Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F22 I1 R1 1 114.3 3996730 98.7% 3944772 135.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,176,460 A→G K395E (AAA→GAA)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,176,4600AG94.8% 537.1 / 14.0 155K395E (AAA→GAA) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base G (75/71);  total (80/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 159 total aligned reads displayed.

ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176323‑4176593
                                                                                                                                         |                                                                                                                                     
actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTgaa                                                                                                                                     >  1:517922/1‑140 (MQ=255)
actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTgaa                                                                                                                                     >  1:1867108/1‑140 (MQ=255)
 ctCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAg                                                                                                                                    >  2:1289916/1‑140 (MQ=255)
 ctCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAg                                                                                                                                    >  2:681941/1‑140 (MQ=255)
  tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGc                                                                                                                                   >  1:290038/1‑140 (MQ=255)
  tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGc                                                                                                                                   >  2:1512720/1‑140 (MQ=255)
  tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGc                                                                                                                                   >  2:1520555/1‑140 (MQ=255)
      gTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCg                                                                                                                               <  1:1354782/140‑1 (MQ=255)
       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  2:582134/1‑140 (MQ=255)
       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  2:1841820/1‑140 (MQ=255)
       tCGTTATGTAATCACCGTAGGTCCATACCTGCGTCTCCATCAGTGCTGTCTGCCGAAGAAAATTGCACTGGATCTCTTCAAACAGATCAACTACGGCAACCTGCAACACCGTCGTATTCCTACCAACATTGAAGATGCga                                                                                                                              >  2:233820/1‑140 (MQ=255)
         gTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaag                                                                                                                            >  1:1693452/1‑140 (MQ=255)
         gTTCTGTAATCACCGTAGGTCCATAACTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaag                                                                                                                            >  1:35365/1‑140 (MQ=255)
                  tCACAGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGtt                                                                                                                   <  2:1939607/140‑1 (MQ=255)
                    aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGa                                                                                                                 >  2:904803/1‑140 (MQ=255)
                    aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGa                                                                                                                 >  1:1560778/1‑140 (MQ=255)
                       gTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAgcg                                                                                                              >  1:1323673/1‑140 (MQ=255)
                         aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGcgcg                                                                                                            >  1:1505121/1‑140 (MQ=255)
                         aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGcgcg                                                                                                            >  2:1429863/1‑140 (MQ=255)
                            tCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCgaag                                                                                                         <  1:1841820/140‑1 (MQ=255)
                                tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  1:461759/1‑140 (MQ=255)
                                tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  1:1033808/1‑140 (MQ=255)
                                tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  2:1215256/1‑140 (MQ=255)
                                tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  2:1350066/1‑140 (MQ=255)
                                 aCCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                                                                    <  1:6312/140‑1 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTCCCTCCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               <  1:1808633/140‑1 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:442056/1‑140 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:1959220/1‑140 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               <  1:1392554/140‑1 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  2:797000/1‑140 (MQ=255)
                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  2:880342/1‑140 (MQ=255)
                                          tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:1978556/1‑140 (MQ=255)
                                          tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:385281/1‑140 (MQ=255)
                                           gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:681941/140‑1 (MQ=255)
                                           gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:240866/140‑1 (MQ=255)
                                                aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:1959220/140‑1 (MQ=255)
                                                aGTGAGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     >  2:328897/1‑140 (MQ=255)
                                                 gTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATcc                                                                                    >  2:1785748/1‑140 (MQ=255)
                                                 gTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATcc                                                                                    >  2:1135274/1‑140 (MQ=255)
                                                  tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGACGAAGCTGTCGTTTGGGATATCCt                                                                                   >  2:1392563/1‑140 (MQ=255)
                                                  tGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCt                                                                                   <  1:904803/140‑1 (MQ=255)
                                                       tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACg                                                                              >  1:1675600/1‑140 (MQ=255)
                                                       tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACg                                                                              >  1:377740/1‑140 (MQ=255)
                                                         tGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                            >  1:107259/1‑140 (MQ=255)
                                                          gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAg                                                                           <  1:860255/140‑1 (MQ=255)
                                                              aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGAGGAAGTTAt                                                                       >  2:1575452/1‑140 (MQ=255)
                                                              aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGATATCCTGGACGAAGTTAt                                                                       >  1:1238947/1‑140 (MQ=255)
                                                                    aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                 <  2:261236/140‑1 (MQ=255)
