Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F106 I1 R1	19	81.9	2890106	93.9%	2713809	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,817,170	C→A	E112* (GAA→TAA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,817,170	0	C	A	98.7%	228.6 / NA	79	E112* (GAA→TAA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (39/38); minor base G (0/1); total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_pae_tpiA/2817026‑2817314

tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCCAAG‑CGTAAGCGATGGCGCTTTAACTc                                                                                                                                               >  1:889011/1‑149 (MQ=255)

   gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTcccc                                                                                                                                            >  1:58470/1‑149 (MQ=255)

       cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACACCCCGTTc                                                                                                                                        <  1:1438207/149‑1 (MQ=255)

       cACGCCCATACGCTCCGCGCACAACAAAAATGTGTCGGGCGCGGGTTTATGCTTTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                        <  1:192387/149‑1 (MQ=255)

        aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTAATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGttac                                                                                                                                       >  1:460322/1‑147 (MQ=255)

         cGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCt                                                                                                                                      <  1:63061/149‑1 (MQ=255)

          gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTa                                                                                                                                     <  2:811108/149‑1 (MQ=255)

            ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCGCGTTCCTGCa                                                                                                                                   >  1:944300/1‑149 (MQ=255)

              aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGc                                                                                                                                 >  2:986764/1‑149 (MQ=255)

                  gCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTACTACGCCAGCCGCTATCAGGG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACACCCact                                                                                                                             >  2:304135/1‑147 (MQ=255)

                   cTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTg                                                                                                                            >  1:214060/1‑149 (MQ=255)

                     gcgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg                                                                                                                          <  1:218153/149‑1 (MQ=255)

                     gcgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg                                                                                                                          <  2:45949/149‑1 (MQ=255)

                         gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGtt                                                                                                                                         <  2:517525/130‑1 (MQ=255)

                         gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGtt                                                                                                                                         >  1:517525/1‑130 (MQ=255)

                         gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                      <  2:1225647/149‑1 (MQ=255)

                         gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                      <  1:81952/149‑1 (MQ=255)

                           acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                                    >  2:1436360/1‑149 (MQ=255)

                           acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                                    >  1:846832/1‑149 (MQ=255)

                           acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                                    >  2:565517/1‑149 (MQ=255)

                             aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACCGCCATTGGGCGACGAcc                                                                                                                  >  1:89668/1‑149 (MQ=255)

                             aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc                                                                                                                  >  1:756188/1‑149 (MQ=255)

                               caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACCACCAt                                                                                                                >  2:1267339/1‑149 (MQ=255)

                               caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGAAGGCGTCAAACTAAGGGCGTAATAACAGAAAC‑CCCGCGACGTTCTGAACGAAAACGCTCTAACGCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                                >  2:1030040/1‑149 (MQ=255)

                                aaaaaGGTGTCTGGGGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATg                                                                                                               <  2:47282/149‑1 (MQ=255)

                                    aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCgacgac                                                                                                                   >  2:1130100/1‑141 (MQ=255)

                                    aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCgacgac                                                                                                                   <  1:1130100/141‑1 (MQ=255)

                                          cTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCGTACAGCCATTGGGCGACGACCATGCCAACTTTTc                                                                                                     <  2:1143966/149‑1 (MQ=255)

                                          cTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTc                                                                                                     <  1:1237473/149‑1 (MQ=255)

                                          cTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTc                                                                                                     <  2:1360305/149‑1 (MQ=255)

                                           tGGCGCTGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCACTTTGATTCCCCGTTAATATAATGTTTTGGCGACGACCATGCCAACTTTTca                                                                                                    <  1:1430481/149‑1 (MQ=255)

                                           tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                    <  2:104880/149‑1 (MQ=255)

                                            ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcac                                                                                                   <  1:1323243/149‑1 (MQ=255)

                                               gcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGCCCATGCCACCTTTTcaccac                                                                                                >  2:937388/1‑149 (MQ=255)

                                                cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGGGACGACCATGCCAACTTTTCACgaca                                                                                               >  1:324554/1‑149 (MQ=255)

                                                cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACcaca                                                                                               <  2:648287/149‑1 (MQ=255)

                                                             tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                  >  1:389112/1‑149 (MQ=255)

                                                                gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTCTCACCACATCAACAAGAGGAAGCg                                                                               <  1:1252460/149‑1 (MQ=255)

                                                                 aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                              <  1:973082/149‑1 (MQ=255)

                                                                   gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                            <  1:440794/149‑1 (MQ=255)

                                                                   gTGATCAGCAGCGACGACCGCGTCAAAATAAGAGCGTAATCCCAGGTGCCCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                            <  2:242377/149‑1 (MQ=255)

                                                                    tGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAGTCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTc                                                                           >  1:879533/1‑149 (MQ=255)

