Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F23 I1 R1	1	128.4	4471334	98.5%	4404263	136.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,176,460	A→G	K395E (AAA→GAA)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,176,460	0	A	G	97.8%	647.2 / 2.6	180	K395E (AAA→GAA)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base A (3/1); new base G (83/92); total (87/93)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 181 total aligned reads displayed.

TCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176325‑4176593

tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTgaacc                                                                                                                                   >  1:1019334/1‑138 (MQ=255)

     tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  2:83124/1‑140 (MQ=255)

     tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCg                                                                                                                               <  1:1466143/139‑1 (MQ=255)

     tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCg                                                                                                                               >  2:1035686/1‑139 (MQ=255)

        ttCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAAACGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaaga                                                                                                                           >  1:2053139/1‑140 (MQ=255)

         tCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAGCTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaagaa                                                                                                                          >  1:721498/1‑140 (MQ=255)

                  aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGa                                                                                                                 >  1:234615/1‑140 (MQ=255)

                       aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCCTTGAAGCTGCGAAGAAAATGGTTGAGcgcg                                                                                                            >  1:1485749/1‑140 (MQ=255)

                            cATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCgaagac                                                                                                       >  2:1161129/1‑139 (MQ=255)

                            cATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCgaagaa                                                                                                       >  2:1756141/1‑140 (MQ=255)

                              tACCTGCCTATGCATCAGTGCGGTCTGCAGAAGAAAATGTCACTGGAGCTGTTCAAAGCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  2:849323/1‑140 (MQ=255)

                              tACCTGAGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     <  1:581449/140‑1 (MQ=255)

                               aCCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                                                                    >  1:479878/1‑140 (MQ=255)

                                ccTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTg                                                                                                   <  1:920254/140‑1 (MQ=255)

                                    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGACGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:529612/1‑140 (MQ=255)

                                    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  2:2219858/1‑140 (MQ=255)

                                    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               <  1:422330/140‑1 (MQ=255)

                                    cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:1337480/1‑140 (MQ=255)

                                        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                                                                    >  1:1348534/1‑131 (MQ=255)

                                        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                                                                    <  2:1348534/131‑1 (MQ=255)

                                        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:1666046/1‑140 (MQ=255)

                                        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:1691133/1‑140 (MQ=255)

                                        tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:676178/1‑140 (MQ=255)

                                         gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTCGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  2:2062333/140‑1 (MQ=255)

                                           aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGat                                                                                        >  2:530289/1‑140 (MQ=255)

                                                tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCt                                                                                   >  1:1579966/1‑140 (MQ=255)

                                                     tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACg                                                                              >  2:123486/1‑140 (MQ=255)

                                                         ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                                                          >  2:509133/1‑140 (MQ=255)

                                                          cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGtt                                                                         >  2:384732/1‑140 (MQ=255)

                                                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                  >  2:1604301/1‑140 (MQ=255)

                                                                  aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                 <  1:396100/140‑1 (MQ=255)

                                                                  aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                 <  1:270967/140‑1 (MQ=255)

                                                                   tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                >  1:1803408/1‑140 (MQ=255)

                                                                   tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                >  1:1316905/1‑140 (MQ=255)

                                                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                            <  1:1732941/140‑1 (MQ=255)

                                                                       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                            <  1:83124/140‑1 (MQ=255)

                                                                        cTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAaca                                                              >  2:1999295/1‑137 (MQ=255)

                                                                        cTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAaca                                                              <  1:1999295/137‑1 (MQ=255)

                                                                        cTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAaca                                                              <  1:1999578/137‑1 (MQ=255)

                                                                          ggATCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1560408/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:509133/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1949341/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:2223827/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:2213360/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:2141384/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:903314/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATAGCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:308444/140‑1 (MQ=255)

                                                                          ggAGCTGTTCAAACCGGTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1323767/140‑1 (MQ=255)

                                                                           gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGaagaag                                                                                                      >  2:1623886/1‑94 (MQ=255)

                                                                            aGCTGTTCAAAACGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  1:1395939/140‑1 (MQ=255)

                                                                                 ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                  >  2:395879/1‑140 (MQ=255)

                                                                                 gtCAAACCGTTCATCTACGGCAAGCGGGAACTGCGTGGGCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTGGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                  <  2:610335/139‑1 (MQ=255)

                                                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTTCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              <  2:1463697/140‑1 (MQ=255)

                                                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              <  2:1821138/140‑1 (MQ=255)

                                                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              <  1:1292390/140‑1 (MQ=255)

                                                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              <  1:841139/140‑1 (MQ=255)

                                                                                       ccGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCg                                            <  1:1026272/140‑1 (MQ=255)

                                                                                       ccGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCg                                            <  1:343565/140‑1 (MQ=255)

                                                                                           tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaaga                                                                                                                           <  1:1242132/57‑1 (MQ=255)

                                                                                           tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  2:748720/1‑140 (MQ=255)

                                                                                           tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  2:1190553/1‑140 (MQ=255)

                                                                                           tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  2:2128176/1‑140 (MQ=255)

                                                                                           tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  1:1840988/1‑140 (MQ=255)

                                                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:1001932/140‑1 (MQ=255)

                                                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:1190553/140‑1 (MQ=255)

                                                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:1609296/140‑1 (MQ=255)

                                                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:2053139/140‑1 (MQ=255)

                                                                                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:2119295/140‑1 (MQ=255)

                                                                                               cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGa                                    <  2:1961462/140‑1 (MQ=255)

                                                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                   <  1:699007/140‑1 (MQ=255)

                                                                                                  cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                 >  1:1053221/1‑108 (MQ=255)

                                                                                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                               <  1:384732/140‑1 (MQ=255)

                                                                                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTAGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                               <  1:2180309/140‑1 (MQ=255)

                                                                                                       aGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAc                            >  2:1255153/1‑140 (MQ=255)

                                                                                                            gAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                       >  1:1144479/1‑140 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                                                                    >  2:272336/1‑57 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               <  2:1320368/62‑1 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1125413/100‑1 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                             <  2:1422151/128‑1 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                             >  1:1422151/1‑128 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  1:2080255/1‑140 (MQ=255)

                                                                                                                  cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  1:1659346/1‑140 (MQ=255)

                                                                                                                     ggTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa              <  1:1729424/140‑1 (MQ=255)

                                                                                                                           gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGTAtt        <  2:721498/140‑1 (MQ=255)

                                                                                                                           gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt        <  2:1245384/140‑1 (MQ=255)

                                                                                                                           gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt        <  2:2115656/140‑1 (MQ=255)

                                                                                                                           gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt        <  2:1579966/140‑1 (MQ=255)

                                                                                                                             tACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg      <  2:234615/140‑1 (MQ=255)

                                                                                                                                caccaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc   <  1:1161129/140‑1 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  2:1521307/1‑85 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1521307/85‑1 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                      >  2:149949/1‑104 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1344434/1‑140 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:620203/1‑140 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:493624/1‑140 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1795687/1‑140 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1893706/1‑140 (MQ=255)

                                                                                                                                 accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1941674/1‑140 (MQ=255)

                                                                                                                                 accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                                                                 <  1:906723/61‑1 (MQ=255)

                                                                                                                                 accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                                                                 >  2:906723/1‑61 (MQ=255)

TCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176325‑4176593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A