >  2:247992/1‑138 (MQ=255)

                                                        tataTTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCt                                                                            >  7:93071/1‑139 (MQ=255)

                                                         atatTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCtt                                                                           >  8:89012/1‑139 (MQ=255)

                                                              tacttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                      >  2:180853/1‑139 (MQ=255)

                                                              tacttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                      >  4:166077/1‑139 (MQ=255)

                                                              tacttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                      >  3:214321/1‑139 (MQ=255)

                                                               acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                      <  1:307501/138‑1 (MQ=255)

                                                                    cAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                                <  8:93071/139‑1 (MQ=255)

                                                                      aaTAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCaa                                                              <  4:169943/139‑1 (MQ=255)

                                                                             tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTc                                                        <  5:172616/138‑1 (MQ=255)

                                                                             tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa                                                       >  4:68505/1‑139 (MQ=255)

                                                                               ttACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCGTGTTCACTATCCAAGATTTCATa                                                     >  2:333856/1‑139 (MQ=255)

                                                                                 aCTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACg                                                   >  7:109597/1‑139 (MQ=255)

                                                                                   tttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCTGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTc                                                 >  3:28423/1‑139 (MQ=255)

                                                                                   tttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTc                                                 >  6:211720/1‑139 (MQ=255)

                                                                                     tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACTTCTg                                               >  4:35068/1‑139 (MQ=255)

                                                                                     tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTg                                               >  2:114259/1‑139 (MQ=255)

                                                                                     tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTAATTGCTTGGCTTTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCTTACGTCTg                                               >  8:134889/1‑139 (MQ=255)

                                                                                         ttttACGTGAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAg                                           >  7:249375/1‑139 (MQ=255)

                                                                                         ttttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAGTTTTACTTCGAATCTTGGTCACGATCAAAGATTTCAAACGTCTGCGAg                                           >  8:342210/1‑139 (MQ=255)

                                                                                          tttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAg                                           <  8:263128/138‑1 (MQ=255)

                                                                                                  aaGTCAATCACTTTATTTTGAATATGAGATTTTTTTTAA‑AAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTg                                   <  1:338369/138‑1 (MQ=255)

                                                                                                  aaGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGa                                  <  4:187378/139‑1 (MQ=255)

                                                                                                        aTCACTTTATTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAAtt                            >  2:74772/1‑139 (MQ=255)

                                                                                                                aTTTTGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCtt                     <  4:214321/138‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  1:132897/139‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  8:109597/139‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  1:180853/139‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  6:87272/139‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  2:62900/139‑1 (MQ=255)

                                                                                                                 ttttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  3:57919/139‑1 (MQ=255)

                                                                                                                  tttGAATATGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                   <  4:28423/138‑1 (MQ=255)

                                                                                                                       atatGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGAt             <  5:211720/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  6:81315/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  5:80791/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  3:328734/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTTT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  5:185872/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTTT‑C‑AAAAA‑ATAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  1:247992/139‑1 (MQ=255)

                                                                                                                        tatGAGATTTTTTAT‑CAAAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa            <  4:69641/139‑1 (MQ=255)

                                                                                                                               tttttttc‑A‑AAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCttt     >  4:73012/1‑138 (MQ=255)

                                                                                                                                 tttttc‑A‑AAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAc  >  2:336028/1‑139 (MQ=255)

TCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTT‑C‑AAAAA‑A‑AGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAC  >  BS168_reference_genome/2096945‑2097212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A