Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 29 281.6 8141248 99.5% 8100541 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 2,097,087 (A)7→8 intergenic (‑111/+39) azoJ ← / ← yocK FMN‑dependent NADH‑azoreductase/putative general stress protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,097,0801.A91.8% 623.1 / 48.5 182intergenic (‑104/+46)azoJ/yocKFMN‑dependent NADH‑azoreductase/putative general stress protein
Reads supporting (aligned to +/- strand):  ref base . (8/7);  new base A (74/93);  total (82/100)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

Only 100 of 198 total aligned reads displayed.

CTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAA  >  BS168_reference_genome/2096931‑2097224
                                                                                                                                                       |                                                                                                                                                   
cttcAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAATTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTc                                                                                                                                                      <  1:899478/150‑1 (MQ=255)
cttcAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTc                                                                                                                                                      >  2:3535066/1‑150 (MQ=255)
 ttcAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACCTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGCCAATCACTTTATTTTGAATAGGAGATT‑TTTTTTC‑a                                                                                                                                                    <  1:2762118/150‑1 (MQ=255)
    aGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aa                                                                                                                                                >  2:3116676/1‑150 (MQ=255)
      cTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aaaa                                                                                                                                              <  1:3433870/150‑1 (MQ=255)
        gTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aaaa‑aa                                                                                                                                           >  2:276921/1‑149 (MQ=255)
           cGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aaaa‑aagca                                                                                                                                        >  1:2173842/1‑146 (MQ=255)
            ggTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aaaa‑aagcaa                                                                                                                                       >  2:2225984/1‑145 (MQ=255)
            ggTCGCTTGATTTTACAAACAAAACTGTAGACATCTGTATTGCCCGCGGTACACTTTTTATATTTACTTACAAATAGTATGTTACTTGCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑aa‑aaaa‑aagcaa                                                                                                                                       <  1:2836377/150‑6 (MQ=255)
             gTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAg                                                                                                                                      <  1:574998/150‑1 (MQ=255)
              tCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGt                                                                                                                                     <  2:1507135/150‑1 (MQ=255)
               cGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGGTT‑TTTTTTCAAA‑AAAA‑A‑GCAAgaa                                                                                                                                    >  2:825244/1‑148 (MQ=255)
                 cTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACa                                                                                                                                  <  1:2913645/150‑1 (MQ=255)
                   tgtttttACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTCTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATc                                                                                                                                <  1:2803105/147‑1 (MQ=255)
                    gATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCt                                                                                                                               <  1:3993072/150‑1 (MQ=255)
                     aTTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTa                                                                                                                              >  2:1832806/1‑150 (MQ=255)
                      ttttACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTaa                                                                                                                             >  1:1655528/1‑150 (MQ=255)
                      ttttACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTaa                                                                                                                             >  1:1161555/1‑150 (MQ=255)
                       tttACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAat                                                                                                                            <  1:37327/150‑1 (MQ=255)
                          aCAAATAAGACTGTGGGCATTTGTCTTCCACCCGGTAACCTTTTTATATGTACGTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCCCTTTATTTTGAATATGAGATT‑TTTTCTCAAA‑AAAA‑A‑GCAAGTACATCTAATATc                                                                                                                         <  2:1353184/150‑1 (MQ=255)
                              aTAAGACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTAGGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGgtg                                                                                                                     <  2:3932969/150‑1 (MQ=255)
                                  aaCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTAct                                                                                                                 <  2:1161555/150‑1 (MQ=255)
                                  aaCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTAct                                                                                                                 <  1:1386721/150‑1 (MQ=255)
                                     tGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTActtgc                                                                                                              >  1:1231022/1‑150 (MQ=255)
                                       tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTActtgctt                                                                                                            <  1:3451398/150‑1 (MQ=255)
                                       tAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAA‑AGGCAAGTACAACTAATATCGGTGTActtgctt                                                                                                            >  1:3566834/1‑150 (MQ=255)
                                           cATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCa                                                                                                        <  2:1859878/150‑1 (MQ=255)
                                                gTATTCCTCCCGGTAAACTTCTTATATTTTCTTTCAAATAGTATGTTCCTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCAttttt                                                                                                   <  2:3976208/150‑1 (MQ=255)
                                                 tATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTa                                                                                                  >  1:3272253/1‑150 (MQ=255)
                                                  aTTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTAc                                                                                                 >  1:1987916/1‑150 (MQ=255)
                                                  aTTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTAACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTAc                                                                                                 <  1:316353/150‑1 (MQ=255)