                                                                    aTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                 <  1:1372448/140‑1 (MQ=255)
                                                                     tGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                >  2:719606/1‑140 (MQ=255)
                                                                       gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAaca                                                              <  2:1697104/140‑1 (MQ=255)
                                                                        cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTCAAGCTGCGAAGAAAATGGTTGAGCGCGAAGCAGCTGTCGTTTGGGATATCCTGGACGTAGTTATCCGCGAacac                                                             >  1:411428/1‑140 (MQ=255)
                                                                           tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCg                                                          >  1:513365/1‑140 (MQ=255)
                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAATTTATCCGCGAACACCCgg                                                         <  1:1855465/140‑1 (MQ=255)
                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1497223/140‑1 (MQ=255)
                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1560778/140‑1 (MQ=255)
                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1520555/140‑1 (MQ=255)
                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1059879/140‑1 (MQ=255)
                                                                              aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  1:938237/140‑1 (MQ=255)
                                                                              aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  2:1094914/140‑1 (MQ=255)
                                                                                   ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                  >  2:86837/1‑140 (MQ=255)
                                                                                   ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                  >  2:86658/1‑140 (MQ=255)
                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              <  2:580621/140‑1 (MQ=255)
                                                                                        aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAcc                                             <  2:1323673/140‑1 (MQ=255)
                                                                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  1:1913284/1‑140 (MQ=255)
                                                                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  1:265082/1‑140 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:1978556/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:1867108/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:385281/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:467539/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:517922/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:1286104/140‑1 (MQ=255)
                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGACGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  2:148996/140‑1 (MQ=255)
                                                                                                 cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGa                                    <  2:42013/140‑1 (MQ=255)
                                                                                                      gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                               <  1:1429863/140‑1 (MQ=255)
                                                                                                        aaGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                             <  2:1693452/140‑1 (MQ=255)
                                                                                                           cTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCg                          <  2:1275071/140‑1 (MQ=255)
                                                                                                               aaCTGCGTGGTCTTGCTCCCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                      >  1:369127/1‑140 (MQ=255)
                                                                                                               aaCTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                      >  1:1620321/1‑140 (MQ=255)
                                                                                                                aCTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                     <  2:924687/140‑1 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCga                                                                                                           >  2:1550166/1‑50 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCga                                                                                                           <  1:1550166/50‑1 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCg                          >  1:1734846/1‑131 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  2:1930402/1‑140 (MQ=255)
                                                                                                                     gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  2:1084088/1‑61 (MQ=255)
                                                                                                                     gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               <  1:1084088/61‑1 (MQ=255)
                                                                                                                      tGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  1:999671/1‑98 (MQ=255)
                                                                                                                       ggTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGCCGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa              <  1:871349/140‑1 (MQ=255)
                                                                                                                       ggTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa              <  1:1135274/140‑1 (MQ=255)
                                                                                                                            tGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt         >  2:475306/1‑140 (MQ=255)
                                                                                                                            tGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt         >  1:601811/1‑140 (MQ=255)
                                                                                                                             gCTACCCCCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTGATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCGGGACCGTCTGGGTATCCAGGCAtt        <  1:1392563/140‑1 (MQ=255)
                                                                                                                             gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCact        <  1:887217/140‑3 (MQ=255)
                                                                                                                              cTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1822893/90‑1 (MQ=255)
                                                                                                                              cTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  1:1822893/1‑90 (MQ=255)
                                                                                                                                  caccaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGCTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc   <  2:1756484/140‑1 (MQ=255)
                                                                                                                                   accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  2:1713565/1‑85 (MQ=255)
                                                                                                                                   accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1713565/85‑1 (MQ=255)
                                                                                                                                   accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1225801/1‑140 (MQ=255)
                                                                                                                                   accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc  >  1:1635553/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176323‑4176593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A