Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F66 I1 R1
|
37 |
65.5 |
2715618 |
95.2% |
2585268 |
125.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
BS168_reference_genome |
2,097,087 |
(A)7→8 |
intergenic (‑111/+39) |
azoJ ← / ← yocK |
FMN‑dependent NADH‑azoreductase/putative general stress protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | BS168_reference_genome | 2,097,080 | 1 | . | A | 95.3%
| 128.2
/ 0.5
| 43 | intergenic (‑104/+46) | azoJ/yocK | FMN‑dependent NADH‑azoreductase/putative general stress protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (20/21); total (20/23) |
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01 |
ATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑AAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATA > BS168_reference_genome/2096952‑2097202
|
aTTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑aaaaa‑aaagc < 7:303179/139‑4 (MQ=255)
tACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAg < 1:324626/138‑1 (MQ=255)
aCAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGt < 5:230137/138‑1 (MQ=255)
aCAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑AAAAATAAGCAAGt < 8:229054/138‑1 (MQ=255)
cAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTa < 7:28663/138‑1 (MQ=255)
aaaTAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACa < 4:262066/139‑1 (MQ=255)
aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑AAAAA‑AAGCAAGTACATCTAatat < 2:57337/139‑1 (MQ=255)
cTGTAGACATTTGTATTGCTCCCGGTATACTTTTTATATTTACTTACAAATAGTTTGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAatat < 3:156195/139‑1 (MQ=255)
cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAatat < 8:142912/139‑1 (MQ=255)
tATTCCTCCCGGTAAACATTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTAGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTCTActtgct > 3:107817/1‑139 (MQ=255)
ctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCa > 1:59366/1‑139 (MQ=255)
cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt > 7:112220/1‑139 (MQ=255)
cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt > 7:143734/1‑139 (MQ=255)
ggTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGTTGTACTTGCTTGGCAtttt > 7:39006/1‑138 (MQ=255)
ttacttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGtt > 4:70383/1‑138 (MQ=255)
acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa < 7:33123/138‑1 (MQ=255)
acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa < 3:145011/138‑1 (MQ=255)
tATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGAttt > 5:68988/1‑139 (MQ=255)
tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtttt > 5:222258/1‑106 (MQ=255)
tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtttt < 6:222258/106‑1 (MQ=255)
tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa > 4:89543/1‑139 (MQ=255)
tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa > 6:178165/1‑139 (MQ=255)
tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa > 6:182635/1‑139 (MQ=255)
aCTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACg > 5:221168/1‑139 (MQ=255)
tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa > 2:140743/1‑116 (MQ=255)
tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa < 1:140743/116‑1 (MQ=255)
tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTg > 6:12217/1‑139 (MQ=255)
tttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAg < 5:259769/138‑1 (MQ=255)
aaaGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCtttttt > 4:330043/1‑138 (MQ=255)
aTCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATccgat > 6:326012/1‑135 (MQ=255)
aTCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAttt > 3:25850/1‑139 (MQ=255)
aTCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAAtt > 2:215224/1‑139 (MQ=255)
tATTTTGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCtt > 1:270615/1‑139 (MQ=255)
ttttGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg < 5:182635/139‑1 (MQ=255)
ttttGAATATGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg < 5:178165/139‑1 (MQ=255)
atatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGa > 4:29163/1‑138 (MQ=255)
tattcgaTTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTTGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 5:72837/134‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 7:312008/139‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 4:119334/139‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 3:177676/139‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 3:143420/139‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 8:39006/139‑1 (MQ=255)
tatGAGATTTTTTTTCAAAAAA‑AAGCAAGGACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa < 6:140589/139‑1 (MQ=255)
atGAGATTTTTTTTCAAAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATTCGTCTGCGAGCTTTTTTGATCATTTGTTTCTTTGTTGGTTa < 3:111671/138‑1 (MQ=255)
|
ATTTTACAAATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑AAAAA‑AAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATA > BS168_reference_genome/2096952‑2097202
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