                                                                    tGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTc                                                                           <  2:876285/149‑1 (MQ=255)

                                                                    cgaactgCAGCGACGACGGCGTCAAAATAATGGCGTAATCACAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACAACCATGCCAACTTTTCACCACCTCAACAAGAGGAAGCGCTTc                                                                           >  2:540428/7‑149 (MQ=255)

                                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                         <  2:332357/149‑1 (MQ=255)

                                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATA‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCGCCAGATCAACAAAAAGAAGCGGTTcaa                                                                         >  1:123253/1‑147 (MQ=255)

                                                                       tCGGCAGCGACGGCGGCGACAAAAGAATGGCGTAACGCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                        <  1:145339/149‑1 (MQ=255)

                                                                       tCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                        <  1:326232/149‑1 (MQ=255)

                                                                        cGGCAGCGACGACGGCGTCAAAACAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                       <  2:1213191/149‑1 (MQ=255)

                                                                            aGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTAt                                                                   >  1:947319/1‑149 (MQ=255)

                                                                             gCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATc                                                                  <  2:846832/149‑1 (MQ=255)

                                                                               gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATccc                                                                >  2:816571/1‑148 (MQ=255)

                                                                                   acgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATcc                                                                 >  1:1028521/1‑144 (MQ=255)

                                                                                   acgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATcc                                                                 <  2:1028521/144‑1 (MQ=255)

                                                                                         tCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACCACCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCATCAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATAct                                                      <  2:406769/149‑1 (MQ=255)

                                                                                           aaaaTAGTGGATTAATACCACGTGCGCCAGCAATG‑CCTCAATAATTACGCTATAAATCCCCGTTCCTACAGCCATTGGGCGACGACCATGCTAACTTTTCAACTAATCATCAATAAAAAGCTTTTCGACGCTAGCCAGCAGTATActtc                                                    <  2:1150889/149‑1 (MQ=255)

                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACCGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCACCATActtc                                                    >  2:891149/1‑149 (MQ=255)

                                                                                                    gCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAAGCTATCAAGCAGCATAATTCTTACTGCtt                                           >  2:322954/1‑149 (MQ=255)

                                                                                                     cGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTc                                          <  2:214060/149‑1 (MQ=255)

                                                                                                            ccAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑ttttt                                  <  1:493463/149‑1 (MQ=255)

                                                                                                            ccAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGAACCTCCAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTCCTTACTGCTGCTG‑ttttt                                  >  2:239234/1‑149 (MQ=255)

                                                                                                                gTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTccc                              >  2:423768/1‑147 (MQ=255)

                                                                                                                    gcCAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACgcgc                          >  2:983171/1‑149 (MQ=255)

                                                                                                                    gcCAGCAATG‑CCTCAGCGATGGCGCTTAAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCGG‑TTTTTTCACgcgc                          >  2:1421665/1‑149 (MQ=255)

                                                                                                                     ccAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCt                         >  1:1049436/1‑149 (MQ=255)

                                                                                                                     ccAGCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCt                         >  1:1237661/1‑149 (MQ=255)

                                                                                                                        gCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAAc                      >  2:1338183/1‑149 (MQ=255)

                                                                                                                        gCAATG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAAc                      >  1:742955/1‑149 (MQ=255)

                                                                                                                            tG‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCAt                  >  2:259160/1‑149 (MQ=255)

                                                                                                                             g‑CCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATg                 <  2:1384498/149‑1 (MQ=255)

                                                                                                                               catCAGCGATAGCGCTTTTTTTCCCCGTTCCTACAGCCATCGGCTGACGACAATCACCACTACATACGCCAGTTTTATGATGTAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTTCACGCGCTAACGCATg                 >  2:1030158/3‑149 (MQ=255)

                                                                                                                                cTCCGCGATGGCGCTTTAACTCCCCGTTCCTTCAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCg               <  2:1343637/149‑1 (MQ=255)

                                                                                                                                cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTGTG‑TTTTTTCGCGCGCTGACGCGTGCg               >  1:383783/1‑149 (MQ=255)

                                                                                                                                 tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCgg              >  2:32377/1‑149 (MQ=255)

                                                                                                                                 tCAGCGATGGCCCCTGAACGCCCCCTTCCTACAGCCATGGGGCGACGACTATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCgg              <  1:659314/149‑1 (MQ=255)

                                                                                                                                   aGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGTCCATGCCAACTTTTCACCACTTCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGt            <  1:973990/149‑1 (MQ=255)

                                                                                                                                      gATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGTCga         <  1:1075180/149‑1 (MQ=255)

                                                                                                                                             cTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc  <  2:879533/149‑1 (MQ=255)

                                                                                                                                             cTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc  >  1:493721/1‑149 (MQ=255)

TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG‑CCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTG‑TTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_pae_tpiA/2817026‑2817314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A