                                                   ttCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACt                                                                                                <  1:2528921/150‑1 (MQ=255)
                                                    cccccccGGTAAACTTTTTATATTTTCTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACtt                                                                                               <  2:1160312/146‑1 (MQ=255)
                                                     cctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTc                                                                                              >  2:462102/1‑150 (MQ=255)
                                                         ccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACGTGCTTGGCATTTTTACTTCGAAt                                                                                          >  2:1311740/1‑150 (MQ=255)
                                                           ggTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCt                                                                                        <  2:3044458/150‑1 (MQ=255)
                                                            gTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCtt                                                                                       >  1:1430917/1‑150 (MQ=255)
                                                               aaCTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGtt                                                                                    >  2:1395522/1‑150 (MQ=255)
                                                                tcTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTCCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTc                                                                                   <  2:2960014/149‑1 (MQ=255)
                                                                 cTTTTTATTTTTCCTTACAAATAGTATGTTACTTTCTTTTCTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAA‑ATGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                                  <  1:3691943/150‑1 (MQ=255)
                                                                 cTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                                                  <  2:211021/150‑1 (MQ=255)
                                                                 cTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCAATTTTACTTCGAATCTTGGTCa                                                                                  >  2:1535952/1‑150 (MQ=255)
                                                                  tttttATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCAc                                                                                 <  2:1480305/150‑1 (MQ=255)
                                                                   ttttATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACg                                                                                <  1:3092718/150‑1 (MQ=255)
                                                                      tattTTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATc                                                                             <  2:3772469/150‑1 (MQ=255)
                                                                       atatTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                                            >  1:3640430/1‑150 (MQ=255)
                                                                       atatTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                                            <  1:2032728/150‑1 (MQ=255)
                                                                       atatTTACTTACAAATAGTATGTAACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                                            <  1:2260731/150‑1 (MQ=255)
                                                                         atTTACTTACAAATAGTATGTTACATTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCaa                                                                          <  1:865106/150‑1 (MQ=255)
                                                                          tttCCTTCCAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAg                                                                         <  2:1469889/150‑1 (MQ=255)
                                                                            tacttacAAATAGTATGTTACTTTCTTTTTTACGTGAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGAt                                                                       <  1:2674416/150‑1 (MQ=255)
                                                                             acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGAtt                                                                      <  1:320285/150‑1 (MQ=255)
                                                                                  cAAATAGTATGTTACTTTCATTTTTACATAAAGTCAATCACTTTATTTTGATTATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATa                                                                 <  1:2335874/150‑1 (MQ=255)
                                                                                   aaaTAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATAc                                                                <  2:2345781/150‑1 (MQ=255)
                                                                                    aaTAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACg                                                               <  2:44702/150‑1 (MQ=255)
                                                                                      tAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTc                                                             <  1:1395522/150‑1 (MQ=255)
                                                                                       aGTATGTTCCTTTCTTTTTTACGTAAAGCCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCt                                                            <  2:323314/150‑1 (MQ=255)
                                                                                       aGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGGTCCAAGATTTCATACGTCt                                                            >  2:736879/1‑150 (MQ=255)
                                                                                       aGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCt                                                            <  1:3530731/150‑1 (MQ=255)
                                                                                          aTGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCg                                                         <  2:2640056/150‑1 (MQ=255)
                                                                                          aTGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCg                                                         >  2:419307/1‑150 (MQ=255)
                                                                                            gTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTACAAA‑AAAA‑A‑GCAAGTAAATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAg                                                       <  1:1907296/150‑1 (MQ=255)
                                                                                             ttACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGGCTGCGAGc                                                      >  2:2262114/1‑150 (MQ=255)
                                                                                                ctttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCttt                                                   >  1:887119/1‑150 (MQ=255)
                                                                                                ctttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCttt                                                   >  1:763611/1‑150 (MQ=255)
                                                                                                 ttttttttttACGTAAAGTCAATCATTTTATTTTGAATATGAGATTCGTTATGA‑AA‑AAAA‑A‑GCATGTACATCTAATATCGGTGTTATTGCTTGGTATTTTTACTTCGAATCTTGTTCACGATTCAAGATTTCATACGTCTGCGAGCtatt                                                  <  1:2717905/150‑1 (MQ=255)
                                                                                                 tttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCtttt                                                  <  1:528238/150‑1 (MQ=255)
