Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F68 I0 R1	360	76.3	2813080	96.4%	2711809	135.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,176,460	A→G	54.9%	K395E (AAA→GAA)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,176,460	0	A	G	54.9%	36.8 / 163.7	111	K395E (AAA→GAA)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base A (28/22); new base G (25/36); total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 114 total aligned reads displayed.

ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176323‑4176593

actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTTGTCTTGCTACCACCATTaaa                                                                                                                                     >  1:328081/1‑140 (MQ=255)

actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTgaa                                                                                                                                     >  2:1036837/1‑140 (MQ=255)

actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTgaa                                                                                                                                     >  2:810165/1‑140 (MQ=255)

      gTCGTTCTGTACACACCGTAGGTCCAGACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCg                                                                                                                               <  1:1007229/140‑1 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCTAAGAAAATGGCACTGGAGCTGTTCAAACGGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCGGCga                                                                                                                              >  1:1302219/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  1:645762/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  1:1175440/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCga                                                                                                                              >  1:1342631/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCga                                                                                                                              >  2:864621/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCga                                                                                                                              >  2:1361521/1‑140 (MQ=255)

       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCga                                                                                                                              >  2:487088/1‑140 (MQ=255)

           tCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCgaagaa                                                                                                                          >  1:651428/1‑140 (MQ=255)

                    aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGACGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGGGAAGAAAATGGTTGa                                                                                                                 >  2:1259565/1‑140 (MQ=255)

                         aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGcgcg                                                                                                            >  2:1220809/1‑140 (MQ=255)

                         aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGcgcg                                                                                                            >  2:91449/1‑140 (MQ=255)

                             ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaaga                                                                                                        <  1:855988/140‑1 (MQ=255)

                                tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                     >  1:336051/1‑140 (MQ=255)

                                     gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGCAGAAAATGGTTGAGCGCGACGAAGCTGTCg                                                                                                >  1:190611/1‑140 (MQ=255)

                                     gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGACGAAAATGGTTGAGCGCGACGAAGCTGTCg                                                                                                >  2:807616/1‑140 (MQ=255)

                                     gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCg                                                                                                >  1:450882/1‑140 (MQ=255)

                                     gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCg                                                                                                >  1:1228631/1‑140 (MQ=255)

                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:1349944/1‑140 (MQ=255)

                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:445860/1‑140 (MQ=255)

                                      cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGt                                                                                               >  1:4152/1‑140 (MQ=255)

                                        tCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                             >  2:215503/1‑140 (MQ=255)

                                        tCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                             >  1:1155073/1‑140 (MQ=255)

                                        tCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                             >  1:78701/1‑140 (MQ=255)

                                        tCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                             >  2:550237/1‑140 (MQ=255)

                                          tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:490948/1‑140 (MQ=255)

                                          tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                           >  1:725142/1‑140 (MQ=255)

                                           ggaTCAGTGCGGTCTGCCGAAGAAAATTGCACTTGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAAGTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  2:815117/138‑1 (MQ=255)

                                           gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:1191362/140‑1 (MQ=255)

                                           gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:1247892/140‑1 (MQ=255)

                                           gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:492660/140‑1 (MQ=255)

                                            cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTTTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                         <  2:508437/140‑1 (MQ=255)

                                            cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                         <  2:114040/140‑1 (MQ=255)

                                                cgggCGGTCTGCCGAAGAAAAGGGCCCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:787182/137‑1 (MQ=255)

                                                aGTGCTGTCTGCCTAAGAAAATGGCACTGGATCTGTACAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGAATTGCTACCACCATTAAAGCTGGGAAGAAAATTGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:600551/140‑1 (MQ=255)

                                                aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:385684/140‑1 (MQ=255)

                                                aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:1404425/140‑1 (MQ=255)

                                                aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:257579/140‑1 (MQ=255)

                                                aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                     <  2:336051/140‑1 (MQ=255)

                                                aGTGCGGTCTGCCGAAGAAAAGGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGACGAAGCTGTCGTTTGGGATATc                                                                                     <  2:1403979/140‑1 (MQ=255)

                                                            cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGtt                                                                         >  2:957597/1‑140 (MQ=255)

                                                            cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGtt                                                                         >  2:1119098/1‑140 (MQ=255)

                                                                         aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                            <  1:22795/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAAGTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:659943/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAATCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1279924/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1349944/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:445860/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:675090/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:96425/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:91865/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:1220809/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGACGAAGCTGTCGTTTGGGAGATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:1068522/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  2:664129/140‑1 (MQ=255)

                                                                            ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:814219/140‑1 (MQ=255)

                                                                              aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  2:775022/140‑1 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTGGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              >  2:953714/1‑140 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              >  2:118056/1‑140 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAc                                              >  2:1124290/1‑140 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGCTACTGCTGAAc                                              >  2:1274270/1‑140 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  2:667622/96‑1 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          >  1:667622/1‑96 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  2:686561/1‑129 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:686561/129‑1 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         <  1:297767/129‑1 (MQ=255)

                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                         >  2:297767/1‑129 (MQ=255)

                                                                                         acGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGGTATCCTGGACGTCGTTATCCGCGATCACCCGGTACTGCTGAACCg                                            >  1:1040090/2‑140 (MQ=255)

                                                                                             tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                        >  2:908806/1‑140 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:1036837/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:1190878/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:1190840/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:469899/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:810165/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:908716/140‑1 (MQ=255)

                                                                                              cATCTACGGCAAGCAGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       <  1:230924/140‑1 (MQ=255)

                                                                                                    cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          <  1:685027/83‑1 (MQ=255)

                                                                                                    cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                          >  2:685027/1‑83 (MQ=255)

                                                                                                    cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                       >  2:73107/1‑134 (MQ=255)

                                                                                                      gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                               <  1:763518/140‑1 (MQ=255)

                                                                                                        aaGCTGGAACTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                             <  2:651428/140‑1 (MQ=255)

                                                                                                        aaGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                             <  2:1251808/140‑1 (MQ=255)

                                                                                                                aCTGCGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                     <  1:1344912/140‑1 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  1:1083484/102‑1 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       <  1:1080600/102‑1 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       >  2:1080600/1‑102 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                       >  2:1083484/1‑102 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  1:1360626/1‑140 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  1:604854/1‑140 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  2:763188/1‑140 (MQ=255)

                                                                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTAt                 >  2:761662/1‑140 (MQ=255)

                                                                                                                         tCTTGCTACCACCATTGAAGCTGCGAAGCAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg            <  1:1173895/140‑1 (MQ=255)

                                                                                                                         tCTTGCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg            <  1:139027/140‑1 (MQ=255)

                                                                                                                             gCTACCACCATTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt        <  1:1102991/140‑1 (MQ=255)

                                                                                                                                  caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc   <  1:82486/140‑1 (MQ=255)

                                                                                                                                   accaTTGAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTGTGGGATATCCTGGACGAAGTTATCCGCGAACCCCAGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCCTTTGAAcc  >  2:258147/1‑140 (MQ=255)

                                                                                                                                   accaTTAAAGCTGCGAAGACAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTCTCCAGGCATTTGAAcc  >  1:340346/1‑140 (MQ=255)

                                                                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg      <  1:139131/136‑1 (MQ=255)

                                                                                                                                   accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg      >  2:139131/1‑136 (MQ=255)

ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACC  >  CP009273/4176323‑4176593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A