Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F99 I1 R1	19	55.7	1970560	92.7%	1826709	142.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,817,170	C→A	E112* (GAA→TAA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,817,170	0	C	A	100.0%	149.9 / NA	48	E112* (GAA→TAA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/21); total (27/21)

CGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_pae_tpiA/2817027‑2817315

cGGTTGCACGCCCATGCGCTGCGCGCACGACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTCACGTGGACGGCAGCGACGTCGGTGTCAAAATACTGGCGGAGTTCGAGGTGCGCGGGCAATGCCTCAGCGATGGCGCTTTAACTcc                                                                                                                                              <  2:970861/149‑1 (MQ=255)

   ttGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCg                                                                                                                                           >  1:533086/1‑149 (MQ=255)

     gCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTCATCCCAGGTGCGCCAGCCATGCCTCAGCTATGGCGCTTTAACTCCCCGtt                                                                                                                                         >  2:115768/1‑149 (MQ=255)

       aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGGTTATGGTGATTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCGTTAACTCCCCGTTcc                                                                                                                                       >  1:646822/1‑149 (MQ=255)

         gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTCACGTGATCGGCAGCGACGACGGCGGCAAAATAATGGCGTAATACCAGGTCCGCCAGCCGTGCCTCCGCCATTGCCCTTTAACTCCCCGTTCCTa                                                                                                                                     >  2:983577/1‑149 (MQ=255)

             aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGc                                                                                                                                 <  2:924225/149‑1 (MQ=255)

                     cgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGc                                                                                                                         <  1:936165/149‑1 (MQ=255)

                          acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGACCGGCAGCGACGGCGGCGGCGAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCGTTAACTCGCCGTTCCTACAGCTATTGGGcgacgc                                                                                                                    >  1:117113/1‑148 (MQ=255)

                           caacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCgacgac                                                                                                                   >  1:332043/1‑149 (MQ=255)

                            aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGccc                                                                                                                  >  1:60451/1‑149 (MQ=255)

                              caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                <  1:174918/149‑1 (MQ=255)

                               aaaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATg                                                                                                               >  1:403152/1‑149 (MQ=255)

                                   aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGCAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCAATCTATCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGTCaa                                                                                                           <  1:880134/149‑1 (MQ=255)

                                    tgtgTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAAc                                                                                                          <  1:885361/149‑1 (MQ=255)

                                     gtgtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATCATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGCGCGACGACCACGCCACCt                                                                                                         >  2:360311/1‑149 (MQ=255)

                                        tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCCTGGGGCGACGGCCGTTCCCACtttt                                                                                                      >  1:758641/1‑149 (MQ=255)

                                          tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                >  1:395708/1‑137 (MQ=255)

                                          tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                <  2:395708/137‑1 (MQ=255)

                                          tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                    <  1:108733/149‑1 (MQ=255)

                                          tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                    <  1:944553/149‑1 (MQ=255)

                                           ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAAc                                                                                                                                                 <  1:434031/103‑1 (MQ=255)

                                                  gTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCGACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATca                                                                                            >  1:625721/1‑149 (MQ=255)

                                                   tttATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaa                                                                                           <  1:261774/149‑1 (MQ=255)

                                                       tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAag                                                                                       <  1:129877/149‑1 (MQ=255)

                                                        ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                      >  2:308945/1‑149 (MQ=255)

                                                         gtgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCAAGGTACGCCAGCAATGCCTCAGCGATGGCGCTTTACCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACTACATCAACAagag                                                                                     >  2:843498/1‑149 (MQ=255)

                                                                aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGAAAACCATCCCAACTTTTCACCCCATCAACAAGAGGAAGCgg                                                                              >  1:519192/1‑149 (MQ=255)

                                                                    gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                      <  1:774978/137‑1 (MQ=255)

                                                                    gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                      >  2:774978/1‑137 (MQ=255)

                                                                    gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg                                                                          >  1:936669/1‑149 (MQ=255)

                                                                       cggcggCGACGACGGCGTCCAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                       <  2:180847/149‑1 (MQ=255)

                                                                         gCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCt                                                                     >  1:243444/1‑149 (MQ=255)

                                                                               acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                               >  1:169859/1‑149 (MQ=255)

                                                                                 gccgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTCTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                             <  1:433878/147‑1 (MQ=255)

                                                                                 gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                             <  2:332043/149‑1 (MQ=255)

                                                                                      cGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCACGGCCTCAGCGATGGCGCTTTAACTCCCCGACCCTACAGCCATTGGGCGACGCCCCTGCCAACTTTTCACTACATCAACAAGAGGAGACGGTTCGACGCGATCccgccccaga                                                        >  2:596998/1‑143 (MQ=255)

                                                                                               aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACt                                               <  1:653520/149‑1 (MQ=255)

                                                                                                            cAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCACCAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttttt                                  >  1:933801/1‑149 (MQ=255)

                                                                                                                tGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCACCTTTTCACCACCTCAACAAGAGAAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAACGCTTCTGtttttcaacg                              >  2:423138/1‑144 (MQ=255)

                                                                                                                 gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                             <  1:308945/149‑1 (MQ=255)

                                                                                                                              cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg                >  2:104780/1‑149 (MQ=255)

                                                                                                                               tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAGGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               >  1:631568/1‑149 (MQ=255)

                                                                                                                               tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg               >  1:800063/1‑149 (MQ=255)

                                                                                                                               tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCCCTACCGCATGCgg               >  2:960694/1‑149 (MQ=255)

                                                                                                                                  gCGATCGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc            <  2:152163/149‑1 (MQ=255)

                                                                                                                                       tgcgcGTTAACTCCCCGTTCCCACAGCCATGAGGACACGACCCTGTCAACTTTTCGCCACCCTAATACGAAGAAGAAAAAAAAAGAAAACAAAAAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt       <  2:117113/148‑1 (MQ=255)

                                                                                                                                         gtcTTTAACTCCCGGTTCCGACAGCCATGGGGCGACGACCATGCCAACTTTTAACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGTATCCTTCTTACTGCTTCTGTTTTTTCACTCGCTAACGCATGCGGGTCGAGATCg     <  2:570146/147‑1 (MQ=255)

                                                                                                                                            tttAACTCCCCGTTCCTACAGCCATTGGACGACGACCATGCCAACTTTTCACAACAACAACAAGAGGAAGCGGCTCGACGCTATACAGGAGCATACTACTTACAGCTACTGTTTTTTGACCCGATAACGCATGCGGGTCGCGATCGGca  >  2:401411/1‑148 (MQ=255)

CGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC  >  NC_000913_3_pae_tpiA/2817027‑2817315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A