                                                                                                      tttttACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGAt                                             <  1:915306/150‑1 (MQ=255)
                                                                                                      tttttACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGAt                                             <  1:698093/150‑1 (MQ=255)
                                                                                                       ttttACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATc                                            <  2:2816583/150‑1 (MQ=255)
                                                                                                        tttACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGTTCa                                           >  2:3278947/1‑150 (MQ=255)
                                                                                                         ttACGTGAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAACA‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCaa                                          <  2:1784202/150‑1 (MQ=255)
                                                                                                          tACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAAt                                         <  1:2643145/150‑1 (MQ=255)
                                                                                                             gTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGt                                      <  2:84131/150‑1 (MQ=255)
                                                                                                             gTAAAGTCAATCACTTCATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACACCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGt                                      <  1:1688745/150‑1 (MQ=255)
                                                                                                               aaaGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTATTTGATCAATTGttt                                    >  1:1404389/1‑150 (MQ=255)
                                                                                                               aaaGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTc                                   >  2:2062366/1‑150 (MQ=255)
                                                                                                                 aGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCt                                  >  1:2487876/1‑150 (MQ=255)
                                                                                                                  gTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCtt                                 >  2:3453933/1‑150 (MQ=255)
                                                                                                                    acttCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AACAAAA‑A‑GCAAGTTCATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg                               <  1:2709162/147‑1 (MQ=255)
                                                                                                                       tCACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttgttg                            >  2:1380721/1‑150 (MQ=255)
                                                                                                                        cACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTgg                           <  2:3133136/150‑1 (MQ=255)
                                                                                                                        cACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTgg                           >  2:3354530/1‑150 (MQ=255)
                                                                                                                        cACTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGGGTACTTGCTTGGCATTTTTACTTCGAATCTTGGTCACGATCCAAGATTTCATACGGCTGCGAGCCTGTTTGATCAATTGTTTCTTTGCTgg                           >  2:2709162/1‑150 (MQ=255)
                                                                                                                         aCTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGa                          >  2:3643627/1‑150 (MQ=255)
                                                                                                                          cTTTATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGAt                         <  2:524697/150‑1 (MQ=255)
                                                                                                                           ttttttttGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTTTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTTTTTGTTGGATa                        <  1:2138847/150‑1 (MQ=255)
                                                                                                                           tttATTTTGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa                        >  2:2045445/1‑150 (MQ=255)
                                                                                                                                 ttGAGTATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCtt                  <  2:1547936/150‑1 (MQ=255)
                                                                                                                                  tGAATATGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCttt                 <  2:2419543/150‑1 (MQ=255)
                                                                                                                                     atatGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAc              <  1:391433/150‑1 (MQ=255)
                                                                                                                                      tatGAGATT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTAcc             >  2:4066199/1‑150 (MQ=255)
                                                                                                                                         gagtTT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTCCATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGATTCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGt          <  1:3225478/150‑1 (MQ=255)
                                                                                                                                         gagtTT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAACATCGGTGTACTTGCTTGGCGTTTTGACTTCGAATCTTGTCCACGAGCCAAGATTTCATACGTATGCGAGCTTTATTGATCAATTGTTTCCTTGATGGATAAGTCTTTTACCTGt          >  2:2391679/1‑150 (MQ=255)
                                                                                                                                         gagaTT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGt          <  1:3116676/150‑1 (MQ=255)
                                                                                                                                            aTT‑TTTTTTCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCa       <  2:3526390/150‑1 (MQ=255)
                                                                                                                                             tt‑ttttttCAAA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGTTCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCCTTTACCTGTTCAt      >  2:3803130/1‑150 (MQ=255)
                                                                                                                                             tt‑tttttcA‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATg     >  1:830993/1‑150 (MQ=255)
                                                                                                                                             tt‑tttttcA‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATg     >  1:3131577/1‑150 (MQ=255)
                                                                                                                                                tttttcA‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGaa   <  2:1729124/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CTTCAGCTGTACGGTCGCTTGATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATT‑TTTTTTC‑AA‑AAAA‑A‑GCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATAAGTCTTTTACCTGTTCATGAAA  >  BS168_reference_genome/2096931‑2097224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A